Recombinant Human IL-17A Protein, CF

Recombinant Human IL-17 (Catalog # 317-ILB) induces CXCL1/GRO alpha secretion in HT‑29human colon adenocarcinoma cells. The ED50 for this effect is 0.4‑4 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-17A Protein, CF Summary

• Details of Functionality: Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.4-4 ng/mL. Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED₅₀ for this effect is 1.5-7.5 ng/mL.
• Source: E. coli-derived human IL-17/IL-17A protein
  Ile20-Ala155, with an N-terminal Met
• Accession #: Q16552
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL17A
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 16 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HCl.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-17A Protein, CF

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17A shares 60% amino acid sequence identity with mouse and rat IL-17A (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15).
IL-17A has been shown to exert either tumorigenic or anti-tumor effects (16, 17).

1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
3. Yao, Z. et al. (1995) J. Immunol. 155:5483.
4. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
8. Rong, Z. et al. (2009) Cell Res. 19:208.
9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
13. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
14. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
15. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
16. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
17. Kryczek, I. et al. (2009) Blood 114:357.

Publications for IL-17/IL-17A (317-ILB)(95)

Publications using 317-ILB

• Jhanji, M;Krall, CL;Guevara, A;Yoon, B;Sajish, M;Boccuto, L;Lizarraga, SB; The intersection of inflammation and DNA damage as a novel axis underlying the pathogenesis of autism spectrum disorders bioRxiv : the preprint server for biology 2024-12-11 [PMID: 39713319] (Bioassay, Human)
• Zhang, C;Zhang, Q;Chen, J;Li, H;Cheng, F;Wang, Y;Gao, Y;Zhou, Y;Shi, L;Yang, Y;Liu, J;Xue, K;Zhang, Y;Yu, H;Wang, D;Hu, L;Wang, H;Sun, X; Neutrophils in nasal polyps exhibit transcriptional adaptation and proinflammatory roles that depend on local polyp milieu JCI insight 2024-11-22 [PMID: 39361432] (Bioassay, Human)
• Caratis, F;Opie?ka, M;Hausmann, M;Velasco-Estevez, M;Rojek, B;de Vallière, C;Seuwen, K;Rogler, G;Karaszewski, B;Rutkowska, A; The proton-sensing receptors TDAG8 and GPR4 are differentially expressed in human and mouse oligodendrocytes: Exploring their role in neuroinflammation and multiple sclerosis PloS one 2024-03-25 [PMID: 38527054] (Bioassay, Human)
• Ma, F;Plazyo, O;Billi, AC;Tsoi, LC;Xing, X;Wasikowski, R;Gharaee-Kermani, M;Hile, G;Jiang, Y;Harms, PW;Xing, E;Kirma, J;Xi, J;Hsu, JE;Sarkar, MK;Chung, Y;Di Domizio, J;Gilliet, M;Ward, NL;Maverakis, E;Klechevsky, E;Voorhees, JJ;Elder, JT;Lee, JH;Kahlenberg, JM;Pellegrini, M;Modlin, RL;Gudjonsson, JE; Single cell and spatial sequencing define processes by which keratinocytes and fibroblasts amplify inflammatory responses in psoriasis Nature communications 2023-06-12 [PMID: 37308489] (Bioassay, Human)
