HB-EGF: Novus Biologicals

AF-259-NA

HB-EGF Antibody [Unconjugated]

250 μg

AF-259-NA

259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB-EGF (20 ng/mL) is neutralized (green line) by increasing concentrations of Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA). The ND₅₀ is typically 0.1-0.6 µg/mL." class="big_lightbox">

Recombinant Human HB-EGF (Catalog # <a class=

259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB-EGF (20 ng/mL) is neutralized (green line) by increasing concentrations of Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA). The ND₅₀ is typically 0.1-0.6 µg/mL." alt="Recombinant Human HB-EGF (Catalog # 259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB-EGF (20 ng/mL) is neutralized (green line) by increasing concentrations of Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA). The ND₅₀ is typically 0.1-0.6 µg/mL." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑259‑NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

HB-EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Human HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Dynamic expression and processing of Tgf-alpha and Hb-egf during multistep pancreatic neuroendocrine tumorigenesis (PNET). (A) Relative expression of Egf family members in cDNAs prepared from total RNA extracts of successive stages of neoplastic progression (NI = normal islets; HI = hyperplastic islets; AI = angiogenic islets; IT = islet tumors). Levels of mRNAs are expressed as a percentage of the mGus control mRNA. (B) Western blot analysis of the various forms of Tgf-alpha polypeptides in total protein extracts from successive stages of disease progression. (C) Analogous Western blot analysis of the various forms of Hb-egf polypeptides in total protein extracts from the successive disease stages. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20975924), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>BHLHE40-knockdown reduced hypoxia-induced expression of a panel of cytokines and growth factors. a Heatmaps of cytokines and growth factors whose hypoxia-induced expression (1% O2 at 6 h or 48 h, fold-change ≥ 1.5 in two independent experiments) was diminished by BHLHE40-knockdown (KD) in LM cells. The gene expression levels were determined using the Illumina Human HT-12 expression BeadChips. Normalized (quantile normalization) hybridization signals were log2 transformed and standardized by genes across experiment conditions to generate the heatmap. b Heatmaps of a subset of genes list in a whose expression was affected by BHLHE40-KD in LM cells exposed to hypoxia combined with low (1 mM) glucose (1%O2/LG, 4 h). The gene expression levels were determined using the Affymetrix Human Gene 1.0 ST array. c Heatmaps of hypoxia-induced genes whose expression was not significantly affected by BHLHE40-KD in LM cells as determined by the Illumina Human HT-12 expression BeadChips. d Expression of luciferase reporters driven by hypoxia-responsive elements of ITGA6 or LDHA was not affected by BHLHE40 knockout (KO) by CRISPR/Cas9 editing in MDA-MB-231 cells, in the absence or presence of exogenous HIF1A. Luciferase activities were normalized to co-transfected CMV-beta -galactosidase and presented as mean ± SD (n = 6). e Expression of genes in control LM empty vector (EV) and LM BHLHE40-KD cells exposed to 1%O2/LG (4 h). mRNA expression levels were determined by qPCR, normalized to RPL13A, and presented as mean ± SD (n = 6). *p < 0.05 (n = 6, 1%O2/LG vs. untreated control), **p < 0.05 (n = 6, KD vs. EV), one-way ANOVA followed by Tukey’s post-hoc tests. f mRNA and protein expression levels of HBEGF and CTGF in primary xenograft tumors, determined by qPCR and immunoblotting, respectively. *p < 0.05 (n = 6, KD vs. EV), Student’s t test. Representative immunoblotting images of three tumors of KD or EV cells are presented Image collected and cropped by CiteAb from the following publica

