Recombinant Mouse IL-15R alpha Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse IL-15R alpha Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells. Ruchatz, H. et al. (1998) J. Immunol. 160:5654. The ED50 for this effect is 0.1-0.5 ng/mL in the presence of 0.1 ng/mL recombinant human IL-15.
Source
Mouse myeloma cell line, NS0-derived mouse IL-15 R alpha protein
Mouse IL-15 R alpha
(Gly33 - Lys205)
Accession # Q60819
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Contains a small amount (10%) of free IL-15 R alpha and Fc generated by proteolytic cleavage.
Accession #
N-terminal Sequence
Gly33
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il15ra
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
44.9 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-90 kDa, 42 kDa and 35 kDa, reducing conditions
Publications
Read Publications using
551-MR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-15R alpha Fc Chimera Protein, CF

  • CD215 antigen
  • CD215
  • IL15 R alpha
  • IL-15 R alpha
  • IL-15 receptor subunit alpha
  • IL-15R alpha
  • IL15RA
  • IL-15Ra
  • IL-15R-alpha
  • interleukin 15 receptor, alpha
  • interleukin-15 receptor subunit alpha
  • MGC104179

Background

Interleukin 15 Receptor alpha (IL 15 R alpha ), also known as CD215, is a widely expressed 60 kDa transmembrane glycoprotein that plays an important role in the homeostasis and activation of NK cells and CD8+ memory T cells and participates in the development and function of many other hematopoietic cell types and non‑immune cell types (1 ‑ 3). Mature mouse IL‑15 R alpha consists of a 173 aa extracellular domain (ECD) containing one N‑linked glycosylation site, a 21 aa transmembrane segment, and a 37 aa cytoplasmic tail (4). Within the ECD, mouse IL‑15 R alpha shares 59% and 89% aa sequence identity with human and rat IL‑15 R alpha, respectively. Alternate splicing of mouse IL‑15 R alpha generates additional isoforms with an N‑terminal truncation or variable length deletions in the ECD. IL‑15 R alpha binds to Interleukin‑15 with high affinity (4). IL‑15 additionally interacts with lower affinity to a complex of IL‑2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL‑2 receptor complex (5, 6). The use of shared receptor components contributes to the overlapping biological effects of IL‑15 and IL‑2. The dominant mechanism of IL‑15 action is known as transpresentation in which IL‑15/IL‑15 R alpha complexes are expressed on the surface of one cell and interact with complexes of IL‑2 R beta / gamma c on adjacent cells (7). This enables cells to respond to IL‑15 even if they do not express IL‑15 R alpha (8 ‑ 10). IL‑15/IL‑15 R alpha complexes can transmit reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL‑15/IL‑15 R alpha expressing cells (11, 12). Shed soluble forms of IL‑15 R alpha retain the ability to bind tightly to IL‑15 and can inhibit IL‑15 bioactivity (4, 13, 14).

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Di Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  3. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  4. Giri, J.G. et al. (1995) EMBO 14:3654.
  5. Grabstein, K. et al. (1994) Science 264:965.
  6. Giri, J. et al. (1994) EMBO J. 13:2822.
  7. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  8. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  9. Dubois, S. et al. (2002) Immunity 17:537.
  10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  12. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
  13. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  14. Mortier, E. et al. (2004) J. Immunol. 173:1681.

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Publications for IL-15R alpha (551-MR)(34)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 7 applications: Bioassay, Enzyme Assay, In Vivo, In vivo, Size Exclusion Chromatography, Stimulation, Surface Plasmon Resonance.


Filter By Application
Bioassay
(5)
Enzyme Assay
(1)
In Vivo
(24)
In vivo
(1)
Size Exclusion Chromatography
(1)
Stimulation
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Mouse
(33)
Rat
(1)
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using 551-MR Applications Species
M Yu, H Pan, N Che, L Li, C Wang, Y Wang, G Ma, M Qian, J Liu, M Zheng, H Xie, L Ling, Y Zhao, X Guan, Q Ding, W Zhou, S Wang Microwave ablation of primary breast cancer inhibits metastatic progression in model mice via activation of natural killer cells Cell. Mol. Immunol., 2020;0(0):. 2020 [PMID: 32385362] (In Vivo, Mouse) In Vivo Mouse
B Lucas, AJ White, EJ Cosway, SM Parnell, KD James, ND Jones, I Ohigashi, Y Takahama, WE Jenkinson, G Anderson Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells Nat Commun, 2020;11(1):2198. 2020 [PMID: 32366944] (In Vivo, Mouse) In Vivo Mouse
AS Schmid, D Neri Design and characterisation of a novel interleukin-15 receptor alpha fusion protein and analysis of interleukin-15 complexation PLoS ONE, 2019;14(7):e0219313. 2019 [PMID: 31348785] (Size Exclusion Chromatography, Mouse) Size Exclusion Chromatography Mouse
N Simonovi?, A Witalisz-S, K Meissl, C Lassnig, U Reichart, T Kolbe, M Farlik, C Bock, V Sexl, M Müller, B Strobl NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity J. Immunol., 2019;0(0):. 2019 [PMID: 30718299] (In Vivo, Mouse) In Vivo Mouse
KS Burrack, MA Huggins, E Taras, P Dougherty, CM Henzler, R Yang, S Alter, EK Jeng, HC Wong, M Felices, F Cichocki, JS Miller, GT Hart, AJ Johnson, SC Jameson, SE Hamilton Interleukin-15 Complex Treatment Protects Mice from Cerebral Malaria by Inducing Interleukin-10-Producing Natural Killer Cells Immunity, 2018;0(0):. 2018 [PMID: 29625893] (In Vivo, Mouse) In Vivo Mouse
SMS Islam, B Choi, J Choi, ES Lee, S Sohn Frequencies of IL-15R?+ cells in patients with Beh�et&#039;s disease and the effects of overexpressing IL-15R?+ on disease symptoms in mice Cytokine, 2018;0(0):. 2018 [PMID: 29396044] (In Vivo, Mouse) In Vivo Mouse
JH DeLong, AO Hall, C Konradt, GM Coppock, J Park, G Harms Prit, CA Hunter Cytokine- and TCR-Mediated Regulation of T Cell Expression of Ly6C and Sca-1 J. Immunol., 2018;0(0):. 2018 [PMID: 29358280] (Bioassay, Mouse) Bioassay Mouse
RT Sowell, JW Goldufsky, M Rogozinska, Z Quiles, Y Cao, EF Castillo, A Finnegan, AL Marzo IL-15 Complexes Induce Migration of Resting Memory CD8 T Cells into Mucosal Tissues J. Immunol., 2017;0(0):. 2017 [PMID: 28814601] (In Vivo, Mouse) In Vivo Mouse
H Kubo, S Mensurado, N Goncalves-, K Serre, B Silva-Sant Primary tumors limit metastasis formation through induction of IL15-mediated crosstalk between patrolling monocytes and NK cells Cancer Immunol Res, 2017;0(0):. 2017 [PMID: 28811289] (Bioassay, Mouse) Bioassay Mouse
I Kavazovi?, M Lenarti?, V Jelen?i?, S Jurkovi?, NA Lemmermann, S Jonji?, B Poli?, FM Wensveen NKG2D stimulation of CD8+ T cells during priming promotes their capacity to produce cytokines in response to viral infection in mice Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 28378389] (In Vivo, Mouse) In Vivo Mouse
Show All 34 Publications.

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Bioinformatics

Gene Symbol Il15ra
Uniprot