GFR alpha-1/GDNF R alpha-1: Novus Biologicals

AF560

GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

(8 Reviews)

100 μg

AF560

HAF109). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

GFRa-1/GDNF Ra-1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

<b>Biological Strategies Validation. </b>Ectopic RAR gamma expression by GFR alpha 1+ spermatogonia. (A) The CAG-CAT-3xFLAG-Rarg transgene. When CAT between the loxP sites is deleted by TM-activated Cre, FLAG-tagged RAR gamma is constitutively expressed under the control of the CAG promoter. (B) Experimental design of the fate analysis of GFR alpha 1+ cells with enforced FLAG-RAR gamma expression upon VA readministration in VAD mice, as shown in C-F. Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg transgenic mice were maintained in VAD and VA was administered 2 days after TM injection, as indicated. Testes were then processed for IF. (C,D) IF images of whole-mount seminiferous tubules of the mice described above, 2 days after VA injection, stained for FLAG-RAR gamma (green) and KIT (magenta) (C), and cell number relative to the number of initial induced cells (D). Data for GFP-labeled NGN3+ and GFR alpha 1+ cells are reproduced from Fig. 2C and Fig. 3C, respectively, for comparison. The mean±s.e.m. value of three testes is shown. *P<0.003 (t-test), compared with the values of FLAG-RAR gamma + GFR alpha 1+ cells at day 2. (E,F) Representative confocal images of the same field of whole-mounts of seminiferous tubules of mice treated as described above, at 2 days after VA injection; staining was performed for GFR alpha 1, KIT and FLAG (E). Open arrowheads, white arrowheads and small arrows indicate FLAG+ cells that are GFR alpha 1+/KIT+, GFR alpha 1+/KIT− and GFR alpha 1−/KIT+, respectively. (F) Quantitation of GFP+ and FLAG-RAR gamma + cells showing different patterns of GFR alpha 1 and KIT expression in Gfra1-CreERT2; CAG-CAT-EGFP and Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg mice, respectively. Cell numbers are shown above each bar. (G) Experimental design of the fate analysis of GFR alpha 1+ cells with enforced FLAG-RAR gamma expression under normal conditions, as shown in H-J. Gfra1-CreERT2; CAG-CAT-3xFLAG-Rarg transgenic mice were pulsed with TM at 13-17 weeks of age, and after 2 and 10 days their testes were processed for IF. (H) IF images of whole-mount seminiferous tubules 2 and 10 days after TM injection, stained f

