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Recombinant Human IL-1 beta/IL-1F2 Protein, CF

Images

 
1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human IL-1 beta/IL-1F2 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is <12 pg/mL.
The specific activity of recombinant human IL-1 beta is approximately 1.4 x 105 IU/μg, which is calibrated against recombinant human IL-1 beta WHO International Standard (NIBSC code: 86/680).   Specific activity is for reference purposes only and is not routinely tested.  
Source
E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269
Accession #
N-terminal Sequence
Ala117 & Pro118
Protein/Peptide Type
Recombinant Proteins
Gene
IL1B
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
201-LB/CF in the following applications:

Packaging, Storage & Formulations

Storage


Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50-200 μg/mL in sterile PBS.

Notes

This product is covered by U.S. patent: 5,681,933.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 beta/IL-1F2 Protein, CF

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta .

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. March, C.J. et al. (1985) Nature 315:641.
  6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

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201-LB/CF
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Publications for IL-1 beta/IL-1F2 (201-LB/CF)(483)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, N/A, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee), Rabbit.

We have publications tested in 9 applications: Activation, Binding Assay, Bioassay, Cell Culture, ELISA (Standard), In Vivo, Standard, Stimulation, Western Blot Control.


Filter By Application
Activation
(1)
Binding Assay
(1)
Bioassay
(405)
Cell Culture
(37)
ELISA (Standard)
(11)
In Vivo
(8)
Standard
(1)
Stimulation
(2)
Western Blot Control
(1)
All Applications
Filter By Species
Human
(413)
Mouse
(19)
Rat
(10)
Bovine
(4)
Canine
(3)
Guinea Pig
(1)
Hamster
(1)
N/A
(12)
Porcine
(3)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Primate - Pan troglodytes (Chimpanzee)
(1)
Rabbit
(4)
All Species
Showing Publications 1 - 10 of 483. Show All 483 Publications.
Publications using 201-LB/CF Applications Species
PM Varghese, U Kishore, R Rajkumari Human C1q Regulates Influenza A Virus Infection and Inflammatory Response via Its Globular Domain International Journal of Molecular Sciences, 2022;23(6):. 2022 [PMID: 35328462] (Bioassay, Human) Bioassay Human
P Sekhri, DK Ledezma, A Shukla, EE Sweeney, R Fernandes The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma Cells Cancers, 2022;14(6):. 2022 [PMID: 35326601] (Cell Culture, Human) Cell Culture Human
JFS Pereira, C Bessa, P Matos, P Jordan Pro-Inflammatory Cytokines Trigger the Overexpression of Tumour-Related Splice Variant RAC1B in Polarized Colorectal Cells Cancers, 2022;14(6):. 2022 [PMID: 35326545] (Cell Culture, Human) Cell Culture Human
S Ma, SA Patel, Y Abe, N Chen, PR Patel, BS Cho, N Abbasi, S Zeng, B Schnabl, JT Chang, WJM Huang RORgammat phosphorylation protects against T�cell-mediated inflammation Cell Reports, 2022;38(11):110520. 2022 [PMID: 35294872] (Cell Culture, Human) Cell Culture Human
JG Miranda, WE Schleicher, KL Wells, DG Ramirez, SP Landgrave, RK Benninger Dynamic changes in beta-cell [Ca2+] regulates NFAT activation, gene transcription and islet gap junction communication Oncogene, 2021;0(0):101430. 2021 [PMID: 34979329] (Bioassay, Human) Bioassay Human
A Tosi, L Nardinocch, ML Carbone, L Capriotti, E Pagani, S Mastroeni, C Fortes, F Scopelliti, C Cattani, F Passarelli, A Rosato, S D'Atri, CM Failla, A Cavani Reduced Interleukin-17-Expressing Cells in Cutaneous Melanoma Biomedicines, 2021;9(12):. 2021 [PMID: 34944746] (Bioassay, Human) Bioassay Human
PM Panipinto, AK Singh, FS Shaikh, RJ Siegel, M Chourasia, S Ahmed Takinib Inhibits Inflammation in Human Rheumatoid Arthritis Synovial Fibroblasts by Targeting the Janus Kinase-Signal Transducer and Activator of Transcription 3 (JAK/STAT3) Pathway International Journal of Molecular Sciences, 2021;22(22):. 2021 [PMID: 34830460] (Bioassay, Human) Bioassay Human
A Fu, L van Rooyen, L Evans, N Armstrong, D Avizonis, T Kin, GH Bird, A Reddy, ET Chouchani, M Liesa-Roig, LD Walensky, AMJ Shapiro, NN Danial Glucose metabolism and pyruvate carboxylase enhance glutathione synthesis and restrict oxidative stress in pancreatic islets Cell Reports, 2021;37(8):110037. 2021 [PMID: 34818536] (Bioassay, Human) Bioassay Human
SA Saenz, A Local, T Carr, A Shakya, S Koul, H Hu, L Chourb, J Stedman, J Malley, LA D'Agostino, V Shanmugasu, J Malona, CE Schwartz, L Beebe, M Clements, G Rajaraman, J Cho, L Jiang, A Dubrovskiy, M Kreilein, R Shimanovic, LG Hamann, L Escoubet, JM Ellis Small molecule allosteric inhibitors of RORgammat block Th17-dependent inflammation and associated gene expression in vivo PLoS ONE, 2021;16(11):e0248034. 2021 [PMID: 34752458] (Bioassay, Human) Bioassay Human
C Xu, J Tang, Q Yang, H Zhao, Y Liu, J Cao, Y Zhou, X Chen, J Chen Histone deacetylase 5 deacetylates the phosphatase PP2A for positively regulating NF-kappaB signaling The Journal of Biological Chemistry, 2021;0(0):101380. 2021 [PMID: 34740611] (Bioassay, Human) Bioassay Human
Show All 483 Publications.

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Bioinformatics

Gene Symbol IL1B
Uniprot