<b>Biological Strategies Validation. </b>Egfr activity contributes to RT2 tumor growth and angiogenic switching. (A) Relative expression quantified by real-time quantitative PCR of Erbb family members in cDNAs derived from total RNA extracts of successive stages of pancreatic neuroendocrine tumorigenesis from RIP1-Tag2 (RT2) transgenic mice (NI = normal islets; HI = hyperplastic islets; AI = angiogenic islets; IT = islet tumors). Levels of mRNAs are expressed as a percentage of the mGus control mRNA. (B) Western blot analysis of protein extracts from successive stages of RT2-derived lesions. (C) Western blot analysis of protein extracts from RT2-derived tumors 4 h after treatment with a vehicle solution or erlotinib (80 mg/kg). (D-F) Comparison of the average tumor burden of RT2 mice treated daily with a control solution (vehicle) or with (D) gefitinib (80 mg/kg), (E) CI-1033 (80 mg/kg), both from 11.5 to 14.5 weeks of age, or with (F) erlotinib (80 mg/kg), from 12 to 16 weeks of age with an additional vehicle-treated time point at 14 weeks. (G) Comparison of the average number of hemorrhagic angiogenic islets per pancreas of RT2 mice treated daily with a vehicle solution or with erlotinib (80 mg/kg) or CI-1033 (80 mg/kg) from 6 to 9 weeks of age. (N = number of animals per treatment group). *P < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20975924), licensed under a CC-BY license. Not internally tested by R&D Systems.

Hb-egf contributes to the angiogenic switch and neovascularization of PNET tumors. (A) Comparison of the average number of hemorrhagic angiogenic islets in wild-type (wt) and Hb-egf mutant (Hb) 9-week-old RT2 mice. (B-C) Comparison of the average (B) tumor burden or (C) tumor number in wild-type and Hb-egf mutant 14-week-old RT2 mice. (D) Western blot analysis of Hb-egf protein expression in total protein extracts from pools of 5 wild-type or 5 Hb-egf mutant tumors. (E-F) Average percentage of (E) dividing tumor cells (phospho-histone H3 positive) or (F) apoptotic cells (TdT-mediated dUTP-biotin nick end-labeling [TUNEL] positive) in wild-type or Hb-egf mutant tumors at 14 weeks of age. (G) Average vessel density (ratio of Meca-32 stained area to total section area) in wild-type or Hb-egf mutant tumors at 14 weeks of age. (H-I) Representative micrographs of (H) wild-type or (I) Hb-egf mutant RT2 tumors stained with Meca-32 (red) and DAPI (blue) (200x); panels are representative of 20 wild-type and 18 Hb-egf RT2 tumors dissected from at least 5 independent mice of each genotype. (N = number of independent mice [A-C] or tumor fields [E-G] analyzed per genotype; 1-2 fields per tumor.) *P < 0.001. **P < 0.01. ***P < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20975924), licensed under a CC-BY license. Not internally tested by R&D Systems.

259-HE) was serially diluted and captured by Mouse Anti-Human HB‑EGF Monoclonal Antibody (Catalog # MAB2592) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human HB‑EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

259-HE) was serially diluted and captured by Mouse Anti-Human HB‑EGF Monoclonal Antibody (Catalog # MAB2592) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human HB‑EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

259-HE) was serially diluted and captured by Mouse Anti-Human HB‑EGF Monoclonal Antibody (Catalog # MAB2592) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human HB‑EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

259-HE) was serially diluted and captured by Mouse Anti-Human HB‑EGF Monoclonal Antibody (Catalog # MAB2592) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human HB‑EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-259-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-259-NAU-100UG
Alexa Fluor 405	AF-259-NAV-100UG
Alexa Fluor 488	AF-259-NAG-100UG
Alexa Fluor 532	AF-259-NAX-100UG
Alexa Fluor 594	AF-259-NAT-100UG
Alexa Fluor 647	AF-259-NAR-100UG
Alexa Fluor 700	AF-259-NAN-100UG
Alexa Fluor 750	AF-259-NAS-100UG
Alexa Fluor Plus 405	AF-259-NAAFP405
Alexa Fluor Plus 488	AF-259-NAAFP488
Alexa Fluor Plus 555	AF-259-NAAFP555
Alexa Fluor Plus 594	AF-259-NAAFP594
Alexa Fluor Plus 647	AF-259-NAAFP647
Alexa Fluor Plus 680	AF-259-NAAFP680
Allophycocyanin	AF-259-NAA
Biotin	BAF259
CoraFluor 1	AF-259-NACL1
DyLight 350	AF-259-NAD
DyLight 405	AF-259-NAE
DyLight 488	AF-259-NAK
DyLight 550	AF-259-NAL
DyLight 594	AF-259-NAM
DyLight 650	AF-259-NAW
DyLight 680	AF-259-NAY
DyLight 755	AF-259-NAZ
FITC	AF-259-NAF
HRP	AF-259-NAH
Janelia Fluor 525	AF-259-NAJF525
Janelia Fluor 549	AF-259-NAI
Janelia Fluor 646	AF-259-NAJ
Janelia Fluor 669	AF-259-NAJF669
PE	AF-259-NAP
PerCP	AF-259-NAC
mFluor Violet 450 SE	AF-259-NAMFV450
mFluor Violet 500 SE	AF-259-NAMFV500
mFluor Violet 610 SE	AF-259-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-259-SP

