Recombinant Human Integrin alpha V beta 3 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human Integrin alpha V beta 3 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Vitronectin (Catalog # 2308-VN) at 1 µg/mL can bind Recombinant Human Integrin alpha V beta 3 with an apparent KD <10 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha V beta 3 protein
Human Integrin alpha V subunit
(Phe31-Val992)
Accession # NP_002201
His-Pro GGGSGGGS  Acidic Tail
Human Integrin beta 3 subunit
(Gly27-Asp718)
Accession # AAA52589
His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus
Accession #
N-terminal Sequence
Phe31 & Gly27
Protein/Peptide Type
Recombinant Proteins
Gene
ITGAV
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
110.5 kDa ( alpha V subunit), 80.8 kDa ( beta 3 subunit).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
105-150 kDa, reducing conditions
Publications
Read Publications using
3050-AV in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha V beta 3 Protein, CF

  • antigen identified by monoclonal L230
  • CD51 antigen
  • CD51
  • Integrin alpha V beta 3
  • integrin alpha-V
  • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
  • MSK8
  • Vitronectin receptor subunit alpha
  • VNRADKFZp686A08142

Background

Integrin  alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (1‑3). The non‑covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N‑terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta‑propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine‑rich I‑EGF folds. The alpha V subunit domains termed thigh, calf‑1 and calf‑2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). The 962 aa human alpha V ECD(11) shares 92‑95% aa sequence identity with mouse, rat and bovine  alpha V while the 685 aa human beta 3 ECD(12) shares 95% aa identity with equine and canine, and 89‑92% aa identity with mouse, rat and porcine beta 3. Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M‑CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). alpha V beta 3 is involved in several other signaling pathways by direct interaction with receptor tyrosine kinases and ligands. For example, it cooperates with endothelial cell VEGF R2 in angiogenesis, and with IGF‑1 to promote cancer cell proliferation and invasiveness (13, 14). Also, cell entry of several viruses is mediated by alpha V beta 3 (4, 10).

  1. Hynes, R. O. (2002) Cell 110:673.
  2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
  3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
  4. Xiong, J. et al. (2001) Science 294:339.
  5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
  6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
  7. Djaffar, I. et al. (1994) Biochem. J. 300:69.
  8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
  9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
  11. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
  12. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
  13. Somanath, P.R. et al. (2009) Angiogenesis 12:177.
  14. Saegusa, J. et al. (2009) J. Biol. Chem. 284:24106.

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Publications for Integrin alpha V beta 3 (3050-AV)(8)

We have publications tested in 3 confirmed species: Human, N/A, Yeast.

We have publications tested in 2 applications: Binding Assay, Bioassay.


Filter By Application
Binding Assay
(2)
Bioassay
(5)
All Applications
Filter By Species
Human
(5)
N/A
(1)
Yeast
(1)
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Showing Publications 1 - 8 of 8.
Publications using 3050-AV Applications Species
Y Zur, L Rosenfeld, CA Keshelman, N Dalal, G Guterman-R, A Orenbuch, Y Einav, N Levaot, N Papo A dual-specific macrophage colony-stimulating factor antagonist of c-FMS and ?v?3 integrin for osteoporosis therapy PLoS Biol., 2018;16(8):e2002979. 2018 [PMID: 30142160] (Bioassay, Yeast) Bioassay Yeast
J Hedhli, A Czerwinski, M Schuelke, A P?oska, P Sowinski, L Hood, SB Mamer, JA Cole, P Czaplewska, M Banach, IT Dobrucki, L Kalinowski, P Imoukhuede, LW Dobrucki Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a Dimeric-cRGD Peptide for Targeted Imaging of ? V ? 3 Integrin Activity Sci Rep, 2017;7(1):3185. 2017 [PMID: 28600529] (Bioassay, Human) Bioassay Human
Brown A, Dysart M, Clarke K, Stabenfeldt S, Barker T Integrin alpha3beta1 Binding to Fibronectin Is Dependent on the Ninth Type III Repeat. J Biol Chem, 2015;290(42):25534-47. 2015 [PMID: 26318455] (Bioassay, Human) Bioassay Human
Hussain, Sajid, Rodriguez-Fernandez, Maria, Braun, Gary B, Doyle, Francis, Ruoslahti, Erkki Quantity and accessibility for specific targeting of receptors in tumours. Sci Rep, 2014;4(0):5232. 2014 [PMID: 24912981] (Bioassay, Human) Bioassay Human
Lung H, Man O, Yeung M, Ko J, Cheung A, Law E, Yu Z, Shuen W, Tung E, Chan S, Bangarusamy D, Cheng Y, Yang X, Kan R, Phoon Y, Chan K, Chua D, Kwong D, Lee A, Ji M, Lung M SAA1 polymorphisms are associated with variation in antiangiogenic and tumor-suppressive activities in nasopharyngeal carcinoma. Oncogene, 2015;34(7):878-89. 2015 [PMID: 24608426]
Kwon I, Hong S, Kim Y, Nam H, Kang S, Yang S, Heo J Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods: a FeCl3-induced thrombosis model in mice. PLoS ONE, 2013;8(11):e81165. 2013 [PMID: 24260554] (Binding Assay, N/A) Binding Assay N/A
Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer. J. Exp. Clin. Cancer Res., 2012;31(0):26. 2012 [PMID: 22444159] (Binding Assay, Human) Binding Assay Human
Jullienne B, Vigant F, Muth E, Chaligne R, Bouquet C, Giraudier S, Perricaudet M, Benihoud K Efficient delivery of angiostatin K1-5 into tumors following insertion of an NGR peptide into adenovirus capsid. Gene Ther., 2009;16(12):1405-15. 2009 [PMID: 19641530] (Bioassay, Human) Bioassay Human

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Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

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