Recombinant Human HGF (NS0 Expressed) Protein, CF

Recombinant Human HGF induces IL-11 secretion by Saos-2 human osteosarcoma cells. The ED50 for this effect is equal to or less than 4.00 ng/mL.

2 μg/lane of Recombinant Human HGF (NSO Expressed) Protein (Catalog # 11352-HG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-40 kDa ( beta chain) & 54-66 kDa ( alpha chain), under reducing conditions.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human HGF (NS0 Expressed) Protein, CF Summary

• Additional Information: Wild type
• Details of Functionality: Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED₅₀ for this effect is equal to or less than 4.00 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human HGF protein
  Gln32-Arg494 alpha chain & Val495-Ser728 beta chain
• Accession #: P14210.2
• N-terminal Sequence: alpha chain: Gln32; deduced from Arg 33 after deblocking
  beta chain; Val495
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.50 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 54 kDa ( alpha chain) & 26 kDa ( beta chain).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30-40 kDa ( beta chain) & 54-66 kDa ( alpha chain), under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute 10 μg size at 100 μg/mL in PBS. Reconstitute all the other sizes at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HGF (NS0 Expressed) Protein, CF

• deafness, autosomal recessive 39
• DFNB39
• EC 3.4.21
• EC 3.4.21.7
• fibroblast-derived tumor cytotoxic factor
• F-TCF
• hepatocyte growth factor (hepapoietin A; scatter factor)
• Hepatopoeitin-A
• Hepatopoietin A
• HGF
• HGFB
• HPTA
• HPTAhepatocyte growth factor
• lung fibroblast-derived mitogen
• Scatter factor
• SF
• SFhepatopoeitin-A

Background

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates human HGF isoforms that lack the proteinase‑like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH‑stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
5. Nakamura, T. et al. (1989) Nature 342:440.
6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
10. Montesano, R. et al. (1991) Cell 67:901.
11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
14. Mineo, R. et al. (1994) Endocrinology 145:4355.
15. Urbanek, K. et al. (2005) Circ. Res. 97:663.

Bioinformatics

• Uniprot:
  • P14210.2()