• Arnold, KA;Peterson, LF;Beck, LA;Brewer, MG; JAK Signaling Is Critically Important in Cytokine-Induced Viral Susceptibility of Keratinocytes International journal of molecular sciences 2023-05-25 [PMID: 37298195] (Bioassay, Human)
• G Xiao, R Kumar, Y Komuro, J Burguet, V Kakarla, I Azizkhania, SA Sheth, CK Williams, XR Zhang, M Macknicki, A Brumm, R Kawaguchi, P Mai, N Kaneko, HV Vinters, ST Carmichael, LA Havton, C DeCarli, JD Hinman IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury Cell Reports, 2022-12-20;41(12):111848. 2022-12-20 [PMID: 36543124] (Bioassay, Human)
• Shibnath Ghatak, Vincent C. Hascall, Nikos Karamanos, Roger R. Markwald, Suniti Misra Interplay Between Chemotherapy-Activated Cancer Associated Fibroblasts and Cancer Initiating Cells Expressing CD44v6 Promotes Colon Cancer Resistance Frontiers in Oncology 2022-08-02 [PMID: 35982950] (Bioassay, Human)
• S Shao, J Chen, WR Swindell, LC Tsoi, X Xing, F Ma, R Uppala, MK Sarkar, O Plazyo, AC Billi, R Wasikowski, KM Smith, P Honore, VE Scott, E Maverakis, JM Kahlenberg, G Wang, NL Ward, PW Harms, JE Gudjonsson Phospholipase A2 enzymes represent a shared pathogenic pathway in psoriasis and pityriasis rubra pilaris JCI Insight, 2021-10-22;0(0):. 2021-10-22 [PMID: 34491907] (Bioassay, Human)
• P Ebner-Peki, L Krisch, M Wolf, S Hochmann, A Hoog, B Vári, K Muigg, R Poupardin, C Scharler, S Schmidhube, E Russe, H Stachelsch, A Schneeberg, K Schallmose, D Strunk Self-assembly of differentiated progenitor cells facilitates spheroid human skin organoid formation and planar skin regeneration Theranostics, 2021-07-25;11(17):8430-8447. 2021-07-25 [PMID: 34373751] (Bioassay, Human)
• K Jamal Jame, WJ Gallert, SD Yanik, S Panek, J Kronsbein, D Jungck, A Koch, J Knobloch Biomarkers for Comorbidities Modulate the Activity of T-Cells in COPD International Journal of Molecular Sciences, 2021-07-02;22(13):. 2021-07-02 [PMID: 34281240] (Bioassay, Human)
• W Soonthornc, P Tangtanata, K Meesilpavi, V Dalm, P Kueanjinda, J Wongpiyabo MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene Scientific Reports, 2021-07-09;11(1):14186. 2021-07-09 [PMID: 34244572] (Bioassay, Human)
• L Gorchs, S Ahmed, C Mayer, A Knauf, C Fernández, M Svensson, R Heuchel, E Rangelova, P Bergman, H Kaipe The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity Sci Rep, 2020-10-15;10(1):17444. 2020-10-15 [PMID: 33060625] (Human)
• J Yan, SP Pandey, BJ Barnes, JR Turner, C Abraham T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation Cell Rep, 2020-06-30;31(13):107820. 2020-06-30 [PMID: 32610123] (Bioassay, Human)
• RA Lu, AA Zeki, S Ram-Mohan, N Nguyen, Y Bai, K Chmiel, S Pecic, X Ai, R Krishnan, CC Ghosh Inhibiting Airway Smooth Muscle Contraction Using Pitavastatin: A Role for the Mevalonate Pathway in Regulating Cytoskeletal Proteins Front Pharmacol, 2020-05-06;11(0):469. 2020-05-06 [PMID: 32435188] (Cell Culture, Human)
• J Lu, G Jiang, Y Wu, S Antony, JL Meitzler, A Juhasz, H Liu, K Roy, H Makhlouf, R Chuaqui, D Butcher, MM Konaté, JH Doroshow NADPH oxidase 1 is highly expressed in human large and small bowel cancers PLoS ONE, 2020-05-19;15(5):e0233208. 2020-05-19 [PMID: 32428030] (Bioassay, Human)
