Recombinant Human IL-33 Protein


1 μg/lane of Recombinant Human IL-33 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Recombinant Human IL-33 (Catalog #3625‑IL) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.06-0.24 ng/mL.

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Recombinant Human IL-33 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.06-0.24 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
E. coli-derived human IL-33 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
3625-IL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-33 Protein

  • C9orf26
  • C9orf26chromosome 9 open reading frame 26 (NF-HEV)
  • DKFZp586H0523
  • DVS27
  • DVS27-related protein
  • IL1F11
  • IL-1F11
  • IL33
  • IL-33
  • interleukin 33
  • Interleukin-1 family member 11
  • interleukin-33
  • NF-HEV
  • Nuclear factor from high endothelial venules
  • RP11-575C20.2


IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF‑HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL‑33 was identified based on sequence and structural homology with IL‑1 family cytokines (3). DVS 27, NF‑HEV, and IL‑33 share 100% amino acid sequence identity. IL‑33 is constitutively expressed in smooth muscle and airway epithelia. It is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL‑1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL‑1, IL‑33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full length IL‑33 contains a predicted bipartite nuclear localization sequence and a homeodomain‑like helix‑turn‑helix DNA binding domain. By immunofluorescence, full length IL‑33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL‑33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL‑33 and ST2L with IL‑1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL‑33 in vitro induces IL‑5 and IL‑13 secretion (3). In vivo administration of mature IL‑33 promotes increased production of IL‑5, IL‑13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL‑33 share
52‑58% aa sequence identity with mouse and rat IL‑33. Human IL‑33 shares less than 20% aa sequence identity with other IL‑1 family proteins.

  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

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Publications for IL-33 (3625-IL)(32)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Flow, In Vivo.

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Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using 3625-IL Applications Species
K Matsumoto, H Kouzaki, H Kikuoka, T Kato, I Tojima, S Shimizu, T Shimizu Soluble ST2 suppresses IL-5 production by human basophilic KU812 cells, induced by epithelial cell-derived IL-33 Allergol Int, 2018;0(0):. 2018 [PMID: 29941231] (Bioassay, Human) Bioassay Human
J Liu, W Wang, L Wang, S Chen, B Tian, K Huang, CJ Corrigan, S Ying, W Wang, C Wang IL-33 Initiates Vascular Remodelling in Hypoxic Pulmonary Hypertension by up-Regulating HIF-1? and VEGF Expression in Vascular Endothelial Cells EBioMedicine, 2018;0(0):. 2018 [PMID: 29921553] (Bioassay, Human) Bioassay Human
Q Miao, Y Wang, YG Liu, YX Ren, H Guan, Z Li, W Xu, L Xiang Seasonal variation in circulating group 2 innate lymphoid cells in mugwort-allergic asthmatics during and outside pollen season Allergy Asthma Clin Immunol, 2018;14(0):6. 2018 [PMID: 29449864] (Bioassay, Human) Bioassay Human
JE Miller, SP Monsanto, SH Ahn, K Khalaj, AT Fazleabas, SL Young, BA Lessey, M Koti, C Tayade Interleukin-33 modulates inflammation in endometriosis Sci Rep, 2017;7(1):17903. 2017 [PMID: 29263351] (Bioassay, Human) Bioassay Human
K Wang, S Shan, Z Yang, X Gu, Y Wang, C Wang, T Ren IL-33 blockade suppresses tumor growth of human lung cancer through direct and indirect pathways in a preclinical model Oncotarget, 2017;8(40):68571-68582. 2017 [PMID: 28978138] (Bioassay, Human) Bioassay Human
D Díaz-Jimén, L Núñez, M De la Fuen, K Dubois-Cam, H Sepúlveda, M Montecino, A Torres-Riq, P García-Gon, J Chnaiderma, A Vossenkamp, TT MacDonald, D Simian, MJ González, JA Cidlowski, R Quera, MA Hermoso A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis Sci Rep, 2017;7(1):10180. 2017 [PMID: 28860510] (Bioassay, Human) Bioassay Human
JF Zhang, P Wang, YJ Yan, Y Li, MW Guan, JJ Yu, XD Wang IL?33 enhances glioma cell migration and invasion by upregulation of MMP2 and MMP9 via the ST2-NF-?B pathway Oncol. Rep., 2017;38(4):2033-2042. 2017 [PMID: 28849217] (Bioassay, Human) Bioassay Human
XH Wang, W Liu, DX Fan, WT Hu, MQ Li, XY Zhu, LP Jin IL?33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin ?4?1 and CD62L Mol Med Rep, 2017;0(0):. 2017 [PMID: 28765940] (Bioassay, Human) Bioassay Human
V Lombardi, S Luce, H Moussu, L Morizur, C Gueguen, C Neukirch, S Chollet-Ma, L Mascarell, M Aubier, V Baron-Bodo, P Moingeon Effector and regulatory dendritic cells display distinct patterns of miRNA expression Immun Inflamm Dis, 2017;0(0):. 2017 [PMID: 28497578] (Bioassay, Human) Bioassay Human
A Taracanova, M Alevizos, A Karagkouni, Z Weng, E Norwitz, P Conti, SE Leeman, TC Theoharide SP and IL-33 together markedly enhance TNF synthesis and secretion from human mast cells mediated by the interaction of their receptors Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28461492] (Human) Human
Show All 32 Publications.

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Blogs on IL-33.

Interleukin 33 (IL-33) - A dual function cytokine
IL-33 is a member of the interleukin family of cytokines that regulates a wide variety of cellular functions. Its receptor is ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and the IL-1R accessory...  Read full blog post.

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Gene Symbol IL33