Recombinant Human/Feline CXCL12/SDF-1 beta (aa 19-93), CF

• Reactivity: Hu, Fe
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human/Feline CXCL12/SDF-1 beta (aa 19-93), CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CXCR4. The ED₅₀ for this effect is 0.15-0.6 µg/mL.
• Source: E. coli-derived CXCL12/SDF-1 beta protein
  Ser19-Met93
• Accession #: BAA28602
• N-terminal Sequence: Ser19
• Protein/Peptide Type: Recombinant Proteins
• Gene: CXCL12
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 8.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Feline CXCL12/SDF-1 beta (aa 19-93), CF

• CXCL12/SDF-1 beta
• hSDF-1beta
• SDF 1beta
• SDF1 beta
• SDF1b

Background

CXCL12, also known as SCYB12, PBSF and SDF-1 beta , is an 8.3 kDa, heparin-binding member of the CXC (or alpha-) family of chemokines (1, 2). Feline CXCL12( beta ) is synthesized as a 93 amino acid (aa) precursor that contains a 21 aa signal sequence and a 72 aa mature region (3). The mature molecule exhibits a typical three antiparallel beta -strand chemokine-like fold. There are no potential N-linked glycosylation sites. N-terminal aa’s 1 - 8 form a receptor binding site, while aa’s 1 and 2 (Lys-Pro) are involved in receptor activation (4). The C-terminus is likely associated with heparin binding (5). SDF-1 beta circulates and undergoes proteolytic processing. CD26 will remove the first two N-terminal amino acids, possibly creating a reduced-activity chemokine (5, 6). In addition to the beta -isoform, alternate splicing of the feline SDF-1 gene generates an alpha -isoform. The alpha isoform is identical to SDF-1 beta , but shorter by four aa’s at the C-terminus (3). Although alpha - and beta -isoforms show similar activity, SDF-1 alpha is differentially processed, and different cells secrete the two isoforms (5, 7). Mature feline SDF-1 beta is 96%, 97% and 100% aa identical to rat, mouse and human SDF-1 beta , respectively. Human (and by inference, feline) SDF-1 is active on mouse cells. SDF-1 alpha and beta are reported to be a monomers at neutral pH and physiologic ionic strength (4). SDF-1 alpha is also reported to form dimers in the presence of heparan sulfate (8). On the cell surface, this may well facilitate SDF-1 interaction with its two receptors, CXCR4 and syndecan-4 (9). Heparan sulfate is known to protect SDF-1 from proteolysis, and CXCR4 exists constitutively as a dimer (9 - 11). Among its many functions, CXCL12 is known to influence lymphopoiesis, regulate patterning and cell number of neural progenitors, and promote angiogenesis (12, 13). It also enhances the survival of myeloid progenitor cells.

1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
2. Rollins, B.J. (1997) Blood 90:909.
3. Nishimura, Y. et al. (1998) Eur. J. Immunogenet. 25:303.
4. Crump, M.P. et al. (1997) EMBO J. 16:6996.
5. Sierra, M.D. L. et al. (2004) Blood 103:2452.
6. Davis, D.A. et al. (2005) Blood 105:4561.
7. Stumm, R.K. et al. (2002) J. Neurosci. 22:5865.
8. Veldkamp, C.T. et al. (2005) Protein Sci. 14:1071.
9. Charnaux, N. et al. (2005) FEBS J. 272:1937.
10. Percherancier, Y. et al. (2005) J. Biol. Chem. 280:9895.
11. Babcock, G.J. et al. (2003) J. Biol. Chem. 278:3378.
12. Klein, R.S. et al. (2004) Trends Immunol. 25:306.
13. Salcedo, R. and J.J. Oppenheim (2003) Microcirculation 10:359.
14. Broxmeyer, H.E. et al. (2003) J. Leukoc. Biol. 73:630.

Publications for CXCL12/SDF-1 beta (2716-SD/CF)(2)

Publications using 2716-SD/CF

• Hsiao J, Ng B, Smits M, Wang J, Jasavala R, Martinez H, Lee J, Alston J, Misonou H, Trimmer J, Wright M Androgen receptor and chemokine receptors 4 and 7 form a signaling axis to regulate CXCL12-dependent cellular motility. BMC Cancer, 2015-03-31;15(0):204. 2015-03-31 [PMID: 25884570] (Bioassay, Human)
• Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain. Stem Cells, 2007-10-11;25(12):3261-70. 2007-10-11 [PMID: 17932418] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque))

Bioinformatics

• Gene Symbol: CXCL12
• Uniprot:
  • BAA28602()