• L Mercurio, D Lulli, F Mascia, E Dellambra, C Scarponi, M Morelli, C Valente, ML Carbone, S Pallotta, G Girolomoni, C Albanesi, S Pastore, S Madonna Intracellular Insulin-like growth factor binding protein 2 (IGFBP2) contributes to the senescence of keratinocytes in psoriasis by stabilizing cytoplasmic p21 Aging (Albany NY), 2020-04-17;12(8):6823-6851. 2020-04-17 [PMID: 32302288] (Cell Culture, Human)
• D Deli?, K Wolk, R Schmid, O Gabrielyan, D Christou, K Rieber, M Rolser, I Jakob, F Wiech, M Griesser, C Wohnhaas, G Kokolakis, E Witte-Händ, P Baum, R Sabat Integrated microRNA/mRNA expression profiling of the skin of psoriasis patients J. Dermatol. Sci., 2019-11-30;0(0):. 2019-11-30 [PMID: 31843230] (Bioassay, Human)
• X Xu, H Fukui, Y Ran, X Wang, Y Inoue, N Ebisudani, H Nishimura, T Tomita, T Oshima, J Watari, H Kiyama, H Miwa The Link between Type III Reg and STAT3-Associated Cytokines in Inflamed Colonic Tissues Mediators Inflamm., 2019-11-06;2019(0):7859460. 2019-11-06 [PMID: 31780871] (Bioassay, Human)
• C Merriman, D Fu Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress J. Biol. Chem., 2019-10-07;0(0):. 2019-10-07 [PMID: 31591269] (Bioassay, Human)
• Y Wu, MM Konaté, J Lu, H Makhlouf, R Chuaqui, S Antony, JL Meitzler, MJ Difilippan, H Liu, A Juhasz, G Jiang, I Dahan, K Roy, JH Doroshow IL-4 and IL-17A Cooperatively Promote Hydrogen Peroxide Production, Oxidative DNA Damage, and Upregulation of Dual Oxidase 2 in Human Colon and Pancreatic Cancer Cells J. Immunol., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548328] (Bioassay, Human)
• JC Chamcheu, S Esnault, VM Adhami, AL Noll, S Banang-Mbe, T Roy, SS Singh, S Huang, KG Kousoulas, H Mukhtar Fisetin, a 3,7,3&#039;,4&#039;-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models Cells, 2019-09-15;8(9):. 2019-09-15 [PMID: 31540162] (ELISA Standard, Human)
• X Qin, M Liu, S Zhang, C Wang, T Zhang The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis Cytokine, 2019-03-01;117(0):84-90. 2019-03-01 [PMID: 30831444] (Bioassay, Human)
• L Mercurio, M Morelli, C Scarponi, EZ Eisenmesse, N Doti, G Pagnanelli, E Gubinelli, C Mazzanti, A Cavani, M Ruvo, CA Dinarello, C Albanesi, S Madonna IL-38 has an anti-inflammatory action in psoriasis and its expression correlates with disease severity and therapeutic response to anti-IL-17A treatment Cell Death Dis, 2018-10-30;9(11):1104. 2018-10-30 [PMID: 30377293] (Bioassay, Human)
• M Croes, MC Kruyt, WM Groen, KMA van Dorenm, WJA Dhert, FC Öner, J Alblas Interleukin 17 enhances bone morphogenetic protein-2-induced ectopic bone formation Sci Rep, 2018-05-08;8(1):7269. 2018-05-08 [PMID: 29740080] (In Vivo, Rabbit)
• A Rutkowska, DR Shimshek, AW Sailer, KK Dev EBI2 regulates pro-inflammatory signalling and cytokine release in astrocytes Neuropharmacology, 2018-01-31;133(0):121-128. 2018-01-31 [PMID: 29374507] (Bioassay, Human, Mouse, Rat)
• M Carretta, AZ Brouwers-V, M Bosman, SJ Horton, JHA Martens, E Vellenga, JJ Schuringa BRD3/4 inhibition and FLT3-ligand deprivation target pathways that are essential for the survival of human MLL-AF9+ leukemic cells PLoS ONE, 2017-12-14;12(12):e0189102. 2017-12-14 [PMID: 29240787] (Bioassay, Human)
