AMICA/JAML Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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AMICA/JAML Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human AMICA/JAML
Leu20-Leu275
Accession # Q86YT9
Specificity
Detects human AMICA/JAML in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant mouse AMICA, recombinant human (rh) JAM-A, rhJAM-B, and rhJAM-C is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AMICA1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human AMICA/JAML Antibody (Catalog # AF3449)
ELISA Detection: Human AMICA/JAML Biotinylated Antibody (Catalog # BAF3449)
Standard:Recombinant Human AMICA/JAML Fc Chimera (Catalog # 3449-AM)
Publications
Read Publication using
AF3449 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMICA/JAML Antibody

  • adhesion molecule, interacts with CXADR antigen 1
  • AMICA
  • AMICA1
  • CREA7-1
  • Gm638
  • JAML

Background

AMICA (adhesion molecule, interacting with CXADR antigen 1), also known as JAML, is a 65 kDa, heavily glycosylated transmembrane protein that belongs to the junctional adhesion molecule (JAM) subset of the immunoglobulin superfamily (1). JAM family molecules contribute to intercellular connections within epithelial and endothelial cell layers, and mediate their interactions with various hemopoietic cells (1). The human AMICA cDNA encodes a 384 amino acid (aa) precursor that includes a 19 aa signal sequence, a 256 aa extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 98 aa cytoplasmic domain (2). Alternative splicing may generate isoforms with N- and C-terminal deletions. In contrast to other JAM family proteins, AMICA does not contain a cytoplasmic PDZ-binding motif (3). Within the ECD, human AMICA shares 58% and 63% aa sequence identity with mouse and rat AMICA, respectively. It shares 18%‑20% aa sequence identity with the ECDs of human JAM-A, -B, -C, and JAM4. AMICA is expressed on the surface of granulocytes and monocytes and is upregulated during the differentiation of myeloid leukemia cells (2, 3). A motif in the ECD, which promotes dimerization of other JAM family proteins, is required for surface localization of AMICA (2). AMICA mediates the adhesion of monocytes to endothelial cells (2) and neutrophil migration across epithelial cell monolayers (3). This latter function involves specific interactions of AMICA with the coxsackie virus and adenovirus receptor (CXADR) in epithelial tight junctions (3). In particular, the membrane proximal Ig-like domain of AMICA binds the membrane-distal Ig-like domain of CXADR (3). AMICA does not appear to interact homophilically, as neutrophils adhere to immobilized CXADR but not to immobilized AMICA (3).

  1. Mandell, K.J. and C.A. Parkos (2005) Adv. Drug Deliv. Rev. 57:857.
  2. Moog-Lutz, C. et al. (2003) Blood 102:3371.
  3. Zen, K. et al. (2005) Mol. Biol. Cell 16:2694.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AMICA/JAML Antibody (AF3449)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Secondary Antibodies

 

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Other Available Formats

Biotin BAF3449

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Bioinformatics

Gene Symbol AMICA1
Uniprot