Fate of pulse-labeled GFR alpha 1+ spermatogonia during VAD/VA administration. (A) The experimental schedule for B and C. Two days after the TM pulse, Gfra1-CreERT2; CAG-CAT-EGFP transgenic mice maintained in VAD were injected with VA and then fed a normal diet. Testis samples were harvested at the indicated times. (B) Representative IF images of whole-mount seminiferous tubule 0, 2, 6 and 10 days after VA injection stained for GFP and GFR alpha 1. (C) The number of GFP-labeled GFR alpha 1+ Aundiff (magenta), GFR alpha 1− Aundiff (green), KIT+ (blue) spermatogonia and total labeled (black) cells. Shown is the mean±s.e.m. of five, five, seven, six, four and three testes for days 0, 2, 4, 6, 8 and 10, respectively. Data, except for those of GFR alpha 1+ cells, on days 2, 6, 8 and 10 were significantly different compared with the values on day 0 (P<0.03, t-test). (D) Schedule for E and F. After the TM pulse, Gfra1-CreERT2; CAG-CAT-EGFP transgenic mice were continually fed the VAD diet. (E) Representative IF images of whole-mount seminiferous tubule 2, 14 and 30 days after TM pulse stained for GFP and GFR alpha 1. (F) Number of GFP-labeled GFR alpha 1+ Aundiff (magenta) and GFR alpha 1− Aundiff (green) spermatogonia and total labeled cells (black), shown as the mean±s.e.m. of eight, three and five testes on days 0, 14 and 30, respectively. *P=0.032, **P<0.002 (t-test) compared with the values for day 2. Scale bars: 50 μm. Image collected and cropped by CiteAb from the following open publication (//journals.biologists.com/dev/article/doi/10.1242/dev.118695/258623/Hierarchical-differentiation-competence-in), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Characterization of GFR alpha 1 and co-receptor expression in neurons and synapses of the septum→Hb→IPN pathway.(A) dTomato epifluorescence (red) in sagittal sections of Gfra1dTOM mouse brain injected with tamoxifen at 3 months counterstained with DAPI (blue). Scale bar, 500 μm. (B) GFP (green) and ChAT (red) immunostaining in sagittal sections of a 3-month-old Gfra1GFP mouse brain counterstained with DAPI (blue). Scale bar, 400 μm. (C, D) GFP (green), ChAT (red), and SP (gray) immunostaining in coronal sections of the mHb (C) and IPN (D) of a 3-month-old Gfra1GFP mouse brain. Scale bars, 200 μm. (E) GFR alpha 1 immunostaining (red) in coronal sections of the mHb and IPN from a 3-month-old control (C57BL6/J) mouse. Scale bars, 200 μm. (F) GFP (green) and GFR alpha 1 (red) immunostaining in a coronal section containing the IPN of a 3-month-old Gad67Cre;R1CGfx/fx mouse. GFP signal represents the GABAergic cells that express Gfra1. Scale bars, 100 μm (merged) and 35 μm (inset). (G) GFP (green) and CR (red) immunostaining in coronal sections of the BAC and TS from a 3-month-old Gfra1GFP mouse brain counterstained with DAPI (blue). Scale bars, 100 μm (BAC) and 200 μm (TS). (H, I) Immunoblots of We, Ct, and Sn protein extracts from the mHb and IPN of 3-month-old C57BL6/J mice probed for GFR alpha 1 and NCAM (H) or RET and PSD95 (I). Tubulin was probed as loading control. (J, M) GFR alpha 1 (green) and PSD93 (red) immunostaining in the mHb (J) and IPN (L) of 3-month-old C57BL6/J mice. Scale bars, 10 μm (merged) and 5 μm (insets). Graphs show the quantification (± SEM) of GFR alpha 1, PSD93, and double-labeled puncta in mHb (K) and IPN (M). N = 5 mice (25–30 images per mouse per structure). The data underlying this figure can be found at //figshare.com/projects/Raw_Data_Fernandez-Suarez_et_al_2021/123406. 3V, 3th ventricle; ChAT, choline acetyltransferase; CR, calretinin; Ct, cytosolic protein fraction; dIPN, dorsal interpeduncular nucleus; dmHb, dorsal medial habenula; FR, fasciculus retroflexus; GFR alpha 1$

Spermatogenesis development of adult Mafb-cKO mice.Six-week-old mice were injected with tamoxifen and testes of cKO mice at 3- and 8-months of age were examined compared to those of age-matched controls (n = 4 for each group). (A) Testicular sections stained with Periodic acid-Schiff (PAS). (B) Immunostaining with various testicular cell types; undifferentiated spermatogonia (GFRA1 and PLZF), differentiated spermatogonia (KIT+ inside the tubule), spermatocytes (SCP3), spermatids (PNA-Lectin), Sertoli cells (SOX9 and Vimentin), and Leydig cells (KIT+ outside the tubule and STAR). The color of markers is indicated in the left boxes in panels. Colored arrows corresponding to the boxed markers are shown. (C) The expression level of the genes involved in testis function and germ cell development. The marker genes representing undifferentiated spermatogonia (Nanos3), differentiated spermatogonia (c-Kit), undifferentiated and differentiated spermatogonia (Sohlh1), differentiated spermatogonia and preleptotene spermatocytes (Stra8), spermatids (Prm2), Leydig cells (Hsd3b1), and Sertoli cells (Sox9) were analyzed by qRT-PCR. Each reaction was performed in duplicate for each gene. The data represent the means±SEM and are shown as relative mRNA expression after normalization to Hprt. *P<0.05. (D) The proportion of seminiferous stages: I-III, IV-VI, VII-VIII, and IX-XII from each genotype. *P<0.05. M; Month. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0190800), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Expression of genes in the RA signaling pathway and the specificity of RAR gamma expression by NGN3+ spermatogonia. (A) Scatter plot comparing the levels of transcripts expressed by GFR alpha 1+ and NGN3+ cells according to microarray analysis. For each fraction, average values from three independent RNA samples sorted from different animals are shown. The middle line indicates a difference of 0, and the outer lines represent ratios {log2([NGN3+]/[GFR alpha 1+])} of 1.0 and −1.0. Red dots indicate genes expressed at significantly different levels (P<0.05, t-test with Benjamini–Hochberg correction). Members of the Rar and Rxr gene families are indicated. A list of the genes in this panel is shown in supplementary material Table S2. (B) qRT-PCR analysis of Rara and Rarg mRNA expression in GFR alpha 1+, NGN3+ and KIT+ spermatogonia. Relative copy numbers are shown in arbitrary units after normalization to the amount of Actb mRNA. The mean±s.e.m. values of three independent cell preparations from different animals are shown. *P=0.043, **P=0.011 and ***P=0.0065 (t-test). (C) Representative IF images of whole-mount seminiferous tubules from Ngn3-EGFP mice stained for EGFP, GFR alpha 1 and RAR gamma . (D,E) Frequency of single- and double-positive spermatogonia for the expression of RAR gamma and NGN3 (recognized as RAR gamma +/KIT− and NGN3-EGFP+/KIT−, respectively) (D) and of RAR gamma and GFR alpha 1 (E). Total counts are shown above each bar. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (//journals.biologists.com/dev/article/doi/10.1242/dev.118695/258623/Hierarchical-differentiation-competence-in), licensed under a CC-BY license. Not internally tested by R&D Systems.$

