Recombinant Human IL-3R alpha/CD123 Fc Chimera Protein, CF

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-3 (203-IL) is present at 1.00 ug/mL, the concentration of Recombinant Human IL-3R alpha/CD123 Fc Chimera (Catalog # 11140-R3) that produces 50% of the binding response is 10.0-80.0 ng/mL.

2 μg/lane of Recombinant Human IL-3R alpha/CD123 Fc Chimera Protein (Catalog # 11140-R3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-91 kDa and 170-180 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-3R alpha/CD123 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑3 (Catalog # 203-IL) is present at 1.00 μg/mL, the concentration of Recombinant Human IL-3R alpha/CD123 Fc Chimera (Catalog # 11140-R3) that produces 50% of the binding response is 10.0-80.0 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-3R alpha protein
  

  Human IL-3R alpha (Thr19-Arg305) Accession # P26951.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P26951.1
• N-terminal Sequence: Thr19 and Lys20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 83-91 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-3R alpha/CD123 Fc Chimera Protein, CF

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

IL-3 receptor chain alpha (IL‑3R alpha) also known as CD123, is a member of the type 1 cytokine receptor family of proteins expressed mainly by activated T cells or mast cells (1-3). IL-3R alpha is a 60-70 kDa receptor component that binds IL-3 with low affinity and forms one-half of the IL-3 receptor complex. IL-3R alpha associates with the 130-140 kDa non-ligand binding IL-3R beta for high-affinity binding to IL-3 to form the heterodimeric receptor complex (4). IL-3R alpha consists of an extracellular domain (ECD) with an N-terminal Ig-like C2-type domain and a cytokine-binding domain containing two fibronectin type-III domains with a WSXWS motif, a helical transmembrane segment and a cytoplasmic domain (3). Within the mature ECD, human IL-3R alpha shares 30% and 31% amino acid sequence identity with mouse and rat IL-3R alpha, respectively.  An isoform lacking the N-terminal domain of ECD has been identified in mouse and human (5, 6). IL‑3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage‑committed cells (7). IL-3R alpha is expressed on multiple cell types, including endothelial cells, monocytes, eosinophils, basophils plus mast cells, and plasmacytoid CD4+ T cells (8). IL-3R alpha is over-expressed in acute myeloid leukemia and this is associated with a poor prognosis in AML (9). 

1. Broughton, S.E. et al. (2012) Immunol. Rev. 250:277.
2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
3. Broughton, S.E. et al. (2014) Acta Crystallogr F Struc Biol Commun. 70:358.
4. Broughton, S.E. et al. (2014) Cell Reports. 8:410.
5. Chen, J. et al. (2009) J. Biol. Chem. 284:5763.
6. Hamming, O.J. et al. (2012) J Biol Chem. 287:9454.
7. Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.
8. Michalska, A. et al. (2020) Postepy Dermatol Alergol. 37:299.
9. Testa, U. et al. (2002) Blood 100:2980.

Bioinformatics

• Uniprot:
  • P26951.1()