Recombinant Human IL-18 R beta/IL-1 R7 His Avi Protein, CF

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera Protein (816-LR) is coated at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human IL‑18R beta /IL‑1R7 His-tag Avi-tag Protein (Catalog # AVI11210) binds with an ED50 of 2.00-40.0 ng/mL.

2 μg/lane of Recombinant Human IL-18 R beta/IL-1 R7 His-tag Avi-tag Protein (Catalog # AVI11210) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-61 kDa.

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-18 R beta/IL-1 R7 His Avi Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (Catalog # 816-LR) is coated at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human IL‑18R beta /IL‑1R7 His-tag Avi-tag (Catalog # AVI11210) binds with an ED50 of 2.00-40.0 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human IL-18 R beta/IL-1 R7 protein
  

  Human IL-18 R beta/IL-1 R7 (Phe20-Arg356) Accession # O95256.1	6-His tag	Avi-tag
  N-terminus		C-terminus

• Accession #: O95256.1
• N-terminal Sequence: Phe20
• Structure / Form: Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 48-61 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-18 R beta/IL-1 R7 His Avi Protein, CF

• Accessory protein-like
• AcPL
• CD218 antigen-like family member B
• CD218b antigen
• CD218b
• CDw218b
• IL-1 R7
• IL-18 R beta
• IL-18 receptor accessory protein
• IL-18 receptor beta
• IL18R beta
• IL-18RAcP
• IL18RAP
• IL18RB
• IL-18Rb
• IL-18Rbeta
• IL-18R-beta
• IL-1R accessory protein-like
• IL1R7
• IL-1R7
• IL-1R-7
• IL-1RAcPL
• interleukin 18 receptor accessory protein
• Interleukin-1 receptor 7
• interleukin-18 receptor accessory protein
• Interleukin-18 receptor accessory protein-like
• Interleukin-18 receptor beta
• MGC120589
• MGC120590

Background

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon. Our Avi-tag Biotinylated IL-18 R beta /IL-1R7His-tag features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
2. Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Bioinformatics

• Uniprot:
  • O95256.1()