<b>Biological Strategies Validation. </b>Retention of the spermatogonial phenotype following gene correction.Immunostaining was performed on gene-corrected GT59 (left) and GT65 cells (right): DAZL, a germ cell specific marker; GFRA1, POU5F1, ETV5, CDH1, and SOHLH1, markers of undifferentiated spermatogonia. Additionally, GT59 and GT65 cells were treated with the differentiation factor, retinoic acid (1 µM) or a vehicle control and then immunostained to examine levels of ZBTB16, a marker of undifferentiated spermatogonia. Bar represents 50 microns. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25409432), licensed under a CC-BY license. Not internally tested by R&D Systems.

Testis anatomy and spermatogonial populations and their kinetics in the VAD model. (A) Anatomy of seminiferous tubules and seminiferous epithelium. Aundiff spermatogonia, including GFR alpha 1+ (magenta) and NGN3+ (green) cells and KIT+ differentiating spermatogonia (blue), reside in the basal compartment (between the basement membrane and the tight junction of Sertoli cells). (B) Hierarchical and heterogeneous composition of spermatogonia. PL, preleptotene spermatocytes. (C) Representative whole-mount IF images of spermatogonia derived from an Ngn3-EGFP mouse triple stained for GFR alpha 1, GFP and KIT. (D) The experimental schedule for E and F. Wild-type VAD mice were injected with VA and fed a normal (VA-sufficient) diet thereafter, before analysis at the indicated times. (E) Representative images of in situ hybridization analysis of Gfra1, Ngn3 and Kit expression in testis sections. Arrowheads indicate spermatogonia expressing these genes. Note the persisting Kit expression in interstitial cells (asterisks). (F) Counts of Gfra1+, Ngn3+ and Kit+ spermatogonia. Raw counts are summarized in supplementary material Table S1. Scale bars: 100 μm. Image collected and cropped by CiteAb from the following open publication (//journals.biologists.com/dev/article/doi/10.1242/dev.118695/258623/Hierarchical-differentiation-competence-in), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF560U-100UG
Alexa Fluor 405	AF560V-100UG
Alexa Fluor 488	AF560G-100UG
Alexa Fluor 532	AF560X-100UG
Alexa Fluor 594	AF560T-100UG
Alexa Fluor 647	AF560R-100UG
Alexa Fluor 700	AF560N-100UG
Alexa Fluor 750	AF560S-100UG
Alexa Fluor Plus 405	AF560AFP405-100UG
Alexa Fluor Plus 488	AF560AFP488-100UG
Alexa Fluor Plus 555	AF560AFP555-100UG
Alexa Fluor Plus 594	AF560AFP594-100UG
Alexa Fluor Plus 647	AF560AFP647-100UG
Alexa Fluor Plus 680	AF560AFP680-100UG
Allophycocyanin	AF560A
Biotin	BAF560
CoraFluor 1	AF560CL1
DyLight 350	AF560D
DyLight 405	AF560E
DyLight 488	AF560K
DyLight 550	AF560L
DyLight 594	AF560M
DyLight 650	AF560W
DyLight 680	AF560Y
DyLight 755	AF560Z
FITC	AF560F
HRP	AF560H
Janelia Fluor 525	AF560JF525
Janelia Fluor 549	AF560I
Janelia Fluor 646	AF560J
Janelia Fluor 669	AF560JF669
PE	AF560P
PerCP	AF560C
mFluor Violet 450 SE	AF560MFV450
mFluor Violet 500 SE	AF560MFV500
mFluor Violet 610 SE	AF560MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF560-SP

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560-GR

Recombinant Rat GFR alpha-1 Fc Chimera Protein

(17 Publications)

100 μg

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

,

Applications: ,

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	560-GR-100/CF

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AF714

GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

(3 Reviews)

(11 Publications)

100 μg

AF714

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to ventral horn motoneurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