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259-HE

Recombinant Human HB-EGF Protein

(71 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	259-HE-050/CF
Carrier Free	259-HE-250/CF

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AF8239

HB-EGF Antibody [Unconjugated]

(10 Publications)

100 μg

AF8239

HAF016). A specific band was detected for HB-EGF at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8239) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for HB-EGF at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8239) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HB-EGF at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

HB-EGF was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse HB-EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8239) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing central nervous system. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF8239U-100UG
Alexa Fluor 405	AF8239V-100UG
Alexa Fluor 488	AF8239G-100UG
Alexa Fluor 532	AF8239X-100UG
Alexa Fluor 594	AF8239T-100UG
Alexa Fluor 647	AF8239R-100UG
Alexa Fluor 700	AF8239N-100UG
Alexa Fluor 750	AF8239S-100UG
Alexa Fluor Plus 405	AF8239AFP405-100UG
Alexa Fluor Plus 488	AF8239AFP488-100UG
Alexa Fluor Plus 555	AF8239AFP555-100UG
Alexa Fluor Plus 594	AF8239AFP594-100UG
Alexa Fluor Plus 647	AF8239AFP647-100UG
Alexa Fluor Plus 680	AF8239AFP680-100UG
Allophycocyanin	AF8239A
Biotin	AF8239B
CoraFluor 1	AF8239CL1
DyLight 350	AF8239D
DyLight 405	AF8239E
DyLight 488	AF8239K
DyLight 550	AF8239L
DyLight 594	AF8239M
DyLight 650	AF8239W
DyLight 680	AF8239Y
DyLight 755	AF8239Z
FITC	AF8239F
HRP	AF8239H
Janelia Fluor 525	AF8239JF525
Janelia Fluor 549	AF8239I
Janelia Fluor 646	AF8239J
Janelia Fluor 669	AF8239JF669
PE	AF8239P
PerCP	AF8239C
mFluor Violet 450 SE	AF8239MFV450
mFluor Violet 500 SE	AF8239MFV500
mFluor Violet 610 SE	AF8239MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF8239-SP

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NBP2-35069

Recombinant Mouse HB-EGF Protein

(2 Publications)

10μg

NBP2-35069

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Sizes:

Sample Size Available

Sizes: Sample Size Available

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier-Free	NBP2-35069-1mg
Carrier-Free	NBP2-35069-100ug
Carrier-Free	NBP2-35069-500ug

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MAB2591

HB-EGF Antibody (406316)

(3 Publications)

100 μg

MAB2591

259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB‑EGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Human HB-EGF Monoclonal Antibody (Catalog # MAB2591). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_lightbox">

259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB‑EGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Human HB-EGF Monoclonal Antibody (Catalog # MAB2591). The ND₅₀ is typically 0.5-2.5 µg/mL." alt="Recombinant Human HB‑EGF (Catalog # 259-HE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HB‑EGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Human HB-EGF Monoclonal Antibody (Catalog # MAB2591). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_thumb" />

