Recombinant Human IL-1 beta/IL-1F2 Protein, CF

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Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.
1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human IL-1 beta/IL-1F2 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is <12 pg/mL.
The specific activity of recombinant human IL-1 beta is approximately 1.4 x 105 IU/μg, which is calibrated against recombinant human IL-1 beta WHO International Standard (NIBSC code: 86/680).
Source
E. coli-derived
Ala117-Ser269
Accession #
N-terminal Sequence
Ala117 & Pro118
Protein/Peptide Type
Recombinant Proteins
Gene
IL1B
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
201-LB/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is covered by U.S. patent: 5,681,933.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 beta/IL-1F2 Protein, CF

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

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201-LB/CF
Species: Hu
Applications: BA

Publications for IL-1 beta/IL-1F2 (201-LB/CF)(291)

We have publications tested in 13 confirmed species: Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, N/A, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee), Rabbit.

We have publications tested in 7 applications: Binding Assay, Bioassay, Bioassay Standard, ELISA Standard, In Vivo, Standard, cell culture.


Filter By Application
Binding Assay
(1)
Bioassay
(260)
Bioassay Standard
(1)
ELISA Standard
(11)
In Vivo
(8)
Standard
(1)
cell culture
(3)
All Applications
Filter By Species
Human
(238)
Mouse
(13)
Rat
(5)
Bovine
(3)
Canine
(1)
Guinea Pig
(1)
Hamster
(1)
N/A
(12)
Porcine
(2)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Primate - Pan troglodytes (Chimpanzee)
(1)
Rabbit
(3)
All Species
Showing Publications 1 - 10 of 291. Show All 291 Publications.
Publications using 201-LB/CF Applications Species
L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis Autophagy, 2017;0(0):0. 2017 [PMID: 28933598] (Bioassay, Human) Bioassay Human
N Akbar, JE Digby, TJ Cahill, AN Tavare, AL Corbin, S Saluja, S Dawkins, L Edgar, N Rawlings, K Ziberna, E McNeill,, E Johnson, AA Aljabali, RA Dragovic, M Rohling, TG Belgard, IA Udalova, DR Greaves, KM Channon, PR Riley, DC Anthony, RP Choudhury Endothelium-derived extracellular vesicles promote splenic monocyte mobilization in myocardial infarction JCI Insight, 2017;2(17):. 2017 [PMID: 28878126] (Bioassay, Human) Bioassay Human
A Steinert, I Linas, B Kaya, M Ibrahim, A Schlitzer, P Hruz, K Radulovic, L Terraccian, AJ Macpherson, JH Niess The Stimulation of Macrophages with TLR Ligands Supports Increased IL-19 Expression in Inflammatory Bowel Disease Patients and in Colitis Models J. Immunol., 2017;0(0):. 2017 [PMID: 28864472] (Bioassay, Mouse) Bioassay Mouse
L Ding, JA Buckwalter, JA Martin DAMPs Synergize with Cytokines or Fibronectin Fragment on Inducing Chondrolysis but Lose Effect When Acting Alone Mediators Inflamm., 2017;2017(0):2642549. 2017 [PMID: 28804219] (Bioassay, Bovine) Bioassay Bovine
TM Nordmann, E Dror, F Schulze, S Traub, E Berishvili, C Barbieux, M Böni-Schne, MY Donath The Role of Inflammation in ?-cell Dedifferentiation Sci Rep, 2017;7(1):6285. 2017 [PMID: 28740254] (Bioassay, Human) Bioassay Human
AE van der Wi, IMC Vogels, CJF van Noorde, I Klaassen, RO Schlingema TNF?-Induced Disruption of the Blood-Retinal Barrier In Vitro Is Regulated by Intracellular 3&#039;,5&#039;-Cyclic Adenosine Monophosphate Levels Invest. Ophthalmol. Vis. Sci., 2017;58(9):3496-3505. 2017 [PMID: 28715583] (Bioassay, Human) Bioassay Human
R Connor, LD Jones, X Qiu, J Thakar, SB Maggirwar Frontline Science: c-Myc regulates P-selectin glycoprotein ligand-1 expression in monocytes during HIV-1 infection J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28663244] (Bioassay, Human) Bioassay Human
L Šenolt, K Prajzlerov, H Hulejová, B Šumová, M Filková, D Veigl, K Pavelka, J Vencovský Interleukin-20 is triggered by TLR ligands and associates with disease activity in patients with rheumatoid arthritis Cytokine, 2017;97(0):187-192. 2017 [PMID: 28662439] (Bioassay, Human) Bioassay Human
J Ye, Y Wang, X Liu, L Li, A Opejin, EC Hsueh, H Luo, T Wang, D Hawiger, G Peng TLR7 Signaling Regulates Th17 Cells and Autoimmunity: Novel Potential for Autoimmune Therapy J. Immunol., 2017;0(0):. 2017 [PMID: 28652396] (Bioassay, Human) Bioassay Human
J Shen, C Wang, D Li, T Xu, J Myers, JM Ashton, T Wang, MJ Zuscik, A McAlinden, RJ O'Keefe DNA methyltransferase 3b regulates articular cartilage homeostasis by altering metabolism JCI Insight, 2017;2(12):. 2017 [PMID: 28614801] (Bioassay, Human) Bioassay Human
Show All 291 Publications.

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IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol IL1B
Entrez
Uniprot