Recombinant Human HGF (NS0-expressed) Protein

Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is equal to or less than 4.00 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human HGF (NS0-expressed) Protein Summary

• Details of Functionality: Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED₅₀ for this effect is equal to or less than 4.00 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human HGF protein
  Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
• Accession #: P14210.2
• N-terminal Sequence: alpha chain: No results obtained; Gln32 predicted
  beta chain: Val495
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: HGF
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: alpha chain: 53.7 kDa beta chain: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 71-85 kDa, non-reducing conditions
  56-65 kDa and 30-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HGF (NS0-expressed) Protein

• deafness, autosomal recessive 39
• DFNB39
• EC 3.4.21
• EC 3.4.21.7
• fibroblast-derived tumor cytotoxic factor
• F-TCF
• hepatocyte growth factor (hepapoietin A; scatter factor)
• Hepatopoeitin-A
• Hepatopoietin A
• HGF
• HGFB
• HPTA
• HPTAhepatocyte growth factor
• lung fibroblast-derived mitogen
• Scatter factor
• SF
• SFhepatopoeitin-A

Background

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1-5) Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%-94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up-regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
3. Rosario, M. and Birchmeier, W. (2004) Dev. Cell 7:3.
4. Lesk, A.M. and Fordham, W.D. (1996) J. Mol. Biol. 258:501.
5. Nakamura, T. et al. (1989) Nature 342:440.
6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
10. Montesano, R. et al. (1991) Cell 67:901.
11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
13. 13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
14. 14. Mineo, R. et al. (1994) Endocrinology 145:4355.
15. 15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Publications for HGF (294-HGN)(15)

Publications using 294-HGN

• Cervantes-Villagrana, RD;Mendoza, V;Hinck, CS;de la Fuente-León, RL;Hinck, AP;Reyes-Cruz, G;Vázquez-Prado, J;López-Casillas, F; Betaglycan sustains HGF/Met signaling in lung cancer and endothelial cells promoting cell migration and tumor growth Heliyon 2024-05-15 [PMID: 38756586] (Bioassay, Mouse, Porcine)
• Gallo, S;Vitacolonna, A;Comoglio, PM;Crepaldi, T; MET Oncogene Enhances Pro-Migratory Functions by Counteracting NMDAR2B Cleavage Cells 2023-12-21 [PMID: 38201232] (Cell Culture, Human)
• S Gallo, A Vitacolonn, P Comoglio, T Crepaldi MET Oncogene Controls Invasive Growth by Coupling with NMDA Receptor Cancers, 2022-09-11;14(18):. 2022-09-11 [PMID: 36139568] (Bioassay, Human)
• N Shimamura, K Fujii, S Ohkoshi Detection and quantification of human-specific mRNA from hepatocyte-like cells derived from dental pulp using real-time polymerase chain reaction Journal of oral biosciences, 2021-07-24;0(0):. 2021-07-24 [PMID: 34311038] (Bioassay, Human)
• SR Adame-Garc, RD Cervantes-, LB Orduña-Cas, JC Del Rio, JS Gutkind, G Reyes-Cruz, SS Taylor, J Vázquez-Pr cAMP-dependent activation of the Rac guanine exchange factor P-REX1 by type I protein kinase A (PKA) regulatory subunits J. Biol. Chem., 2018-12-10;0(0):. 2018-12-10 [PMID: 30530493] (Bioassay, Human, Porcine)
• A Jahangiri, A Nguyen, A Chandra, MK Sidorov, G Yagnik, J Rick, SW Han, W Chen, PM Flanigan, D Schneidman, S Mascharak, M De Lay, B Imber, CC Park, K Matsumoto, K Lu, G Bergers, A Sali, WA Weiss, MK Aghi Cross-activating c-Met/?1 integrin complex drives metastasis and invasive resistance in cancer Proc. Natl. Acad. Sci. U.S.A., 2017-09-26;0(0):. 2017-09-26 [PMID: 28973887] (Bioassay, Human)
• BC Narmada, YT Goh, H Li, S Sinha, H Yu, C Cheung Human Stem Cell-Derived Endothelial-Hepatic Platform for Efficacy Testing of Vascular-Protective Metabolites from Nutraceuticals Stem Cells Transl Med, 2016-10-07;6(3):851-863. 2016-10-07 [PMID: 28297582] (Bioassay, Human)
• H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3 Sci Rep, 2017-03-02;7(0):43736. 2017-03-02 [PMID: 28252047] (Bioassay, Human)
• Wang J, Goetsch L, Tucker L, Zhang Q, Gonzalez A, Vaidya K, Oleksijew A, Boghaert E, Song M, Sokolova I, Pestova E, Anderson M, Pappano W, Ansell P, Bhathena A, Naumovski L, Corvaia N, Reilly E Anti-c-Met monoclonal antibody ABT-700 breaks oncogene addiction in tumors with MET amplification. BMC Cancer, 2016-02-16;16(1):105. 2016-02-16 [PMID: 26879245] (Bioassay, Human)
• Tervonen T, Belitskin D, Pant S, Englund J, Marques E, Ala-Hongisto H, Nevalaita L, Sihto H, Heikkila P, Leidenius M, Hewitson K, Ramachandra M, Moilanen A, Joensuu H, Kovanen P, Poso A, Klefstrom J Deregulated hepsin protease activity confers oncogenicity by concomitantly augmenting HGF/MET signalling and disrupting epithelial cohesion. Oncogene, 2015-07-13;0(0):ePub. 2015-07-13 [PMID: 26165838] (Bioassay, Human)
• Basilico C, Pennacchietti S, Vigna E, Chiriaco C, Arena S, Bardelli A, Valdembri D, Serini G, Michieli P Tivantinib (ARQ197) displays cytotoxic activity that is independent of its ability to bind MET. Clin Cancer Res, 2013-03-26;19(9):2381-92. 2013-03-26 [PMID: 23532890] (Bioassay, Human)
• Roelandt P, Pauwelyn KA, Sancho-Bru P Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells. PLoS ONE, 2010-08-11;5(8):e12101. 2010-08-11 [PMID: 20711405] (Cell Culture, Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA (Standard))
• Jin M, Chen Y, He S, Ryan SJ, Hinton DR Hepatocyte growth factor and its role in the pathogenesis of retinal detachment. Invest. Ophthalmol. Vis. Sci., 2004-01-01;45(1):323-9. 2004-01-01 [PMID: 14691191] (In Vivo, Rabbit)
• Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3. Cancer Res., 2003-02-15;63(4):887-92. 2003-02-15 [PMID: 12591743] (Binding Assay, Human)

Bioinformatics

• Gene Symbol: HGF
• Uniprot:
  • P14210.2()