Recombinant Human IL-10 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-10 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.15-0.75 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-10 protein
Ser19-Asn178
Accession #
N-terminal Sequence
Ser19
Structure / Form
Noncovalently-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
IL10
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
217-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-10 Protein

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL‑10 family of alpha ‑helical cytokines that also includes
IL‑19, IL‑20, IL‑22, IL‑24, and IL‑26/AK155 (1, 2). IL‑10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature human IL‑10 shares 72%‑86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL‑10. Whereas human IL‑10 is active on mouse cells, mouse IL‑10 does not act on human cells (6, 7). IL‑10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL‑10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL‑10 R beta does not bind IL‑10 by itself but is required for signal transduction (1). IL‑10 R beta also associates with IL‑20 R alpha, IL‑22R alpha, or IL‑28 R alpha to form the receptor complexes for IL‑22, IL‑26, IL‑28, and IL‑29
(11‑13). IL‑10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14‑16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).
  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. Sabat, R. et al. (2010) Cytokine Growth Factor Rev. 21:331.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  9. Syto, R. et al. (1998) Biochemistry 37:16943.
  10. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  11. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  12. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  13. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  14. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  15. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  16. Blackburn, S.D. and E.J. Wherry (2007)Trends Microbiol. 15:143.

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217-IL
Species: Hu
Applications: Bioactivity

Publications for IL-10 (217-IL)(89)

We have publications tested in 5 confirmed species: Human, Mouse, Hamster, N/A, Primate - Papio anubis (Olive Baboon).

We have publications tested in 4 applications: Bioassay, Control, ELISA (Standard), Standard.


Filter By Application
Bioassay
(80)
Control
(1)
ELISA (Standard)
(5)
Standard
(1)
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Filter By Species
Human
(76)
Mouse
(2)
Hamster
(1)
N/A
(7)
Primate - Papio anubis (Olive Baboon)
(1)
All Species
Showing Publications 1 - 10 of 89. Show All 89 Publications.
Publications using 217-IL Applications Species
Y Ye, M Liu, L Tang, F Du, Y Liu, P Hao, Q Fu, Q Guo, Q Yan, X Zhang, C Bao Iguratimod represses B cell terminal differentiation linked with the inhibition of PKC/EGR1 axis Arthritis Res. Ther., 2019;21(1):92. 2019 [PMID: 30971291] (Bioassay, Human) Bioassay Human
BA Kane, H An, P Rajasekari, HP McNeil, K Bryant, N Tedla Differential expression and regulation of the non-integrin 37/67-kDa laminin receptor on peripheral blood leukocytes of healthy individuals and patients with rheumatoid arthritis Sci Rep, 2019;9(1):1149. 2019 [PMID: 30718719] (Bioassay, Human) Bioassay Human
F Charbit-He, B Bègue, A Sierra, S Hanein, MC Stolzenber, Z Li, S Pellegrini, N Garcelon, M Jeanpierre, B Neven, I Loge, C Picard, J Rosain, J Bustamante, M Le Lorc'h, B Pigneur, A Fernandes,, F Rieux-Lauc, J Amil Dias, FM Ruemmele, N Cerf-Bensu Copy number variations and founder effect underlying complete IL-10R? deficiency in Portuguese kindreds PLoS ONE, 2018;13(10):e0205826. 2018 [PMID: 30365510] (Bioassay, Human) Bioassay Human
AS Hansen, M Biltoft, B Bundgaard, AB Bohn, BK Møller, P Höllsberg CD46 activation induces distinct CXCL-10 response in monocytes and monocyte-derived dendritic cells Cytokine, 2018;0(0):. 2018 [PMID: 29980471] (Bioassay, Human) Bioassay Human
EW Kim, RMB Teles, S Haile, PT Liu, RL Modlin Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae PLoS Negl Trop Dis, 2018;12(7):e0006608. 2018 [PMID: 29965969] (Bioassay, Human) Bioassay Human
SL Jaslow, KD Gibbs, WF Fricke, L Wang, KJ Pittman, MK Mammel, JT Thaden, VG Fowler, GE Hammer, JR Elfenbein, DC Ko Salmonella Activation of STAT3 Signaling by SarA Effector Promotes Intracellular Replication and Production of IL-10 Cell Rep, 2018;23(12):3525-3536. 2018 [PMID: 29924996] (Bioassay, Human) Bioassay Human
A Palazzo, S Herter, L Grosmaire, R Jones, CR Frey, F Limani, M Bacac, P Umana, RJ Oldham, MJE Marshall, KL Cox, AH Turaj, MS Cragg, C Klein, MJ Carter, S Tannheimer The PI3K?-Selective Inhibitor Idelalisib Minimally Interferes with Immune Effector Function Mediated by Rituximab or Obinutuzumab and Significantly Augments B Cell Depletion In Vivo J. Immunol., 2018;0(0):. 2018 [PMID: 29453281] (Bioassay, Human) Bioassay Human
U Gopinathan, KR Redalen, AM Trøseid, P Kierulf, P Brandtzaeg, AH Ree, JP Berg, R Øvstebø Large-scale reduction of tyrosine kinase activities in human monocytes stimulated in vitro with N. meningitidis PLoS ONE, 2018;13(1):e0181912. 2018 [PMID: 29357362] (Bioassay, Human) Bioassay Human
RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain PLoS ONE, 2017;12(10):e0186317. 2017 [PMID: 29016674] (Bioassay, Mouse) Bioassay Mouse
X Li, P Lu, B Li, W Zhang, R Yang, Y Chu, K Luo Interleukin 2 and interleukin 10 function synergistically to promote CD8(+) T cell cytotoxicity, which is suppressed by regulatory T cells in breast cancer Int. J. Biochem. Cell Biol, 2017;0(0):. 2017 [PMID: 28274688] (Bioassay, Human) Bioassay Human
Show All 89 Publications.

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Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Bioinformatics

Gene Symbol IL10
Uniprot