GFRa-1/GDNF Ra-1 was detected in immersion fixed paraffin-embedded sections of human spinal cord using Goat Anti-Human GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF714) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to ventral horn motoneurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="GFRa-1/GDNF Ra-1 was detected in immersion fixed paraffin-embedded sections of human spinal cord using Goat Anti-Human GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF714) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to ventral horn motoneurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF714U-100UG
Alexa Fluor 405	AF714V-100UG
Alexa Fluor 488	AF714G-100UG
Alexa Fluor 532	AF714X-100UG
Alexa Fluor 594	AF714T-100UG
Alexa Fluor 647	AF714R-100UG
Alexa Fluor 700	AF714N-100UG
Alexa Fluor 750	AF714S-100UG
Alexa Fluor Plus 405	AF714AFP405-100UG
Alexa Fluor Plus 488	AF714AFP488-100UG
Alexa Fluor Plus 555	AF714AFP555-100UG
Alexa Fluor Plus 594	AF714AFP594-100UG
Alexa Fluor Plus 647	AF714AFP647-100UG
Alexa Fluor Plus 680	AF714AFP680-100UG
Allophycocyanin	AF714A
Biotin	BAF714
CoraFluor 1	AF714CL1
DyLight 350	AF714D
DyLight 405	AF714E
DyLight 488	AF714K
DyLight 550	AF714L
DyLight 594	AF714M
DyLight 650	AF714W
DyLight 680	AF714Y
DyLight 755	AF714Z
FITC	AF714F
HRP	AF714H
Janelia Fluor 525	AF714JF525
Janelia Fluor 549	AF714I
Janelia Fluor 646	AF714J
Janelia Fluor 669	AF714JF669
PE	AF714P
PerCP	AF714C
mFluor Violet 450 SE	AF714MFV450
mFluor Violet 500 SE	AF714MFV500
mFluor Violet 610 SE	AF714MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF714-SP

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NBP1-77043

GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

(3 Publications)

0.1 mg

NBP1-77043

$Western Blot: GFR alpha-1/GDNF R alpha-1 Antibody [NBP1-77043] - Analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1 ug/mL$

Immunohistochemistry: GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free [NBP1-77043] - Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 ug/mL.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77043-0.025mg

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714-GR

Recombinant Human GFR alpha-1 Fc Chimera Protein, CF

(12 Publications)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB560

GFR alpha-1/GDNF R alpha-1 Antibody (81401) [Unconjugated]

(6 Publications)

500 μg

MAB560

HAF007). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions (recommended) and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of rat spinal cord tissue and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat GFRa-1/GDNF Ra-1 Monoclonal Antibody (Catalog # MAB560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions (recommended) and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat spinal cord tissue and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat GFRa-1/GDNF Ra-1 Monoclonal Antibody (Catalog # MAB560) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 60 kDa (as indicated). This experiment was conducted under non-reducing conditions (recommended) and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

GFRa-1/GDNF Ra-1 was detected in perfusion fixed frozen sections of rat spinal cord using Rat GFRa-1/GDNF Ra-1 Monoclonal Antibody (Catalog # MAB560) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB560U-100UG
Alexa Fluor 405	FAB560V-100UG
Alexa Fluor 488	FAB560G-100UG
Alexa Fluor 532	FAB560X-100UG
Alexa Fluor 594	FAB560T-100UG
Alexa Fluor 647	FAB560R-100UG
Alexa Fluor 700	FAB560N-100UG
Alexa Fluor 750	FAB560S-100UG
Alexa Fluor Plus 405	FAB560AFP405-100UG
Alexa Fluor Plus 488	FAB560AFP488-100UG
Alexa Fluor Plus 555	FAB560AFP555-100UG
Alexa Fluor Plus 594	FAB560AFP594-100UG
Alexa Fluor Plus 647	FAB560AFP647-100UG
Alexa Fluor Plus 680	FAB560AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB560-SP

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MAB7141

GFR alpha-1/GDNF R alpha-1 Antibody (260714) [Unconjugated]

(1 Review)

