Recombinant Human Betacellulin Protein


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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Betacellulin Protein Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 0.15-0.6 ng/mL.
E. coli-derived human Betacellulin/BTC protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
15 kDa, reducing conditions
Read Publications using
261-CE in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Betacellulin Protein

  • Betacellulin
  • BTC
  • probetacellulin


Betacellulin (BTC) is a new member of the EGF family of cytokines that is comprised of at least ten proteins including EGF, TGF-alpha, amphiregulin, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or more EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein composed of 80 amino acid residues. The cDNA encoding human BTC was cloned from a human breast adenocarcinoma cell line (MCF-7) cDNA library. Human and mouse cDNAs encode BTC precursor proteins of 178 and 177 amino acid residues, respectively. At the amino acid sequence level, human BTC precursor protein exhibits 79% identity with that of the mouse BTC precursor. In a mouse cell line transfected with human BTC cDNA, three forms of soluble human BTC have been detected: the glycosylated, intact BTC composed of 80 amino acid residues, a truncated molecule lacking 12 amino acid residues from the amino terminus, and a second truncated molecule lacking 30 amino acid residues from the amino terminus. The biological activities of these BTC forms were shown to be identical. BTC can bind to the EGF receptor and is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells.

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Publications for Betacellulin/BTC (261-CE)(9)

We have publications tested in 4 confirmed species: Human, Hamster, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 4 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard).

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Cell Culture
ELISA (Standard)
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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
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Showing Publications 1 - 9 of 9.
Publications using 261-CE Applications Species
NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020;0(0):. 2020 [PMID: 32094658] (Cell Culture, Human) Cell Culture Human
Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020;21(3):274-286. 2020 [PMID: 32066947] (Cell Culture, Human) Cell Culture Human
J Zhao, C Klausen, Y Yi, JC Cheng, HM Chang, PCK Leung Betacellulin enhances ovarian cancer cell migration by up-regulating Connexin43 via MEK-ERK signaling Cell. Signal., 2019;65(0):109439. 2019 [PMID: 31654720] (Bioassay, Human) Bioassay Human
C Zou, Y Lu, X Teng, S Wang, X Sun, F Huang, G Shu, X Huang, H Guo, Z Chen, J Zhang, YA Zhang MRI tracking of autologous pancreatic progenitor-derived insulin-producing cells in monkeys Sci Rep, 2017;7(1):2505. 2017 [PMID: 28566744] (Bioassay, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) Bioassay Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Tom J Parry Effects of neuregulin GGF2 (cimaglermin alfa) dose and treatment frequency on left ventricular function in rats following myocardial infarction Eur. J. Pharmacol, 2016;796(0):76-89. 2016 [PMID: 27993643] (Bioassay, Human) Bioassay Human
Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke. Neuropharmacology, 2010;59(7):640-9. 2010 [PMID: 20691195] (Binding Assay, Human) Binding Assay Human
Shin S, Li N, Kobayashi N, Yoon JW, Jun HS Remission of diabetes by beta-cell regeneration in diabetic mice treated with a recombinant adenovirus expressing betacellulin. Mol. Ther., 2008;16(5):854-61. 2008 [PMID: 18388935] (ELISA (Standard), N/A) ELISA (Standard) N/A
Petropavlovskaia M, Makhlin J, Sampalis J, Rosenberg L Development of an in vitro pancreatic tissue model to study regulation of islet neogenesis associated protein expression. J. Endocrinol., 2006;191(1):65-81. 2006 [PMID: 17065390] (Bioassay, Hamster) Bioassay Hamster
Sanderson MP, Abbott CA, Tada H, Seno M, Dempsey PJ, Dunbar AJ Hydrogen peroxide and endothelin-1 are novel activators of betacellulin ectodomain shedding. J. Cell. Biochem., 2006;99(2):609-23. 2006 [PMID: 16676357] (ELISA (Standard), N/A) ELISA (Standard) N/A

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Gene Symbol BTC