Signaling pathways activated by PDGF-AA, PDGF-BB, HB-EGF, TCM, or VECM.(A) Decidualized St-T1b were starved in Opti-MEM and then treated for 5 or 30 min with PDGF-BB, HB-EGF, TCM or PDGF-AA. Levels of phosphorylated (p-) or total ERK1/2, Akt, and p38 were determined by Western blotting. (B) Decidualized hESC were starved in Opti-MEM and then treated for 5 min with PDGF-BB, HB-EGF, TCM, 5 individual VECM preparations, villous explant control medium (VECM-Co), or PDGF-AA. Western blotting was performed as above. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23349855), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression and regulation of HER1 in St-T1b cells and hESCs.(A) Effect of decidualization on HER1 expression. Whole cell extracts from two individual hESC preparations or from St-T1b cells, non-decidualized (−) or decidualized (+), were immunoblotted for HER1 (antibody from New England Biolabs), and for GAPDH as a loading control. In addition, the levels of Nur77, alpha -SMA, MLC2 and phospho-MLC2 were analyzed. (B, C, D) Time-dependent downregulation of HER1 in response to HB-EGF or TCM. Non-decidualized (ND) or decidualized (D) St-T1b cells, maintained in Opti-MEM, were incubated with PDGF-BB, HB-EGF or TCM (20 or 100%) for 5 min (B) or 5, 30 and 60 min (C) and subjected to Western Blot analysis for HER1 (antibody from Santa Cruz). Decidualized St-T1b cells were stimulated with HB-EGF or TCM (100%) for 5, 30 and 60 min or 24 h (D) and analyzed for HER1 by Western blotting (antibody from Santa Cruz), followed by immunoblotting for phospho-ERK1/2 and phosphorylated (Ser473) and total Akt. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23349855), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2591U-100UG
Alexa Fluor 405	FAB2591V-100UG
Alexa Fluor 488	FAB2591G-100UG
Alexa Fluor 532	FAB2591X-100UG
Alexa Fluor 594	FAB2591T-100UG
Alexa Fluor 647	FAB2591R-100UG
Alexa Fluor 700	FAB2591N-100UG
Alexa Fluor 750	FAB2591S-100UG
Alexa Fluor Plus 405	FAB2591AFP405-100UG
Alexa Fluor Plus 488	FAB2591AFP488-100UG
Alexa Fluor Plus 555	FAB2591AFP555-100UG
Alexa Fluor Plus 594	FAB2591AFP594-100UG
Alexa Fluor Plus 647	FAB2591AFP647-100UG
Alexa Fluor Plus 680	FAB2591AFP680-100UG
Allophycocyanin	FAB2591A
Biotin	FAB2591B
CoraFluor 1	FAB2591CL1
DyLight 350	FAB2591D
DyLight 405	FAB2591E
DyLight 488	FAB2591K
DyLight 550	FAB2591L
DyLight 594	FAB2591M
DyLight 650	FAB2591W
DyLight 680	FAB2591Y
DyLight 755	FAB2591Z
FITC	FAB2591F
HRP	FAB2591H
Janelia Fluor 525	FAB2591JF525
Janelia Fluor 549	FAB2591I
Janelia Fluor 646	FAB2591J
Janelia Fluor 669	FAB2591JF669
PE	FAB2591P
PerCP	FAB2591C
mFluor Violet 450 SE	FAB2591MFV450
mFluor Violet 500 SE	FAB2591MFV500
mFluor Violet 610 SE	FAB2591MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2591-SP
Carrier Free	MAB2591-500

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DHBEG0

Human HB-EGF Quantikine ELISA Kit

(5 Publications)

1 Kit

DHBEG0

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB259

HB-EGF Antibody (125923) [Unconjugated]

(2 Publications)

100 μg

MAB259

CXCL12 modifies HB-EGF expression in mononuclear phagocytes. Human mononuclear phagocytes (Mø) were cultured alone or in the presence of 200 ng/mL CXCL12. Cells were collected after 20 minutes, 2 hours and 24 hours; cell-free supernatants were collected after 24 hours and the levels of soluble HB-EGF protein were measured using a specific ELISA. (A) Flow cytometric analysis showing that CXCL12-stimulated Mø released HB-EGF (after 20 minutes) and up-regulated its expression (after 24 hours). (B) Northern blot analysis on Mø and neutrophils (PMN, used as negative control) collected after 2 hours of stimulation with CXCL12. Only Mø produced detectable levels of HB-EGF mRNA in basal conditions, and HB-EGF transcripts were up-regulated upon stimulation. After 24 hours, the mRNA up-regulation persisted (data not shown). (C) CXCL12 treatment induced Mø to release HB-EGF into the culture medium (p < 0.05). Representative pictures or the means ± SD out of 10 experiments are shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20946648), licensed under a CC-BY license. Not internally tested by R&D Systems.

