Recombinant Human Fc gamma RIIA/CD32a (R167) Protein

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1 μg/lane of Recombinant Human Fc gamma RIIA/CD32a (R167) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 32 kDa.
Recombinant Human Fc gamma RIIA/CD32a (R167) (Catalog # 1330-CD) binds human IgG with an estimated KD <80 nM.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity

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Catalog# & Formulation Size Price

Recombinant Human Fc gamma RIIA/CD32a (R167) Protein Summary

Details of Functionality
Measured by its ability to bind human IgG with an estimated Kd <80 nM.
Source
Mouse myeloma cell line, NS0-derived human Fc gamma RIIA/CD32a protein
Ala36-Ile218, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ala36
Protein/Peptide Type
Recombinant Proteins
Gene
FCGR2A
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
32 kDa, reducing conditions
Publications
Read Publications using
1330-CD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fc gamma RIIA/CD32a (R167) Protein

  • CD32 antigen
  • CD32a
  • CD32MGC23887
  • CDw32fc-gamma-RIIa
  • Fc fragment of IgG, low affinity IIa, receptor (CD32)
  • Fc fragment of IgG, low affinity IIa, receptor for (CD32)
  • Fc gamma RIIA
  • FCG2
  • Fc-gamma RII-a
  • Fc-gamma-RIIa
  • FcGR
  • FCGR2
  • FCGR2A
  • FCGR2A1
  • FcgRIIA
  • FCRIIA
  • fcRII-a
  • IGFR2MGC30032
  • IgG Fc receptor II-a
  • Immunoglobulin G Fc receptor II
  • low affinity immunoglobulin gamma Fc region receptor II-a

Background

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1-3). The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC).

Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified (1-3). The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides IC, Fc gamma RII A also binds C-reactive protein (CRP) (4, 5). Two allelic variants (R167 and H167) of Fc gamma RIIA that differ in their ability to ligate human IgG2 or CRP exist. The H167 allele has been found to have a protective effect against lupus nephritis.

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Chi, M. et al. (2002) J. Immunol. 168:1413.
  5. Zuniga, R. et al. (2003) Arthritis Rheum. 48:460.

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1330-CD
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Applications: Binding Activity

Publications for Fc gamma RIIA/CD32a (1330-CD)(11)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 4 applications: Bioassay, ELISA (Capture), Surface Plasmon Resonance, Surface Plasmon Resonance (SPR.


Filter By Application
Bioassay
(5)
ELISA (Capture)
(2)
Surface Plasmon Resonance
(2)
Surface Plasmon Resonance (SPR
(2)
All Applications
Filter By Species
Human
(8)
Mouse
(1)
N/A
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 1330-CD Applications Species
PA Blundell, D Lu, M Wilkinson, A Dell, S Haslam, RJ Pleass Insertion of N-Terminal Hinge Glycosylation Enhances Interactions of the Fc Region of Human IgG1 Monomers with Glycan-Dependent Receptors and Blocks Hemagglutination by the Influenza Virus J. Immunol., 2019;0(0):. 2019 [PMID: 30683699] (ELISA (Capture), Human) ELISA (Capture) Human
T Oshima, H Miyashita, Y Ishimura, Y Ito, Y Tanaka, A Hori, T Kokubo, T Kurokawa Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey PLoS ONE, 2018;13(5):e0196422. 2018 [PMID: 29723247] (Bioassay) Bioassay
M Gabriel, DI Adomeh, J Ehimuan, J Oyakhilome, EO Omomoh, Y Ighodalo, T Olokor, K Bonney, M Pahlmann, P Emmerich, M Lelke, L Brunotte, S Ölschläger, C Thomé-Bold, B Becker-Zia, C Busch, I Odia, E Ogbaini-Em, PO Okokhere, SA Okogbenin, GO Akpede, H Schmitz, DA Asogun, S Günther Development and evaluation of antibody-capture immunoassays for detection of Lassa virus nucleoprotein-specific immunoglobulin M and G PLoS Negl Trop Dis, 2018;12(3):e0006361. 2018 [PMID: 29596412] (Bioassay, Human) Bioassay Human
F Arce Varga, AJS Furness, K Litchfield, K Joshi, R Rosenthal, E Ghorani, I Solomon, MH Lesko, N Ruef, C Roddie, JY Henry, L Spain, A Ben Aissa, A Georgiou, YNS Wong, M Smith, D Strauss, A Hayes, D Nicol, T O'Brien, L Mårtensson, A Ljungars, I Teige, B Frendéus,,,, M Pule, T Marafioti, M Gore, J Larkin, S Turajlic, C Swanton, KS Peggs, SA Quezada Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies Cancer Cell, 2018;0(0):. 2018 [PMID: 29576375] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
N Seo, A Polozova, M Zhang, Z Yates, S Cao, H Li, S Kuhns, G Maher, HJ McBride, J Liu Analytical and functional similarity of Amgen biosimilar ABP 215 to bevacizumab MAbs, 2018;0(0):1-58. 2018 [PMID: 29553864] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
S Järnum, A Runström, R Bockermann, L Winstedt, M Crispin, C Kjellman Enzymatic inactivation of endogenous IgG by IdeS enhances�therapeutic antibody efficacy Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 28533435] (Bioassay, Human) Bioassay Human
Zhang X, Olsen H, Chen S, So E, Zhou H, Burch E, Merigeon E, Block D, Strome S Anti-CD20 Antibody with Multimerized Fc Domains: A Novel Strategy To Deplete B Cells and Augment Treatment of Autoimmune Disease. J Immunol, 2016;196(3):1165-76. 2016 [PMID: 26695368] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
Meuris L, Santens F, Elson G, Festjens N, Boone M, Dos Santos A, Devos S, Rousseau F, Plets E, Houthuys E, Malinge P, Magistrelli G, Cons L, Chatel L, Devreese B, Callewaert N GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins. Nat Biotechnol, 2014;32(5):485-9. 2014 [PMID: 24752077] (Bioassay, Human) Bioassay Human
Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases. J Immunol, 2014;192(9):4083-92. 2014 [PMID: 24670803] (Bioassay, Human) Bioassay Human
Bergin DA, Reeves EP, Meleady P, Henry M, McElvaney OJ, Carroll TP, Condron C, Chotirmall SH, Clynes M, O'Neill SJ, McElvaney NG Alpha-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8. J. Clin. Invest., 2010;120(12):4236-50. 2010 [PMID: 21060150] (ELISA (Capture), Human) ELISA (Capture) Human
Show All 11 Publications.

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FAQs for Fc gamma RIIA/CD32a (1330-CD). (Showing 1 - 1 of 1 FAQs).

  1. Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?
    • For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.

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Bioinformatics

Gene Symbol FCGR2A
Uniprot