Recombinant Human IL-15 Protein, CF

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1 µg/lane of Recombinant Human IL-15 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 10 kDa.
Recombinant Human IL-15 (Catalog #247-ILB/CF) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.3-2.6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Recombinant Human IL-15 Protein, CF Summary

Accession #
N-terminal Sequence
Asn49
Protein/Peptide Type
Recombinant Proteins
Gene
IL15

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
10 kDa, reducing conditions
Publications
Read Publications using
247-ILB/CF in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-15 Protein, CF

  • IL15
  • IL-15
  • IL-15MGC9721
  • interleukin 15
  • interleukin-15

Background

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature human IL-15 protein shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of Interleukin 15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). The IL-15 protein binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (7). This enables cells to respond to Interleukin 15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 protein in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells (12-16). An important component of these functions is the ability of IL-15 to induce dendritic cell differentiation and inflammatory activation (11, 14). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells (17). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (18).

  1. De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  2. Grabstein, K. et al. (1994) Science 264:965.
  3. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  4. Giri, J.G. et al. (1995) EMBO J. 14:3654.
  5. Giri, J. et al. (1994) EMBO J. 13:2822.
  6. Dubois, S. et al. (2002) Immunity 17:537.
  7. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
  8. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  9. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  10. Bergamaschi, C. et al. (2012) Blood 120:e1.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  12. Mortier, E. et al. (2003) J. Exp. Med. 205:1213.
  13. Gordy, L.E. et al. (2011) J. Immunol. 187:6335.
  14. Harris, K.M. (2011) J. Leukoc. Biol. 90:727.
  15. Xia, J. et al. (2010) Clin. Immunol. 134:130.
  16. Schluns, K.S. et al. (2002) J. Immunol. 168:4827.
  17. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529.
  18. Barra, N.G. et al. (2010) Obesity 18:1601.

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Publications for IL-15 (247-ILB/CF)(147)

We have publications tested in 7 confirmed species: Human, Mouse, Bovine, CD45.1 P14TCM, N/A, Primate - Macaca mulatta (Rhesus Macaque), Xenograft.

We have publications tested in 6 applications: Bioassay, CAR-T (Bioassay), Cell Culture, ELISA (Standard), In Vivo, Western Blot.


Filter By Application
Bioassay
(120)
CAR-T (Bioassay)
(3)
Cell Culture
(16)
ELISA (Standard)
(3)
In Vivo
(4)
Western Blot
(1)
All Applications
Filter By Species
Human
(127)
Mouse
(11)
Bovine
(1)
CD45.1 P14TCM
(1)
N/A
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 147. Show All 147 Publications.
Publications using 247-ILB/CF Applications Species
P Sekhri, DK Ledezma, A Shukla, EE Sweeney, R Fernandes The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma Cells Cancers, 2022;14(6):. 2022 [PMID: 35326601] (Cell Culture, Human) Cell Culture Human
HC Liang, M Costanza, N Prutsch, MW Zimmerman, E Gurnhofer, IA Montes-Moj, BJ Abraham, N Prokoph, S Stoiber, S Tangermann, C Lobello, J Oppelt, I Anagnostop, T Hielscher, S Pervez, W Klapper, F Zammarchi, DA Silva, KC Garcia, D Baker, M Janz, N Schleussne, F Fend, Š Pospíšilov, A Janiková, J Wallwitz, D Stoiber, I Simonitsch, L Cerroni, S Pileri, L de Leval, D Sibon, V Fataccioli, P Gaulard, C Assaf, F Knörr, C Damm-Welk, W Woessmann, SD Turner, AT Look, S Mathas, L Kenner, O Merkel Super-enhancer-based identification of a BATF3/IL-2R-module reveals vulnerabilities in anaplastic large cell lymphoma Nature Communications, 2021;12(1):5577. 2021 [PMID: 34552066] (Cell Culture, Human) Cell Culture Human
AS Bear, T Blanchard, J Cesare, MJ Ford, LP Richman, C Xu, ML Baroja, S McCuaig, C Costeas, K Gabunia, J Scholler, AD Posey, MH O'Hara, A Smole, DJ Powell, BA Garcia, RH Vonderheid, GP Linette, BM Carreno Biochemical and functional characterization of mutant KRAS epitopes validates this oncoprotein for immunological targeting Nature Communications, 2021;12(1):4365. 2021 [PMID: 34272369] (Bioassay, Human) Bioassay Human
F Hasan, Y Chiu, RM Shaw, J Wang, C Yee Hypoxia acts as an environmental cue for the human tissue-resident memory T cell differentiation program JCI Insight, 2021;6(10):. 2021 [PMID: 34027895] (Bioassay, Human) Bioassay Human
TR Emmons, T Giridharan, KL Singel, ANH Khan, J Ricciuti, K Howard, SL Silva-Del, IL Debreceni, CEM Aarts, MC Brouwer, S Suzuki, TW Kuijpers, I Jongerius, LH Allen, VP Ferreira, A Schubart, H Sellner, J Eder, SM Holland, S Ram, JA Lederer, KH Eng, KB Moysich, K Odunsi, MB Yaffe, E Zsiros, BH Segal Mechanisms Driving Neutrophil-Induced T-cell Immunoparalysis in Ovarian Cancer Cancer Immunology Research, 2021;9(7):790-810. 2021 [PMID: 33990375] (Bioassay, Human) Bioassay Human
AM Farhat, AC Weiner, C Posner, ZS Kim, B Orcutt-Jah, SM Carlson, AS Meyer Modeling cell-specific dynamics and regulation of the common gamma chain cytokines Cell Reports, 2021;35(4):109044. 2021 [PMID: 33910015] (Bioassay, Human) Bioassay Human
S Jung, E Appleton, M Ali, GM Church, A Del Sol A computer-guided design tool to increase the efficiency of cellular conversions Nature Communications, 2021;12(1):1659. 2021 [PMID: 33712564] (Bioassay, Human) Bioassay Human
AK Singh, E Schetzen, SK Yadav, EB Lustig, WH Liu, RK Yadav, RP Gale, K McGinnis, Y Reisner Correction of murine sickle cell disease by allogeneic haematopoietic cell transplantation with anti-3rd party veto cells Bone marrow transplantation, 2021;0(0):. 2021 [PMID: 33658643] (Bioassay, Mouse) Bioassay Mouse
N Gimenez, R Tripathi, A Giró, L Rosich, M López-Guer, I López-Orej, H Playa-Albi, F Arenas, JM Mas, P Pérez-Galá, J Delgado, E Campo, J Farrés, D Colomer Systems biology drug screening identifies statins as enhancers of current therapies in chronic lymphocytic leukemia Scientific Reports, 2020;10(1):22153. 2020 [PMID: 33335123] (Cell Culture, Human) Cell Culture Human
KK Naidoo, ZB Shangase, T Rashid, A Ngubane, N Ismail, T Ndung'u, CF Thobakgale The DARC-null trait is associated with moderate modulation of NK cell profiles and unaltered cytolytic T cell profiles in black South Africans PLoS ONE, 2020;15(11):e0242448. 2020 [PMID: 33211774] (Bioassay, Human) Bioassay Human
Show All 147 Publications.

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Bioinformatics

Gene Symbol IL15
Uniprot