Recombinant Human GFR alpha-1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human GFR alpha-1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GDNF (Catalog # 212-GD) at 1 µg/mL binds Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera with an apparent Kd <2 nM.
Source
Mouse myeloma cell line, NS0-derived human GFR alpha-1/GDNF R alpha-1 protein
Human GFR alpha -1
(Ala19-Lys429)
Accession # NP_665736
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala19
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
GFRA1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
73.3 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
88-95 kDa, reducing conditions
Publications
Read Publications using
714-GR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GFR alpha-1 Fc Chimera Protein, CF

  • DKFZp313E1012
  • DKFZp686J0156
  • GDNF family receptor alpha 1
  • GDNF family receptor alpha-1
  • GDNF R alpha-1
  • GDNF receptor alpha-1
  • GDNFRAGFR-ALPHA-1
  • GDNFR-alpha-1
  • GDNFRMGC23045
  • GFR alpha1
  • GFR alpha-1
  • GFRA1
  • GFRa-1
  • GFR-alpha-1
  • Glial cell line-derived neurotrophic factor receptor alpha
  • GPI-linked anchor protein
  • PI-linked cell-surface accessory protein
  • RET ligand 1
  • RET1L
  • RETL1FLJ10561
  • TGF-beta related neurotrophic factor receptor 1
  • TGF-beta-related neurotrophic factor receptor 1
  • TRNR1FLJ31546

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), artemin and persephin are distant members of the TGF-beta  superfamily. They function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits [GDNF receptor alpha -1 (GFR alpha -1) or GFR alpha -2]. GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane‑associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase.

Human GFR alpha -1 cDNA encodes a 465 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% aa identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

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714-GR
Species: Hu
Applications: Binding Activity

Publications for GFR alpha-1/GDNF R alpha-1 (714-GR)(9)

We have publications tested in 4 confirmed species: Human, Rat, Bacteria, Chicken.

We have publications tested in 3 applications: Bioassay, ELISA development, PcIP.


Filter By Application
Bioassay
(7)
ELISA development
(1)
PcIP
(1)
All Applications
Filter By Species
Human
(5)
Rat
(2)
Bacteria
(1)
Chicken
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using 714-GR Applications Species
K Ban, S Feng, L Shao, M Ittmann RET signaling in prostate cancer Clin. Cancer Res., 2017;0(0):. 2017 [PMID: 28490466] (Bioassay, Human) Bioassay Human
Meka D, Muller-Rischart A, Nidadavolu P, Mohammadi B, Motori E, Ponna S, Aboutalebi H, Bassal M, Annamneedi A, Finckh B, Miesbauer M, Rotermund N, Lohr C, Tatzelt J, Winklhofer K, Kramer E Parkin cooperates with GDNF/RET signaling to prevent dopaminergic neuron degeneration. J Clin Invest, 2015;125(5):1873-85. 2015 [PMID: 25822020] (Bioassay, Human) Bioassay Human
Klein P, Muller-Rischart A, Motori E, Schonbauer C, Schnorrer F, Winklhofer K, Klein R Ret rescues mitochondrial morphology and muscle degeneration of Drosophila Pink1 mutants. EMBO J, 2014;33(4):341-55. 2014 [PMID: 24473149] (Bioassay, Human) Bioassay Human
Gatto G, Dudanova I, Suetterlin P, Davies A, Drescher U, Bixby J, Klein R Protein tyrosine phosphatase receptor type O inhibits trigeminal axon growth and branching by repressing TrkB and Ret signaling. J Neurosci, 2013;33(12):5399-410. 2013 [PMID: 23516305] (Bioassay, Human) Bioassay Human
Lundgren TK, Nakahata K, Fritz N, Rebellato P, Zhang S, Uhlen P RET PLC-gamma phosphotyrosine binding domain regulates Ca2+ signaling and neocortical neuronal migration. PLoS ONE, 2012;7(2):e31258. 2012 [PMID: 22355350] (Bioassay, Human) Bioassay Human
Lu B, PereiraPerrin M A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 2008;76(8):3530-8. 2008 [PMID: 18541656] (PcIP, Bacteria) PcIP Bacteria
Tatsumi N, Miki R, Katsu K, Yokouchi Y Neurturin-GFRalpha2 signaling controls liver bud migration along the ductus venosus in the chick embryo. Dev. Biol., 2007;307(1):14-28. 2007 [PMID: 17509555] (Bioassay, Chicken) Bioassay Chicken
Murase S, McKay RD A specific survival response in dopamine neurons at most risk in Parkinson&#039;s disease. J. Neurosci., 2006;26(38):9750-60. 2006 [PMID: 16988046] (Bioassay, Rat) Bioassay Rat
Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin. Biochemistry, 2005;44(7):2545-54. 2005 [PMID: 15709767] (ELISA development, Rat) ELISA development Rat

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Bioinformatics

Gene Symbol GFRA1
Uniprot