Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.

iPSC-derived cerebral organoids (day 45) were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and brain organoid culture medium, which includes Recombinant Human FGF-basic (Catalog # 3718-FB) and Recombinant Human Noggin (6057-NG), along with the other reagents listed in the brain organoid culture recipe. Cerebral organoids were stained for Syto6 (blue), Pax6 (red), and Vimentin (green). (A) Image taken at 4x magnification. (B) An enlarged view of the area shown within the white box in part A of the figure. (C) Image taken at 15x magnification. Images courtesy of LifeCanvas Technologies.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 0.1-0.6 ng/mL.
• Source: E. coli-derived human FGF basic/FGF2/bFGF protein
  Ala144-Ser288
• Accession #: NP_001997
• N-terminal Sequence: Ala144
• Protein/Peptide Type: Recombinant Proteins
• Gene: FGF2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF basic/FGF2/bFGF (145 aa) Protein, CF

• basic fibroblast growth factor bFGF
• Basic fibroblast growth factor
• bFGF
• FGF basic
• FGF2
• FGF-2
• FGFBprostatropin
• fibroblast growth factor 2 (basic)
• HBGF-2
• heparin-binding growth factor 2
• Prostatropin

Background

FGF basic (also known as FGF-2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF-1) and FGF basic (FGF-2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4-6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).

1. Sorenson, V. et al. (2006) BioEssays 28:504.
2. Kardami, E. et al. (2004) Cardiovasc. Res. 63:458.
3. Nugent, M.A. and R.V. Lozzo (2000) Int. J. Biochem. Cell Biol. 32:115.
4. Abraham, J.A. et al. (1986) EMBO J. 5:2523.
5. Prats, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:1836.
6. Arnaud, E. et al. (1999) Mol. Cell. Biol. 19:505.
7. Foletti, A. et al. (2003) Cell. Mol. Life Sci. 60:2254.
8. Arese, M. et al. (1999) Mol. Biol. Cell 10:1429.
9. Pintucci, G. et al. (1996) Mol. Biol. Cell 7:1249.
10. Nickel, W. (2005) Traffic 6:607.
11. SwissProt # P09038.
12. Klagsbrun, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1839.
13. Bailly, K. et al. (2000) FASEB J. 14:333.
14. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.

Publications for FGF basic/FGF2/bFGF (3718-FB)(12)

Publications using 3718-FB

• Zhang Q, Chen L, Huang L, Cheng H, Wang L, Xu L, Hu D, He C, Fu C, Wei Q CD44 promotes angiogenesis in myocardial infarction through regulating plasma exosome uptake and further enhancing FGFR2 signaling transduction. Mol Med, 0;28(1):145. 0 [PMID: 36463112] (Bioassay, Human)
• JA Watts, C Grunseich, Y Rodriguez, Y Liu, D Li, JT Burdick, A Bruzel, RJ Crouch, RW Mahley, SH Wilson, VG Cheung A common transcriptional mechanism involving R-loop and RNA abasic site regulates an enhancer RNA of APOE Nucleic Acids Research, 2022;0(0):. 2022 [PMID: 36453989] (Bioassay, Human)
• Tangeman J, Perez-Estrada J, Van Zeeland E, Liu L, Danciutiu A, Grajales-Esquivel E, Smucker B, Liang C, Del Rio-Tsonis K A Stage-Specific OTX2 Regulatory Network and Maturation-Associated Gene Programs Are Inherent Barriers to RPE Neural Competency. Front Cell Dev Biol, 0;10(0):875155. 0 [PMID: 35517508] (Bioassay, Chicken)
• F Bonini, S Mosser, FM Mor, A Boutabla, P Burch, A Béduer, A Roux, T Braschler The Role of Interstitial Fluid Pressure in Cerebral Porous Biomaterial Integration Brain sciences, 2022;12(4):. 2022 [PMID: 35447953] (Bioassay, Human)
• Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M Physical and functional interactome atlas of human receptor tyrosine kinases. EMBO Rep, 0;23(6):e54041. 0 [PMID: 35384245] (Bioassay, Human)
• HC Ting, HI Yang, HJ Harn, IM Chiu, HL Su, X Li, MF Chen, TJ Ho, CA Liu, YJ Tsai, TW Chiou, SZ Lin, CY Chang Coactivation of GSK3beta and IGF-1 Attenuates Amyotrophic Lateral Sclerosis Nerve Fiber Cytopathies in SOD1 Mutant Patient-Derived Motor Neurons Cells, 2021;10(10):. 2021 [PMID: 34685754] (Bioassay, Human)
• AN Holowatyj, B Gigic, CA Warby, J Ose, T Lin, P Schrotz-Ki, CM Ulrich, JJ Bernard The Use of Human Serum Samples to Study Malignant Transformation: A Pilot Study Cells, 2021;10(10):. 2021 [PMID: 34685650] (Bioassay, Human)
• Kim Y, Kim H, Jung D, Kang D, Nam D, Nam H, Cho H Glioblastoma patient-derived cell-based phenotypic drug screening and identification of possible action mechanisms through proteomic analysis. STAR Protoc, 0;2(4):100849. 0 [PMID: 34611628] (Bioassay, Human)
• M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan Regulation of prefrontal patterning and connectivity by retinoic acid Nature, 2021;0(0):. 2021 [PMID: 34599305] (Cell Culture, Human)
• S Jadhav, S Tripathi, A Chandrekar, SS Waikar, LL Hsiao A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma PLoS ONE, 2021;16(1):e0245614. 2021 [PMID: 33481901] (ELISA Capture, Human)
• Linkous A, Fine H Generating Patient-Derived Gliomas within Cerebral Organoids. STAR Protoc, 0;1(1):. 0 [PMID: 33103125] (Bioassay, Human)
• E Steinberg, A Fluksman, C Zemmour, K Tischenko, A Karsch-Blu, Y Brill-Karn, AE Birsner, RJ D'Amato, O Benny Low dose amiodarone reduces tumor growth and angiogenesis Sci Rep, 2020;10(1):18034. 2020 [PMID: 33093573] (Bioassay, Mouse)

FAQs for FGF basic/FGF2/bFGF (3718-FB). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
   • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Bioinformatics

• Gene Symbol: FGF2
• Uniprot:
  • NP_001997()