Recombinant Human GM-CSF Protein, CF

Images

 
1 μg/lane of Recombinant Human GM-CSF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human GM-CSF (Catalog # 215-IL/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 6-30 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Catalog# & Formulation Size Price

Recombinant Human GM-CSF Protein, CF Summary

Additional Information
A New and Improved rhGM-CSF is Now Available! It has lower endotoxin and is CHO expressed!
New Product 7954-GM/CF
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 6-30 pg/mL.
The specific activity of Recombinant Human GM-CSF is approximately 1.5 x 104 IU/μg, which is calibrated against human GM-CSF WHO International Standard (NIBSC code: 88/646).
Source
E. coli-derived human GM-CSF protein
Ala18-Glu144
Accession #
N-terminal Sequence
Ala18
Protein/Peptide Type
Recombinant Proteins
Gene
CSF2
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
215-GM/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GM-CSF Protein, CF

  • colony stimulating factor 2 (granulocyte-macrophage)
  • Colony-stimulating factor
  • CSF
  • CSF2
  • GMCSF
  • GM-CSF
  • GMCSFgranulocyte-macrophage colony-stimulating factor
  • granulocyte-macrophage colony stimulating factor
  • MGC131935
  • MGC138897
  • molgramostin
  • sargramostim

Background

GM‑CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte‑macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM‑CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM‑CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM‑CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy‑induced neutropenia, and GM‑CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM‑CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled
alpha -helices (8‑12). Mature human GM‑CSF shares 63%‑70% amino acid sequence identity with canine, feline, porcine, and rat GM‑CSF and 54% with mouse GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta  chain (CD131) which is also a component of the high‑affinity receptors for IL‑3 and IL‑5 (13, 14). In addition, GM‑CSF binds a naturally occurring soluble form of GM‑CSF R alpha (15). Human GM‑CSF is active on canine and feline cells but not on murine cells (16‑18).
  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
  7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
  8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
  9. Diederichs, K. et al. (1991) Science 254:1779. 
  10. Cantrell, M.A. et al. (1985) Proc. Natl. Acad. Sci. 82:6250.
  11. Lee, F. et al. (1985) Proc. Natl. Acad. Sci. 82:4360.
  12. Wong, G.G. et al. (1985) Science 228:810.
  13. Onetto-Pothier, N. et al. (1990) Blood 75:59.
  14. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
  15. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  16. Hogge, G.S. et al. (1990) Cancer Gene Ther. 6:26.
  17. Sprague, W.S. et al. (2005) J. Comp. Pathol. 133:136.
  18. Shanafelt, A.B. et al. (1991) J. Biol. Chem. 266:13804.

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215-GM/CF
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Publications for GM-CSF (215-GM/CF)(197)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 5 applications: Bioassay, Cell Culture Substrate, Ctrl, In Vivo, cell culture.


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Bioassay
(189)
Cell Culture Substrate
(2)
Ctrl
(1)
In Vivo
(2)
cell culture
(3)
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Human
(187)
Mouse
(2)
Rat
(2)
Canine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(3)
Rabbit
(1)
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Showing Publications 1 - 10 of 197. Show All 197 Publications.
Publications using 215-GM/CF Applications Species
X Chen, S Hao, Z Zhao, J Liu, Q Shao, F Wang, D Sun, Y He, W Gao, H Mao Interleukin 35: Inhibitory regulator in monocyte-derived dendritic cell maturation and activation Cytokine, 2018;108(0):43-52. 2018 [PMID: 29571039] (Bioassay, Human) Bioassay Human
T Kato, T Matsuda, Y Ikeda, JH Park, M Leisegang, S Yoshimura, T Hikichi, M Harada, M Zewde, S Sato, K Hasegawa, K Kiyotani, Y Nakamura Effective screening of T cells recognizing neoantigens and construction of T-cell receptor-engineered T cells Oncotarget, 2018;9(13):11009-11019. 2018 [PMID: 29541393] (Bioassay, Human) Bioassay Human
HD Chae, N Cox, GV Dahl, NJ Lacayo, KL Davis, S Capolicchi, M Smith, KM Sakamoto Niclosamide suppresses acute myeloid leukemia cell proliferation through inhibition of CREB-dependent signaling pathways Oncotarget, 2018;9(4):4301-4317. 2018 [PMID: 29435104] (Bioassay, Human) Bioassay Human
D Wang, XF Huang, B Hong, XT Song, L Hu, M Jiang, B Zhang, H Ning, Y Li, C Xu, X Lou, B Li, Z Yu, J Hu, J Chen, F Yang, H Gao, G Ding, L Liao, L Rollins, L Jones, SY Chen, H Chen Efficacy of intracellular immune checkpoint-silenced DC vaccine JCI Insight, 2018;3(3):. 2018 [PMID: 29415891] (Bioassay, Human) Bioassay Human
YJ Choi, SJ Park, YS Park, HS Park, KM Yang, K Heo EpCAM peptide-primed dendritic cell vaccination confers significant anti-tumor immunity in hepatocellular carcinoma cells PLoS ONE, 2018;13(1):e0190638. 2018 [PMID: 29298343] (Bioassay, Human) Bioassay Human
C Ge, R Li, H Song, T Geng, J Yang, Q Tan, L Song, Y Wang, Y Xue, Z Li, S Dong, Z Zhang, N Zhang, J Guo, L Hua, S Chen, X Song Phase I clinical trial of a novel autologous modified-DC vaccine in patients with resected NSCLC BMC Cancer, 2017;17(1):884. 2017 [PMID: 29268708] (Bioassay, Human) Bioassay Human
M Ohgidani, TA Kato, M Hosoi, M Tsuda, K Hayakawa, C Hayaki, R Iwaki, N Sagata, R Hashimoto, K Inoue, N Sudo, S Kanba Fibromyalgia and microglial TNF-?: Translational research using human blood induced microglia-like cells Sci Rep, 2017;7(1):11882. 2017 [PMID: 28928366] (Bioassay, Human) Bioassay Human
M Yoshida, Y Ishioka, T Ozawa, H Okuyama, M Iguchi, T Ota, T Ito, M Nagira, A Morita, H Tanaka, H Naito, H Kidoya, N Takakura Soluble HLA-associated peptide from PSF1 has a cancer vaccine potency Sci Rep, 2017;7(1):11137. 2017 [PMID: 28894200] (Bioassay, Human) Bioassay Human
H Nishi, K Furuhashi, X Cullere, G Saggu, MJ Miller, Y Chen, F Rosetti, SL Hamilton, L Yang, SP Pittman, J Liao, JM Herter, JC Berry, DJ DeAngelo, C Zhu, GC Tsokos, TN Mayadas Neutrophil Fc?RIIA promotes IgG-mediated glomerular neutrophil capture via Abl/Src kinases J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28891817] (Bioassay, Human) Bioassay Human
M Matsushita, K Ozawa, T Suzuki, M Nakamura, N Nakano, S Kanchi, D Ichikawa, E Matsuki, M Sakurai, D Karigane, H Kasahara, N Tsukamoto, T Shimizu, T Mori, H Nakajima, S Okamoto, Y Kawakami, Y Hattori CXorf48 is a potential therapeutic target for achieving treatment-free remission in CML patients Blood Cancer J, 2017;7(9):e601. 2017 [PMID: 28862699] (Bioassay, Human) Bioassay Human
Show All 197 Publications.

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Bioinformatics

Gene Symbol CSF2
Entrez
Uniprot