Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Images

 
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.
iPSC-derived cerebral organoids (day 45) were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and brain organoid culture medium, which includes Recombinant Human FGF-basic (Catalog # 3718-FB) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations

Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.
Source
E. coli-derived human FGF basic/FGF2/bFGF protein
Ala144-Ser288
Accession #
N-terminal Sequence
Ala144
Protein/Peptide Type
Recombinant Proteins
Gene
FGF2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
3718-FB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • HBGF-2
  • heparin-binding growth factor 2
  • Prostatropin

Background

FGF basic (also known as FGF-2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF-2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4-6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).

  1. Sorenson, V. et al. (2006) BioEssays 28:504.
  2. Kardami, E. et al. (2004) Cardiovasc. Res. 63:458.
  3. Nugent, M.A. and R.V. Lozzo (2000) Int. J. Biochem. Cell Biol. 32:115.
  4. Abraham, J.A. et al. (1986) EMBO J. 5:2523.
  5. Prats, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:1836.
  6. Arnaud, E. et al. (1999) Mol. Cell. Biol. 19:505.
  7. Foletti, A. et al. (2003) Cell. Mol. Life Sci. 60:2254.
  8. Arese, M. et al. (1999) Mol. Biol. Cell 10:1429.
  9. Pintucci, G. et al. (1996) Mol. Biol. Cell 7:1249.
  10. Nickel, W. (2005) Traffic 6:607.
  11. SwissProt # P09038.
  12. Klagsbrun, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1839.
  13. Bailly, K. et al. (2000) FASEB J. 14:333.
  14. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.

Customers Who Viewed This Item Also Viewed...

DVE00
Species: Hu
Applications: ELISA
236-EG
Species: Hu
Applications: BA
232-FA/CF
Species: Hu
Applications: BA
291-G1
Species: Hu
Applications: BA
NB600-1287
Species: Ca, Hu, Mu, Rb, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-00932
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
D6050
Species: Hu
Applications: ELISA
210-TA
Species: Hu
Applications: BA
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
294-HG/CF
Species: Hu
Applications: BA
DBD00
Species: Hu
Applications: ELISA
251-KG/CF
Species: Hu
Applications: BA
NB300-141
Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
267-N3/CF
Species: Hu
Applications: BA
256-GF/CF
Species: Hu
Applications: BA
3718-FB
Species: Hu
Applications: Bioactivity

Publications for FGF basic/FGF2/bFGF (3718-FB)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 3 applications: Bioassay, Cell Culture, ELISA Capture.


Filter By Application
Bioassay
(10)
Cell Culture
(1)
ELISA Capture
(1)
All Applications
Filter By Species
Human
(10)
Mouse
(1)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 3718-FB Applications Species
Zhang Q, Chen L, Huang L, Cheng H, Wang L, Xu L, Hu D, He C, Fu C, Wei Q CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction. Mol Med, 0;28(1):145. 0 [PMID: 36463112] (Bioassay, Human) Bioassay Human
JA Watts, C Grunseich, Y Rodriguez, Y Liu, D Li, JT Burdick, A Bruzel, RJ Crouch, RW Mahley, SH Wilson, VG Cheung A common transcriptional mechanism involving R-loop and RNA abasic site regulates an enhancer RNA of APOE Nucleic Acids Research, 2022;0(0):. 2022 [PMID: 36453989] (Bioassay, Human) Bioassay Human
Tangeman J, Perez-Estrada J, Van Zeeland E, Liu L, Danciutiu A, Grajales-Esquivel E, Smucker B, Liang C, Del Rio-Tsonis K A Stage-Specific OTX2 Regulatory Network and Maturation-Associated Gene Programs Are Inherent Barriers to RPE Neural Competency. Front Cell Dev Biol, 0;10(0):875155. 0 [PMID: 35517508] (Bioassay, Chicken) Bioassay Chicken
F Bonini, S Mosser, FM Mor, A Boutabla, P Burch, A Béduer, A Roux, T Braschler The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial Integration Brain sciences, 2022;12(4):. 2022 [PMID: 35447953] (Bioassay, Human) Bioassay Human
Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M Physical and functional interactome atlas of human receptor tyrosine kinases. EMBO Rep, 0;23(6):e54041. 0 [PMID: 35384245] (Bioassay, Human) Bioassay Human
HC Ting, HI Yang, HJ Harn, IM Chiu, HL Su, X Li, MF Chen, TJ Ho, CA Liu, YJ Tsai, TW Chiou, SZ Lin, CY Chang Coactivation of GSK3beta and IGF-1 Attenuates Amyotrophic Lateral Sclerosis Nerve Fiber Cytopathies in SOD1 Mutant Patient-Derived Motor Neurons Cells, 2021;10(10):. 2021 [PMID: 34685754] (Bioassay, Human) Bioassay Human
AN Holowatyj, B Gigic, CA Warby, J Ose, T Lin, P Schrotz-Ki, CM Ulrich, JJ Bernard The Use of Human Serum Samples to Study Malignant Transformation: A Pilot Study Cells, 2021;10(10):. 2021 [PMID: 34685650] (Bioassay, Human) Bioassay Human
Kim Y, Kim H, Jung D, Kang D, Nam D, Nam H, Cho H Glioblastoma patient-derived cell-based phenotypic drug screening and identification of possible action mechanisms through proteomic analysis. STAR Protoc, 0;2(4):100849. 0 [PMID: 34611628] (Bioassay, Human) Bioassay Human
M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan Regulation of prefrontal patterning and connectivity by retinoic acid Nature, 2021;0(0):. 2021 [PMID: 34599305] (Cell Culture, Human) Cell Culture Human
S Jadhav, S Tripathi, A Chandrekar, SS Waikar, LL Hsiao A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma PLoS ONE, 2021;16(1):e0245614. 2021 [PMID: 33481901] (ELISA Capture, Human) ELISA Capture Human
Show All 12 Publications.

Reviews for FGF basic/FGF2/bFGF (3718-FB) (0)

There are no reviews for FGF basic/FGF2/bFGF (3718-FB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for FGF basic/FGF2/bFGF (3718-FB). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Additional FGF basic/FGF2/bFGF Products

Bioinformatics Tool for FGF basic/FGF2/bFGF (3718-FB)

Discover related pathways, diseases and genes to FGF basic/FGF2/bFGF (3718-FB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FGF basic/FGF2/bFGF (3718-FB)

Discover more about diseases related to FGF basic/FGF2/bFGF (3718-FB).
 

Pathways for FGF basic/FGF2/bFGF (3718-FB)

View related products by pathway.

PTMs for FGF basic/FGF2/bFGF (3718-FB)

Learn more about PTMs related to FGF basic/FGF2/bFGF (3718-FB).

Blogs on FGF basic/FGF2/bFGF

There are no specific blogs for FGF basic/FGF2/bFGF, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol FGF2
Uniprot