Recombinant Human IL-3R alpha/CD123 Protein, CF

Recombinant Human IL-3 R alpha (Catalog # 301-R3/CF) binds Recombinant Human IL-3 in a functional ELISA. When Recombinant Human IL-3 R alpha is present at 1 µg/mL, the concentration of Recombinant Human IL‑3 (Catalog # 203-IL) that produces 50% of the optimal binding response is approximately 8‑40 ng/mL.

1 μg/lane of Recombinant Human IL-3 R alpha /CD123 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands 55 kDa and 58 kDa.

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human IL-3R alpha/CD123 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑3 R alpha /CD123 is present at 1 μg/mL, the concentration of Recombinant Human IL‑3 (Catalog # 203-IL) that produces 50% of the optimal binding response is found to be approximately 8‑40 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human IL-3 R alpha/CD123 protein
  Lys20-Arg305
• Accession #: P26951
• N-terminal Sequence: Lys20
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL3RA
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-3R alpha/CD123 Protein, CF

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL‑3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage‑committed cells. The biological effects of IL‑3 on the various cell types are mediated by the binding of IL‑3 to specific cell surface receptor complexes. The functional high‑affinity human IL‑3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL‑3 with low affinity. The beta  subunit does not bind IL‑3 by itself but is required for the high‑affinity binding of IL‑3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high‑affinity receptor complex for IL‑5 and GM‑CSF. Both the alpha and the beta  subunits are members of the cytokine receptor superfamily.

1. Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.

Publications for IL-3R alpha/CD123 (301-R3/CF)(2)

Publications using 301-R3/CF

• CD123-Engager T Cells as a Novel Immunotherapeutic for Acute Myeloid Leukemia Mol Ther, 2016-06-06;0(0):. 2016-06-06 [PMID: 27401038] (CAR-T (ELISA capture), Human)
• Li , G, Li , X, Wu , H, Yang , X, Zhang , Y, Chen , L, Wu , X, Cui , L, Wu , L, Luo , J, Liu , X Y CD123 targeting oncolytic adenoviruses suppress acute myeloid leukemia cell proliferation in vitro and in vivo. Blood Cancer J, 2014-03-21;4(0):e194. 2014-03-21 [PMID: 24658372] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IL3RA
• Uniprot:
  • P26951()