Recombinant Human HB-EGF Protein, CF


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Applications Bioactivity

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Recombinant Human HB-EGF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 0.15-0.75 ng/mL.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human HB-EGF protein
Accession #
N-terminal Sequence

Asp63 (major), Gly32, Glu24

Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE with silver staining
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW

9.7 kDa

Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
11-20 kDa, reducing conditions
Read Publications using
259-HE/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HB-EGF Protein, CF

  • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor)
  • Dtr
  • DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor)
  • Dts
  • DTSF
  • HB-EGF
  • Hegfl
  • heparin-binding EGF-like growth factor
  • heparin-binding epidermal growth factor
  • proheparin-binding EGF-like growth factor


Human HB-EGF (Heparin-Binding EGF-like growth factor) is a 12-16 kDa member of the EGF family of peptide growth factors (1-3). Also known as the DTR (diphtheria toxin receptor), it is further classified as a group 2 ErbB ligand based on its ability to activate both the EGF/ErbB1 and ErbB4 receptors (4, 5). HB-EGF is synthesized as a 208 amino acid (aa) type I transmembrane preproprecursor (1, 6). It contains a 19 aa signal sequence, a 43 aa prosegment, an 86 aa mature region (aa 63-148), an 11 aa juxtamembrane cleavage peptide, a 24 aa transmembrane segment, and a 25 aa cytoplasmic tail (aa 184-208). As an integral membrane protein, HB-EGF is expressed as a 19-27 kDa protein in mammalian cells (7-9). The variability in molecular weight (MW) is attributed to heterogeneity in glycosylation and/or the utilization of multiple proteolytic cleavage sites during maturation. Mature HB-EGF is a soluble peptide that arises from proteolytic processing of the transmembrane form. It possesses an EGF-like domain between aa 104-144, and a heparin-binding motif between aa 93‑113. Although the aa range for "mature" HB-EGF is typically stated to be Asp63-Leu148, potential N-terminal start (cleavage) sites also exist at Gly32, Arg73, Val74, Ser77 and Ala82 (8, 10-12). Thus, differential processing (in part) likely accounts for the 16-23 kDa range in MW noted for mammalian-derived mature HB-EGF. Proteases suggested to contribute to HB-EGF processing include TACE, MMP-3 and -7, ADAM-17 and ADAM-12 (11, 13-16). When expressed recombinantly in E.coli, HB-EGF (aa 73-148) runs at 14 kDa in SDS-PAGE; when expressed in Baculovirus, HB-EGF (aa 63-148, 77-148 and 32-148) runs at 18 kDa, 15 kDa, and 19 kDa respectively (8, 12, 17). Over aa 63-148, human HB-EGF- shares 76% and 73% aa sequence identity with rat and mouse HB-EGF, respectively (1, 18). Cells known to express HB-EGF include bronchial epithelium (19), visceral and vascular smooth muscle (20, 21), CD4+ T cells (22), cardiac muscle (23), glomerular podocytes (24), keratinocytes (13) and IL-10-secreting regulatory macrophages (25). As noted earlier, HB-EGF is known to bind to both 170 kDa EGFR and 180 kDa ErbB4, and through heterodimerization, ErbB2 (13, 26). Activity associated with ErbB4 binding appears to be limited to non-mitogenic actions, while EGFR binding induces both mitogenic and non-mitogenic activity.
  1. Higashiyama, S et al. (1991) Science 251:936.
  2. Schneider, MR & E. Wolf (2009) J. Cell. Physiol. 218:460.
  3. G.V. Sherbet (2011) The Epidermal Growth Factor (EGF) Family in Growth Factors and Their Receptors in Cell Differentiation, Cancer and Cancer Therapy, Pages 173-198. Elsevier, NY.
  4. Iwamoto, R & E. Mekada (2000) Cytokine Growth Factor Rev. 11:335.
  5. Miyata, K. et al. (2012) Anticancer Res. 32:2347.
  6. SwissProt:Q99075.
  7. Raab, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:592.
  8. Nakagawa, T. et al. (1996) J. Biol. Chem. 271:30858.
  9. Higashiyama, S. et al. (1995) J. Cell Biol. 128:929.
  10. Higashiyama, S. et al. (1992) J. Biol. Chem. 267:6205.
  11. Hinkle, C.L. et al. (2004) J. Biol. Chem. 279:24179.
  12. Ono, M. et al. (1994) J. Biol. Chem. 269:31315.
  13. Nanba, D. & S. Higashiyama (2004) Cytokine Growth Factor Rev. 15:13.
  14. Cheng, K. et al. (2007) Biochem. Pharmacol. 73:1001.
  15. Suzuki, M. et al. (1997) J. Biol. Chem. 272:31730.
  16. Asakura, M. et al. (2002) Nat. Med. 8:35.
  17. Marikovsky, M. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3889.
  18. Abraham, J.A. et al. (1993) Biochem. Biophys. Res. Commun. 190:125.
  19. Tschumperlin, D.J. et al. (2004) Nature 429:83.
  20. Park, J.M. et al. (1998) Am. J. Physiol. 275:C1247.
  21. Miyagawa, J. et al. (1995) J. Clin. Invest. 95:404.
  22. Blotnick, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:2890.
  23. Iwamoto, R. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3221.
  24. Bollee, G. et al. (2011) Nat. Med. 17:1242.
  25. Edwards, J.P. et al. (2009) J. Immunol. 182:1929.
  26. Elenius, K. et al. (1997) EMBO J. 16:1268.