• Y Wang, JB Mumm, R Herbst, R Kolbeck, Y Wang IL-22 Increases Permeability of Intestinal Epithelial Tight Junctions by Enhancing Claudin-2 Expression J. Immunol., 2017-09-22;0(0):. 2017-09-22 [PMID: 28939759] (Bioassay, Human)
• SL Fossum, MJ Mutolo, A Tugores, S Ghosh, SH Randell, LC Jones, SH Leir, A Harris Ets Homologous Factor (EHF) has Critical Roles in Epithelial Dysfunction in Airway Disease J. Biol. Chem., 2017-05-01;0(0):. 2017-05-01 [PMID: 28461336] (Bioassay, Human)
• KA Cho, M Park, YH Kim, SY Woo Th17 cell-mediated immune responses promote mast cell proliferation by triggering stem cell factor in keratinocytes Biochem. Biophys. Res. Commun., 2017-04-27;0(0):. 2017-04-27 [PMID: 28456630] (Bioassay, Human)
• JS Ainscough, T Macleod, D McGonagle, R Brakefield, JM Baron, A Alase, M Wittmann, M Stacey Cathepsin S is the major activator of the psoriasis-associated proinflammatory cytokine IL-36? Proc. Natl. Acad. Sci. U.S.A, 2017-03-13;114(13):E2748-E2757. 2017-03-13 [PMID: 28289191] (Bioassay, Human)
• BF Wang, PP Cao, ZC Wang, ZY Li, ZZ Wang, J Ma, B Liao, YK Deng, XB Long, K Xu, H Wang, H Wang, M Zeng, X Lu, Z Liu Interferon-? induced insufficient autophagy contributes to p62-dependent apoptosis of epithelial cells in chronic rhinosinusitis with nasal polyps Allergy, 2017-04-04;0(0):. 2017-04-04 [PMID: 28258963] (Bioassay, Human)
• Dobrina N Hull Increase in circulating Th17 cells during anti-TNF therapy is associated with ultrasonographic improvement of synovitis in rheumatoid arthritis Arthritis Res. Ther, 2016-12-23;18(1):303. 2016-12-23 [PMID: 28010726] (ELISA (Standard), Human)
• Qingshui Huang Interleukin-17A-Induced Epithelial-Mesenchymal Transition of Human Intrahepatic Biliary Epithelial Cells: Implications for Primary Biliary Cirrhosis Tohoku J. Exp. Med, 2016-12-01;240(4):269-275. 2016-12-01 [PMID: 27916760] (Bioassay, Human)
• Robert Sabat MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations Mediators Inflamm., 2016-10-23;2016(0):4097574. 2016-10-23 [PMID: 27843200] (Bioassay, Human)
• Hiroaki Yamazaki A Novel PEGylation Method for Improving the Pharmacokinetic Properties of Anti-Interleukin-17A RNA Aptamers Nucleic Acid Ther, 2016-11-09;0(0):. 2016-11-09 [PMID: 27827561] (Bioassay, Mouse)
• PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells Sci Rep, 2016-11-09;6(0):36795. 2016-11-09 [PMID: 27827428] (Bioassay, Human)
• Inhibiting complex IL-17A and IL-17RA interactions with a linear peptide Sci Rep, 2016-05-17;6(0):26071. 2016-05-17 [PMID: 27184415] (ELISA Development (Detection), Complex Species Category)
• TM Garcia-Bat, E Kim, F Concha-Ben, S Trivedi, RB Mailliard, A Gambotto, RL Ferris Enhanced Cytotoxic CD8 T Cell Priming Using Dendritic Cell-Expressing Human Papillomavirus-16 E6/E7-p16INK4 Fusion Protein with Sequenced Anti-Programmed Death-1 J. Immunol, 2016-02-05;196(6):2870-8. 2016-02-05 [PMID: 26851223] (Bioassay, Human)
• Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis. J Exp Med, 2015-04-27;212(5):619-31. 2015-04-27 [PMID: 25918342] (Bioassay, Human)