Knockdown of GM-CSF protein levels after siRNA application in cancer cells. HeLa/DLD-1 cells were transfected with control siRNA (1/1*, 2/2*) or GM-CSF siRNA (3/3*, 4/4*) and cultured in the absence or presence of 25 ng/mL HB-EGF. The numbers indicate the culture conditions and the corresponding supernatants (SN) used for ELISA or cell stimulation. (A) Blockade of GM-CSF production in cultures of HeLa/DLD-1 cells transfected with GM-CSF siRNA was confirmed by immunocytochemistry (2/2* vs. 4/4*) and ELISA (left side; 2/2* vs. 4/4*, p < 0.05). (B) SN from GM-CSF-silenced HeLa/DLD-1 did not induce HB-EGF expression in mononuclear phagocytes (Mø), as revealed by flow cytometry (2/2* vs. 4/4*) and ELISA (left side; 2/2* vs. 4/4*, p < 0.05). (C) Mø stimulated with SN from GM-CSF-silenced HeLa/DLD-1 cells released SN less effective at inducing GM-CSF in non-silenced cancer cells, as determined by ELISA (see Methods section; SN2 vs. SN4, p < 0.05). Representative pictures or the means ± SD out of 5 experiments are shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20946648), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB259U
Alexa Fluor 405	FAB259V
Alexa Fluor 488	IC259G
Alexa Fluor 532	FAB259X
Alexa Fluor 594	FAB259T
Alexa Fluor 647	FAB259R
Alexa Fluor 700	FAB259N
Alexa Fluor 750	FAB259S
Allophycocyanin	IC259A
Biotin	FAB259B
CoraFluor 1	FAB259CL1
DyLight 350	FAB259D
DyLight 405	FAB259E
DyLight 488	FAB259K
DyLight 550	FAB259L
DyLight 594	FAB259M
DyLight 650	FAB259W
DyLight 680	FAB259Y
DyLight 755	FAB259Z
FITC	FAB259F
HRP	FAB259H
Janelia Fluor 525	FAB259JF525
Janelia Fluor 549	FAB259I
Janelia Fluor 646	FAB259J
Janelia Fluor 669	FAB259JF669
PE	FAB259P
PerCP	FAB259C
mFluor Violet 450 SE	FAB259MFV450
mFluor Violet 500 SE	FAB259MFV500
mFluor Violet 610 SE	FAB259MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC259A
Carrier Free	IC259G
Carrier Free	MAB259-SP
Carrier Free	MAB259-500

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NBP2-62780

Mouse HB-EGF ELISA Kit (Chemiluminescence)

(1 Publication)

1 Kit

NBP2-62780

Mouse HB-EGF ELISA Kit (Chemiluminescence) - Stadnard Curve Reference

ELISA: Mouse HB-EGF ELISA Kit (Chemiluminescence) [NBP2-62780] - Standard Curve Reference

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

7.81-500 pg/mL

Sensitivity:

4.69 pg/mL

Sample Type:

Serum, plasma and other biological fluids

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NBP2-14082

HB-EGF Antibody - BSA Free

0.1 ml

NBP2-14082

Immunohistochemistry-Paraffin: HB-EGF Antibody [NBP2-14082] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: HB-EGF Antibody [NBP2-14082] - Staining of human liver shows no positivity in hepatocytes as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-14082-25ul

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NBP3-32418

HB-EGF Antibody (JE34-79)

100 ul

NBP3-32418

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-15422

HB-EGF Antibody (9C2M6)

100 ul

NBP3-15422

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-34920

Recombinant Human HB-EGF Protein

(1 Publication)

10μg

NBP2-34920

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Sizes:

Sample Size Available

Sizes: Sample Size Available

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier-Free	NBP2-34920-1mg
Carrier-Free	NBP2-34920-100ug
Carrier-Free	NBP2-34920-500ug

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Kit Type

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

HBEGF Products By Gene Name

HB-EGF Antibody [Unconjugated]

Recombinant Human HB-EGF Protein

HB-EGF Antibody [Unconjugated]

Recombinant Mouse HB-EGF Protein

HB-EGF Antibody (406316)

Human HB-EGF Quantikine ELISA Kit

HB-EGF Antibody (125923) [Unconjugated]

Mouse HB-EGF ELISA Kit (Chemiluminescence)

HB-EGF Antibody - BSA Free

HB-EGF Antibody (JE34-79)

HB-EGF Antibody (9C2M6)

Recombinant Human HB-EGF Protein