Recombinant Human HSP70/HSPA1A Protein, CF

• Reactivity: Hu
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human HSP70/HSPA1A Protein, CF Summary

• Details of Functionality:

  Reaction conditions will need to be optimized for each specific application. We recommend an initial HSP70/HSPA1A concentration of 2-3 μM for in vitro use. IMPORTANT: HSP40/DNAJB1 (Catalog # AP-110), or another suitable co-chaperone, is required for HSP70/HSPA1A activity and should be used at a concentration that is equimolar to HSP70/HSPA1A.

• Source: E. coli-derived human HSP70/HSPA1A protein
  Met1 - Asp641
• Accession #: AAD21816.1
• Protein/Peptide Type: Recombinant Proteins
• Gene: HSPA1A
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, DTT.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HSP70/HSPA1A Protein, CF

• dnaK-type molecular chaperone HSP70-1
• FLJ54303
• FLJ54370
• FLJ54392
• FLJ54408
• FLJ75127
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1A
• heat shock 70kDa protein 1A
• heat shock-induced protein
• HSP70
• HSP70.1/HSP70.2
• HSP70-1
• HSP70-1/HSP70-2
• HSP70-1A
• HSP70I
• HSP72
• HSPA1
• HSPA1A
• HSPA1B

Background

Heat Shock 70kDa Protein (HSP70), also known as Heat Shock 70kDa Protein 1A (HSPA1A), is a 641 amino acid (aa) member of the HSP70 family of molecular chaperones with a predicted molecular weight of 70 kDa. Human HSP70/HSPA1A shares 95% and 97% aa sequence identity with the mouse and rat orthologs, respectively. It has an N-terminal nucleotide-binding domain, which contains ATPase activity, and a C-terminal substrate-binding domain (1). HSP70/HSPA1A promotes the proper folding of nascent polypeptides and assists in the refolding of denatured proteins (2). However, if either of these processes proceeds too slowly or fails, HSP70/HSPA1A can interact with the HSP40 co-chaperone protein and the CHIP/STUB1 Ubiquitin ligase (E3) to promote ubiquitination and degradation of the nascent polypeptide or denatured protein (3,4). HSP70/HSPA1A can be regulated post-translationally via multiple mechanisms, including phosphorylation, ubiquitination, and methylation (5-8). For example, unmethylated HSP70/HSPA1A localizes to the cytoplasm, but following methylation on Lys561 it is found only in the nucleus (8). Pathologically, HSP70/HSPA1A has been implicated in the promotion of multiple cancer types (8-10). Conversely, it is thought to protect against several neurodegenerative diseases that are caused by the accumulation of misfolded proteins (11,12).

1. Qi, R. et al. (2013) Nat. Struct. Mol. Biol. 20:900.
2. Kampinga, H.H. & E.A. Craig (2010) Nat. Rev. Mol. Cell Biol. 11:579.
3. McDonough, H. & C. Patterson (2003) Cell Stress Chaperones 8:303.
4. Donnelly, B.F. et al. (2013) J. Biol. Chem. 288:13124.
5. Muller, P. et al. (2012) Oncogene [Epub ahead of print].
6. Liu, M. et al. (2008) J. Biol. Chem. 283:35783.
7. Moore, D.J. et al. (2008) J. Neurochem. 105:1806.
8. Cho, H.S. et al. (2012) Nat. Commun. 3:1072.
9. Wu, F.H. et al. (2012) Cancer Lett. 317:157.
10. Gaudio, E. et al. (2013) Blood 121:351.
11. Miyata, Y. et al. (2011) Future Med. Chem. 3:1523.
12. Wang, A.M. et al. (2013) Nat. Chem. Biol. 9:112.

Publications for HSP70/HSPA1A (AP-100)(2)

Publications using AP-100

• K Kanno, K Shimizu, M Shinoda, M Hayashi, O Takeichi, K Iwata Role of macrophage-mediated Toll-like receptor 4-interleukin-1R signaling in ectopic tongue pain associated with tooth pulp inflammation J Neuroinflammation, 2020-10-21;17(1):312. 2020-10-21 [PMID: 33081813] (Bioassay, Rat)
• Ohara , Kinuyo, Shimizu , Kohei, Matsuura , Shingo, Ogiso , Bunnai, Omagari , Daisuke, Asano , Masatake, Tsuboi , Yoshiyuk, Shinoda , Masamich, Iwata , Koichi Toll-like receptor 4 signaling in trigeminal ganglion neurons contributes tongue-referred pain associated with tooth pulp inflammation. J Neuroinflammation, 2013-11-23;10(0):139. 2013-11-23 [PMID: 24267924] (IHC, Rat)

Bioinformatics

• Gene Symbol: HSPA1A
• Uniprot:
  • AAD21816.1()