• Kwofie K, Scott M, Rodrigues R, Guerette J, Radford K, Nair P, Richards C Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M. Respir Res, 2015-02-07;16(0):14. 2015-02-07 [PMID: 25849622] (Bioassay, Human)
• Banas M, Zegar A, Kwitniewski M, Zabieglo K, Marczynska J, Kapinska-Mrowiecka M, LaJevic M, Zabel B, Cichy J The expression and regulation of chemerin in the epidermis. PLoS ONE, 2015-02-06;10(2):e0117830. 2015-02-06 [PMID: 25659101] (Bioassay, Human)
• Adams , Kelly M, Abraham , Valsamma, Spielman , Daniel, Kolls , Jay K, Rubenstein , Ronald C, Conner , Gregory, Cohen , Noam A, Kreindler , James L IL-17A induces Pendrin expression and chloride-bicarbonate exchange in human bronchial epithelial cells. PLoS ONE, 2014-08-20;9(8):e103263. 2014-08-20 [PMID: 25141009] (Bioassay, Human)
• Wu L, Wang C, Boisson B, Misra S, Rayman P, Finke J, Puel A, Casanova J, Li X The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling. J Immunol, 2014-07-14;193(4):1590-9. 2014-07-14 [PMID: 25024377] (Bioassay, Human)
• Rabeony H, Petit-Paris I, Garnier J, Barrault C, Pedretti N, Guilloteau K, Jegou J, Guillet G, Huguier V, Lecron J, Bernard F, Morel F Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M. PLoS ONE, 2014-07-10;9(7):e101937. 2014-07-10 [PMID: 25010647] (Bioassay, Human)
• Wang Y, Wu H, Wu X, Bian Z, Gao Q Interleukin 17A promotes gastric cancer invasiveness via NF-kappaB mediated matrix metalloproteinases 2 and 9 expression. PLoS ONE, 2014-06-06;9(6):e96678. 2014-06-06 [PMID: 24905806] (Bioassay, Human)
• Xia , Wentong, Bai , Jing, Wu , Xingmei, Wei , Yi, Feng , Shaoyan, Li , Lei, Zhang , Jia, Xiong , Guanxia, Fan , Yunping, Shi , Jianbo, Li , Huabin Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway. PLoS ONE, 2014-06-03;9(6):e98915. 2014-06-03 [PMID: 24892823] (Bioassay, Human)
• MacLeod A, Rudolph R, Corriden R, Ye I, Garijo O, Havran W Skin-resident T cells sense ultraviolet radiation-induced injury and contribute to DNA repair. J Immunol, 2014-05-07;192(12):5695-702. 2014-05-07 [PMID: 24808367] (Bioassay, Human)
• Ardeljan , Daniel, Wang , Yujuan, Park , Stanley, Shen , Defen, Chu , Xi Kathy, Yu , Cheng-Ro, Abu-Asab , Mones, Tuo , Jingshen, Eberhart , Charles, Olsen , Timothy, Mullins , Robert F, White , Gary, Wadsworth , Sam, Scaria , Abraham, Chan , Chi-Chao Interleukin-17 retinotoxicity is prevented by gene transfer of a soluble interleukin-17 receptor acting as a cytokine blocker: implications for age-related macular degeneration. PLoS ONE, 2014-04-29;9(4):e95900. 2014-04-29 [PMID: 24780906] (Bioassay, Human, Primate - Chlorocebus pygerythrus (Vervet Monkey))
• Chiricozzi , Andrea, Nograles , Kristine, Johnson-Huang , Leanne M, Fuentes-Duculan , Judilyn, Cardinale , Irma, Bonifacio , Kathleen, Gulati , Nicholas, Mitsui , Hiroshi, Guttman-Yassky , Emma, Suarez-Farinas , Mayte, Krueger , James G IL-17 induces an expanded range of downstream genes in reconstituted human epidermis model. PLoS ONE, 2014-02-28;9(2):e90284. 2014-02-28 [PMID: 24587313] (Bioassay, Human)
• Chen Z, Kim S, Chamberlain N, Pickens S, Volin M, Volkov S, Arami S, Christman J, Prabhakar B, Swedler W, Mehta A, Sweiss N, Shahrara S The novel role of IL-7 ligation to IL-7 receptor in myeloid cells of rheumatoid arthritis and collagen-induced arthritis. J Immunol, 2013-04-19;190(10):5256-66. 2013-04-19 [PMID: 23606539] (Bioassay, Human)
