Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF

Measured by its ability to inhibit IL-18-induced response of KG-1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (816-LR). The ED50 for this effect is 0.300-2.40 µg/mL in the presence of 30.0 µg/mL of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera and 40 ng/mL of Recombinant Human IL-18/IL-1F4 Protein.

2 μg/lane of Recombinant Human IL‑18 R beta /IL‑1 R7 His-tag Protein (Catalog # 11210-AP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 46-61 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit IL-18-induced response of KG-1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (Catalog # 816-LR) . The ED50 for this effect is 0.300-2.40 µg/mL in the presence of 30.0 µg/mL of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera and 40 ng/mL of Recombinant Human IL-18/IL-1F4 Protein.
• Source: Human embryonic kidney cell, HEK293-derived human IL-18 R beta/IL-1 R7 protein
  Phe20-Arg356, with a C-terminal 6-His tag
• Accession #: O95256.1
• N-terminal Sequence: Phe20
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 46-61 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-18 R beta/IL-1 R7 His-tag Protein, CF

• Accessory protein-like
• AcPL
• CD218 antigen-like family member B
• CD218b antigen
• CD218b
• CDw218b
• IL-1 R7
• IL-18 R beta
• IL-18 receptor accessory protein
• IL-18 receptor beta
• IL18R beta
• IL-18RAcP
• IL18RAP
• IL18RB
• IL-18Rb
• IL-18Rbeta
• IL-18R-beta
• IL-1R accessory protein-like
• IL1R7
• IL-1R7
• IL-1R-7
• IL-1RAcPL
• interleukin 18 receptor accessory protein
• Interleukin-1 receptor 7
• interleukin-18 receptor accessory protein
• Interleukin-18 receptor accessory protein-like
• Interleukin-18 receptor beta
• MGC120589
• MGC120590

Background

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
2. Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Bioinformatics

• Uniprot:
  • O95256.1()