Recombinant Human GM-CSF Protein

Images

 
1 μg/lane of Recombinant Human GM-CSF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human GM-CSF (Catalog # 215-IL) stimulates cell proliferation of theTF-1 human erythroleukemic cell line. The ED50 for this effect is 6-30 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA

Order Details

Recombinant Human GM-CSF Protein Summary

Additional Information
A New and Improved rhGM-CSF is Now Available! It has a lower endotoxin spec and is CHO expressed!
New Product 7954-GM
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 6-30 pg/mL.
The specific activity of Recombinant Human GM-CSF is approximately 1.5 x 104 IU/μg, which is calibrated against human GM-CSF WHO International Standard (NIBSC code: 88/646).
Source
E. coli-derived
Ala18-Glu144
Accession #
N-terminal Sequence
Ala18
Protein/Peptide Type
Recombinant Proteins
Gene
CSF2
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
215-GM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GM-CSF Protein

  • colony stimulating factor 2 (granulocyte-macrophage)
  • Colony-stimulating factor
  • CSF
  • CSF2
  • GMCSF
  • GM-CSF
  • GMCSFgranulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • MGC131935
  • MGC138897
  • molgramostin
  • sargramostim

Background

GM‑CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte‑macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM‑CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM‑CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM‑CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy‑induced neutropenia, and GM‑CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM‑CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled
alpha -helices (8‑12). Mature human GM‑CSF shares 63%‑70% amino acid sequence identity with canine, feline, porcine, and rat GM‑CSF and 54% with mouse GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta  chain (CD131) which is also a component of the high‑affinity receptors for IL‑3 and IL‑5 (13, 14). In addition, GM‑CSF binds a naturally occurring soluble form of GM‑CSF R alpha (15). Human GM‑CSF is active on canine and feline cells but not on murine cells (16‑18).

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Publications for (183)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 5 applications: Bioassay, Cell Culture Substrate, Ctrl, In Vivo, cell culture.


Filter By Application
Bioassay
(175)
Cell Culture Substrate
(2)
Ctrl
(1)
In Vivo
(2)
cell culture
(3)
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Human
(173)
Mouse
(2)
Rat
(2)
Canine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(3)
Rabbit
(1)
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Showing Publications 1 - 10 of 183. Show All 183 Publications.
Publications using 215-GM Applications Species
H Nishi, K Furuhashi, X Cullere, G Saggu, MJ Miller, Y Chen, F Rosetti, SL Hamilton, L Yang, SP Pittman, J Liao, JM Herter, JC Berry, DJ DeAngelo, C Zhu, GC Tsokos, TN Mayadas Neutrophil Fc?RIIA promotes IgG-mediated glomerular neutrophil capture via Abl/Src kinases J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28891817] (Bioassay, Human) Bioassay Human
L Butcher, M Ahluwalia, T Örd, J Johnston, RH Morris, E Kiss-Toth, T Örd, JD Erusalimsk Evidence for a role of TRIB3 in the regulation of megakaryocytopoiesis Sci Rep, 2017;7(1):6684. 2017 [PMID: 28751721] (Bioassay, Human) Bioassay Human
J Helft, F Anjos-Afon, AG van der Ve, P Chakravart, D Bonnet, C Reis E Sou Dendritic Cell Lineage Potential in Human Early Hematopoietic Progenitors Cell Rep, 2017;20(3):529-537. 2017 [PMID: 28723558] (Bioassay, Human) Bioassay Human
JH von Steman, AS Rigas, LW Thørner, DGK Rasmussen, OB Pedersen, K Rostgaard, C Erikstrup, H Ullum, MB Hansen Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study PLoS ONE, 2017;12(6):e0179981. 2017 [PMID: 28665954] (Ctrl, Human) Ctrl Human
JT Schroeder, AP Bieneman Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen J. Immunol., 2017;0(0):. 2017 [PMID: 28652400] (Bioassay, Human) Bioassay Human
S Scholz, F Baharom, G Rankin, KT Maleki, S Gupta, S Vangeti, J Pourazar, A Discacciat, J Höijer, M Bottai, NK Björkström, J Rasmuson, M Evander, A Blomberg, HG Ljunggren, J Klingström, C Ahlm, A Smed-Sören Human hantavirus infection elicits pronounced redistribution of mononuclear phagocytes in peripheral blood and airways PLoS Pathog., 2017;13(6):e1006462. 2017 [PMID: 28640917] (Bioassay, Human) Bioassay Human
AA Panackal, LB Rosen, G Uzel, MJ Davis, G Hu, A Adeyemo, F Tekola-Aye, A Lisco, C Diachok, JD Kim, D Shaw, I Sereti, J Stoddard, J Niemela, SD Rosenzweig, JE Bennett, PR Williamson Susceptibility to Cryptococcal Meningoencephalitis Associated With Idiopathic CD4(+) Lymphopenia and Secondary Germline or Acquired Defects Open Forum Infect Dis, 2017;4(2):ofx082. 2017 [PMID: 28638843] (Bioassay, Human) Bioassay Human
AM Clemente, G Castronovo, A Antonelli, MM D'Andrea, M Tanturli, E Perissi, S Paccosi, A Parenti, F Cozzolino, GM Rossolini, MG Torcia Differential Th17 response induced by the two clades of the pandemic ST258 Klebsiella pneumoniae clonal lineages producing KPC-type carbapenemase PLoS ONE, 2017;12(6):e0178847. 2017 [PMID: 28586386] (Bioassay, Human) Bioassay Human
CSB Viegas, RM Costa, L Santos, PA Videira, Z Silva, N Araújo, AL Macedo, AP Matos, C Vermeer, DC Simes Gla-rich protein function as an anti-inflammatory agent in monocytes/macrophages: Implications for calcification-related chronic inflammatory diseases PLoS ONE, 2017;12(5):e0177829. 2017 [PMID: 28542410] (Bioassay, Human) Bioassay Human
J Wang, H Ye, D Zhang, K Cheng, Y Hu, X Yu, L Lu, J Hu, C Zuo, B Qian, Y Yu, S Liu, G Liu, C Mao, S Liu Cancer-derived Circulating MicroRNAs Promote Tumor Angiogenesis by Entering Dendritic Cells to Degrade Highly Complementary MicroRNAs Theranostics, 2017;7(6):1407-1421. 2017 [PMID: 28529626] (Bioassay, Human) Bioassay Human
Show All 183 Publications.

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Bioinformatics

Gene Symbol CSF2
Entrez
Uniprot