(3 Publications)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7141U-100UG
Alexa Fluor 405	FAB7141V-100UG
Alexa Fluor 488	FAB7141G-100UG
Alexa Fluor 532	FAB7141X-100UG
Alexa Fluor 594	FAB7141T-100UG
Alexa Fluor 647	FAB7141R-100UG
Alexa Fluor 700	FAB7141N-100UG
Alexa Fluor 750	FAB7141S-100UG
Alexa Fluor Plus 405	FAB7141AFP405-100UG
Alexa Fluor Plus 488	FAB7141AFP488-100UG
Alexa Fluor Plus 555	FAB7141AFP555-100UG
Alexa Fluor Plus 594	FAB7141AFP594-100UG
Alexa Fluor Plus 647	FAB7141AFP647-100UG
Alexa Fluor Plus 680	FAB7141AFP680-100UG
Allophycocyanin	FAB7141A
Biotin	FAB7141B
CoraFluor 1	FAB7141CL1
DyLight 350	FAB7141D
DyLight 405	FAB7141E
DyLight 488	FAB7141K
DyLight 550	FAB7141L
DyLight 594	FAB7141M
DyLight 650	FAB7141W
DyLight 680	FAB7141Y
DyLight 755	FAB7141Z
FITC	FAB7141F
HRP	FAB7141H
Janelia Fluor 525	FAB7141JF525
Janelia Fluor 549	FAB7141I
Janelia Fluor 646	FAB7141J
Janelia Fluor 669	FAB7141JF669
PE	FAB7141P
PerCP	FAB7141C
mFluor Violet 450 SE	FAB7141MFV450
mFluor Violet 500 SE	FAB7141MFV500
mFluor Violet 610 SE	FAB7141MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB7141-SP

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NBP2-14045

GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

0.1 ml

NBP2-14045

Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human smooth muscle shows strong granular cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human prostate shows strong granular cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human colon shows moderate granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: GFR alpha-1/GDNF R alpha-1 Antibody [NBP2-14045] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-14045-25ul

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NBP2-90463

GFR alpha-1/GDNF R alpha-1 Antibody (009) - Azide and BSA Free

100 ul

NBP2-90463

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-90463AF350
Alexa Fluor 405	NBP2-90463AF405
Alexa Fluor 488	NBP2-90463AF488
Alexa Fluor 532	NBP2-90463AF532
Alexa Fluor 594	NBP2-90463AF594
Alexa Fluor 647	NBP2-90463AF647
Alexa Fluor 700	NBP2-90463AF700
Alexa Fluor 750	NBP2-90463AF750
Alexa Fluor Plus 405	NBP2-90463AFP405
Alexa Fluor Plus 488	NBP2-90463AFP488
Alexa Fluor Plus 555	NBP2-90463AFP555
Alexa Fluor Plus 594	NBP2-90463AFP594
Alexa Fluor Plus 647	NBP2-90463AFP647
Alexa Fluor Plus 680	NBP2-90463AFP680
Allophycocyanin	NBP2-90463APC
Biotin	NBP2-90463B
CoraFluor 1	NBP2-90463CL1
DyLight 350	NBP2-90463UV
DyLight 405	NBP2-90463V
DyLight 488	NBP2-90463G
DyLight 550	NBP2-90463R
DyLight 594	NBP2-90463DL594
DyLight 650	NBP2-90463C
DyLight 680	NBP2-90463FR
DyLight 755	NBP2-90463IR
FITC	NBP2-90463F
HRP	NBP2-90463H
Janelia Fluor 525	NBP2-90463JF525
Janelia Fluor 549	NBP2-90463JF549
Janelia Fluor 646	NBP2-90463JF646
Janelia Fluor 669	NBP2-90463JF669
PE	NBP2-90463PE
PerCP	NBP2-90463PCP
mFluor Violet 450 SE	NBP2-90463MFV450
mFluor Violet 500 SE	NBP2-90463MFV500
mFluor Violet 610 SE	NBP2-90463MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-90463-50ul

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NBP2-11349

GFR alpha-1/GDNF R alpha-1 Overexpression Lysate

0.1 mg

NBP2-11349

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-05472

GFR alpha-1/GDNF R alpha-1 Overexpression Lysate

0.1 mg

NBP2-05472

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-10479

GFR alpha-1/GDNF R alpha-1 Overexpression Lysate

0.1 mg

NBP2-10479

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

GFRA1 Products By Gene Name

GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

Recombinant Rat GFR alpha-1 Fc Chimera Protein

GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]

GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

Recombinant Human GFR alpha-1 Fc Chimera Protein, CF

GFR alpha-1/GDNF R alpha-1 Antibody (81401) [Unconjugated]

GFR alpha-1/GDNF R alpha-1 Antibody (260714) [Unconjugated]

GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

GFR alpha-1/GDNF R alpha-1 Antibody (009) - Azide and BSA Free

GFR alpha-1/GDNF R alpha-1 Overexpression Lysate

GFR alpha-1/GDNF R alpha-1 Overexpression Lysate

GFR alpha-1/GDNF R alpha-1 Overexpression Lysate