• Zizzo G, Cohen P IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids. J Immunol, 2013-04-17;190(10):5237-46. 2013-04-17 [PMID: 23596310] (Bioassay, Human)
• Rosen L, Freeman A, Yang L, Jutivorakool K, Olivier K, Angkasekwinai N, Suputtamongkol Y, Bennett J, Pyrgos V, Williamson P, Ding L, Holland S, Browne S Anti-GM-CSF autoantibodies in patients with cryptococcal meningitis. J Immunol, 2013-03-18;190(8):3959-66. 2013-03-18 [PMID: 23509356] (Bioassay, Human)
• Johnston A, Xing X, Swindell W, Kochkodan J, Riblett M, Nair R, Stuart P, Ding J, Voorhees J, Elder J, Gudjonsson J Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis. Hum Mol Genet, 2013-01-31;22(9):1807-15. 2013-01-31 [PMID: 23376980] (Bioassay, Human)
• Truchetet M, Brembilla N, Montanari E, Lonati P, Raschi E, Zeni S, Fontao L, Meroni P, Chizzolini C Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement. Arthritis Rheum, 2013-05-01;65(5):1347-56. 2013-05-01 [PMID: 23335253] (Bioassay, Human)
• Gordon K, Kochkodan J, Blatt H, Lin S, Kaplan N, Johnston A, Swindell W, Hoover P, Schlosser B, Elder J, Gudjonsson J, Getsios S Alteration of the EphA2/Ephrin-A signaling axis in psoriatic epidermis. J Invest Dermatol, 2012-11-29;133(3):712-22. 2012-11-29 [PMID: 23190894] (Bioassay, Human)
• Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. PLoS Biol, 2012-09-25;10(9):e1001395. 2012-09-25 [PMID: 23055831] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Ramirez-Carrozzi V, Sambandam A, Luis E IL-17C regulates the innate immune function of epithelial cells in an autocrine manner. Nat. Immunol., 2011-10-12;12(12):1159-66. 2011-10-12 [PMID: 21993848] (Bioassay, Human)
• Liu Y, Mei J, Gonzales L, Yang G, Dai N, Wang P, Zhang P, Favara M, Malcolm KC, Guttentag S, Worthen GS IL-17A and TNF-alpha Exert Synergistic Effects on Expression of CXCL5 by Alveolar Type II Cells In Vivo and In Vitro. J. Immunol., 2011-01-31;186(5):3197-205. 2011-01-31 [PMID: 21282514] (Bioassay, Human)
• Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression. J. Immunol., 2011-01-17;186(4):2613-22. 2011-01-17 [PMID: 21242515] (Bioassay, Human)
• Gudjonsson JE, Johnston A, Stoll SW Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 2010-04-08;130(7):1849-59. 2010-04-08 [PMID: 20376066] (Bioassay, Human)
• Shan M, Cheng HF, Song LZ Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema. Sci Transl Med, 2009-10-28;1(4):4ra10. 2009-10-28 [PMID: 20368170] (Bioassay, Human)
• Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. J. Immunol., 2010-03-31;184(9):4880-8. 2010-03-31 [PMID: 20357258] (Bioassay, Human)
• Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis. J. Immunol., 2010-03-24;184(0):5263-70. 2010-03-24 [PMID: 20335534] (Bioassay, Human)
• Kisand K, Boe Wolff AS, Podkrajsek KT, Tserel L, Link M, Kisand KV, Ersvaer E, Perheentupa J, Erichsen MM, Bratanic N, Meloni A, Cetani F, Perniola R, Ergun-Longmire B, Maclaren N, Krohn KJ, Pura M, Schalke B, Strobel P, Leite MI, Battelino T, Husebye ES, Peterson P, Willcox N, Meager A Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines. J. Exp. Med., 2010-02-01;207(2):299-308. 2010-02-01 [PMID: 20123959] (ELISA (Capture), Human)