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Publications for HB-EGF (259-HE/CF)(57)

We have publications tested in 5 confirmed species: Human, Mouse, Fish - Prionace glauca (Blue Shark), N/A, Zebrafish.

We have publications tested in 7 applications: Bioassay, Cell Culture, ELISA (Standard), ELISA Development, ELISA Standard, In Vivo, In vivo.

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Fish - Prionace glauca (Blue Shark)
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Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using 259-HE/CF Applications Species
M Shibuya, H Matsui, T Sasagawa, T Nagamatsu A simple detection method for the serum sFLT1 protein in preeclampsia Scientific Reports, 2021;11(1):20613. 2021 [PMID: 34663835] (ELISA Standard, Human) ELISA Standard Human
G Parkinson, P Roboti, L Zhang, S Taylor, P Woodman His domain protein tyrosine phosphatase and Rabaptin-5 couple endo-lysosomal sorting of EGFR with endosomal maturation Journal of Cell Science, 2021;134(21):. 2021 [PMID: 34657963] (Bioassay, Human) Bioassay Human
HF Yu, LW Zheng, ZQ Yang, YS Wang, TT Wang, ZP Yue, B Guo TAZ as a novel regulator of oxidative damage in decidualization via Nrf2/ARE/Foxo1 pathway Experimental & Molecular Medicine, 2021;0(0):. 2021 [PMID: 34497345] (Bioassay, Mouse) Bioassay Mouse
B Ehst, Z Wang, J Leitenberg, D McClanahan, R De La Torr, E Sawka, AG Ortega-Loa, J Strunck, T Greiling, E Simpson, Y Liu Synergistic induction of IL-23 by TNF&amp;alpha, IL-17A, and EGF in keratinocytes Cytokine, 2020;0(0):155357. 2020 [PMID: 33153894] (Cell Culture, Human) Cell Culture Human
KC Allan, LR Hu, MA Scavuzzo, AR Morton, AS Gevorgyan, EF Cohn, BLL Clayton, IR Bederman, S Hung, CF Bartels, M Madhavan, PJ Tesar Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33091368] (Bioassay, Mouse) Bioassay Mouse
EJ Kim, YJ Kim, HI Lee, SH Jeong, HJ Nam, JH Cho NRF2 Knockdown Resensitizes 5-Fluorouracil-Resistant Pancreatic Cancer Cells by Suppressing HO-1 and ABCG2 Expression Int J Mol Sci, 2020;21(13):. 2020 [PMID: 32629871] (Cell Culture, Human) Cell Culture Human
E Fu, T Liu, S Yu, X Chen, L Song, H Lou, F Ma, S Zhang, S Hussain, J Guo, J Sun, P Yu, X Mao, L Wei M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB?EGF Oncol. Rep., 2020;44(2):698-710. 2020 [PMID: 32627036] (Cell Culture, Human) Cell Culture Human
HF Yu, CC Duan, ZQ Yang, YS Wang, ZP Yue, B Guo HB-EGF Ameliorates Oxidative Stress-Mediated Uterine Decidualization Damage Oxid Med Cell Longev, 2019;2019(0):6170936. 2019 [PMID: 31885807] (Cell Culture, Mouse) Cell Culture Mouse
L Messerschm, S Fischer, P Wiedemann, A Bringmann, M Hollborn Osmotic induction of cyclooxygenase-2 in RPE cells: Stimulation of inflammasome activation Mol. Vis., 2019;25(0):329-344. 2019 [PMID: 31341381] (Bioassay, Human) Bioassay Human
A Hamon, D García-Gar, D Ail, J Bitard, A Chesneau, D Dalkara, M Locker, JE Roger, M Perron Linking YAP to M�ller Glia Quiescence Exit in the Degenerative Retina Cell Rep, 2019;27(6):1712-1725.e6. 2019 [PMID: 31067458] (In Vivo, Mouse) In Vivo Mouse
Show All 57 Publications.

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Gene Symbol HBEGF