• Nishida A, Andoh A, Imaeda H, Inatomi O, Shiomi H, Fujiyama Y Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts. Gut, 2009-12-08;59(4):531-41. 2009-12-08 [PMID: 19996325] (Bioassay, Human)
• Xu G, Zhang L, Wang DY, Xu R, Liu Z, Han DM, Wang XD, Zuo KJ, Li HB Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells. Allergy, 2009-11-25;65(5):581-9. 2009-11-25 [PMID: 19968632] (Bioassay, Human)
• Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients. Immunol. Lett., 2009-11-04;127(2):150-6. 2009-11-04 [PMID: 19895848] (ELISA (Standard))
• Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Docke WD, Asadullah K, Volk HD, Sterry W, Sabat R The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis. Eur. J. Immunol., 2009-12-01;39(12):3570-81. 2009-12-01 [PMID: 19830738] (Bioassay, Human)
• Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur. J. Immunol., 2009-10-01;39(10):2779-88. 2009-10-01 [PMID: 19731362] (Bioassay, Human)
• Nishida A, Andoh A, Inatomi O, Fujiyama Y Interleukin-32 expression in the pancreas. J. Biol. Chem., 2009-04-21;284(26):17868-76. 2009-04-21 [PMID: 19386602] (Bioassay, Human)
• Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int. J. Cancer, 2009-07-15;125(2):367-73. 2009-07-15 [PMID: 19378341] (Bioassay, Human)
• Shahrara S, Pickens SR, Dorfleutner A, Pope RM IL-17 induces monocyte migration in rheumatoid arthritis. J. Immunol., 2009-03-15;182(6):3884-91. 2009-03-15 [PMID: 19265168] (Bioassay, Human)
• Amiable N, Tat SK, Lajeunesse D, Duval N, Pelletier JP, Martel-Pelletier J, Boileau C Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts. Bone, 2009-03-02;44(6):1143-50. 2009-03-02 [PMID: 19264156] (Bioassay, Human)
• Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 2009-03-02;119(10):1424-32. 2009-03-02 [PMID: 19255340] (Bioassay, Human)
• Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes. J. Immunol., 2009-03-01;182(0):3112-20. 2009-03-01 [PMID: 19234208] (Bioassay, Human)
• Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response. J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4. 2008-12-03 [PMID: 19056110] (Bioassay, Human)
• Kinoshita H, Takai T, Le TA, Kamijo S, Wang XL, Ushio H, Hara M, Kawasaki J, Vu AT, Ogawa T, Gunawan H, Ikeda S, Okumura K, Ogawa H Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J. Allergy Clin. Immunol., 2008-12-03;123(1):179-86. 2008-12-03 [PMID: 19056108] (Bioassay, Human)
• Kwan Tat S, Pelletier JP, Amiable N, Boileau C, Lajeunesse D, Duval N, Martel-Pelletier J Activation of the receptor EphB4 by its specific ligand ephrin B2 in human osteoarthritic subchondral bone osteoblasts. Arthritis Rheum., 2008-12-01;58(12):3820-30. 2008-12-01 [PMID: 19035475] (Bioassay, Human)
• Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis. J. Immunol., 2008-11-15;181(10):7420-7. 2008-11-15 [PMID: 18981165] (Bioassay, Human)
• Rahmoun M, Moles JP, Pedretti N, Mathieu M, Fremaux I, Raison-Peyron N, Lecron JC, Yssel H, Pene J Cytokine-induced CEACAM1 expression on keratinocytes is characteristic for psoriatic skin and contributes to a prolonged lifespan of neutrophils. J. Invest. Dermatol., 2008-10-09;129(3):671-81. 2008-10-09 [PMID: 18843289] (Bioassay, Human)
• Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, Ohba M, Sueki H, Kokubu F, Hizawa N, Adachi M, Huang SK, Iijima M Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis. J. Invest. Dermatol., 2008-10-02;129(3):650-6. 2008-10-02 [PMID: 18830271] (Bioassay, Human)
• Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J. Immunol., 2008-09-15;181(6):4299-310. 2008-09-15 [PMID: 18768888] (Bioassay, Human)
• Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J. Immunol., 2008-08-15;181(4):2799-805. 2008-08-15 [PMID: 18684971] (Bioassay, Surface Plasmon Resonance, Human)
• Kato A, Peters A, Suh L, Carter R, Harris KE, Chandra R, Conley D, Grammer LC, Kern R, Schleimer RP Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps. J. Allergy Clin. Immunol., 2008-04-14;121(6):1385-92, 1392. 2008-04-14 [PMID: 18410958] (Bioassay, Human)
• Aujla SJ, Chan YR, Zheng M, Fei M, Askew DJ, Pociask DA, Reinhart TA, McAllister F, Edeal J, Gaus K, Husain S, Kreindler JL, Dubin PJ, Pilewski JM, Myerburg MM, Mason CA, Iwakura Y, Kolls JK IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia. Nat. Med., 2008-02-10;14(3):275-81. 2008-02-10 [PMID: 18264110] (Bioassay, Human)
• Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood. J. Immunol., 2008-01-01;180(1):655-63. 2008-01-01 [PMID: 18097068] (Bioassay, Human)
• Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Ultrasensitive flow-based immunoassays using single-molecule counting. Clin. Chem., 2007-09-21;53(11):1990-5. 2007-09-21 [PMID: 17890441] (ELISA (Standard))
• Shioya M, Nishida A, Yagi Y, Ogawa A, Tsujikawa T, Kim-Mitsuyama S, Takayanagi A, Shimizu N, Fujiyama Y, Andoh A Epithelial overexpression of interleukin-32alpha in inflammatory bowel disease. Clin. Exp. Immunol., 2007-06-22;149(3):480-6. 2007-06-22 [PMID: 17590175] (Bioassay, Human)
• Wolk K, Witte E, Hoffmann U, Doecke WD, Endesfelder S, Asadullah K, Sterry W, Volk HD, Wittig BM, Sabat R IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn&apos;s disease. J. Immunol., 2007-05-01;178(9):5973-81. 2007-05-01 [PMID: 17442982] (Bioassay, Human)
• Brechter AB, Lerner UH Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression. Arthritis Rheum., 2007-03-01;56(3):910-23. 2007-03-01 [PMID: 17328065] (Bioassay, Human)
• Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum. Kidney Int., 2007-01-17;71(6):514-25. 2007-01-17 [PMID: 17228364] (Bioassay, Human)
• Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Arthritis Rheum., 2006-07-01;54(7):2096-108. 2006-07-01 [PMID: 16802344] (Bioassay, Human)
• Vaknin-Dembinsky A, Balashov K, Weiner HL IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production. J. Immunol., 2006-06-15;176(12):7768-74. 2006-06-15 [PMID: 16751425] (ELISA (Specificity Testing))
• Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J. Immunol., 2005-11-01;175(9):6177-89. 2005-11-01 [PMID: 16237115] (Bioassay, Human)

FAQs for IL-17/IL-17A (317-ILB). (Showing 1 - 2 of 2 FAQs).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: IL17A
• Uniprot:
  • Q16552()