Recombinant Human EGFR Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human EGFR Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind recombinant human EGF in a functional ELISA with an estimated Kd <8 nM.
Source
Mouse myeloma cell line, NS0-derived human EGFR protein
Human EGFR
(Met1-Ser645)
Accession # CAA25240
IEGRMD Human IgG1-Fc
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu25
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
EGFR
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
95.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
125-145 kDa, reducing conditions
Publications
Read Publications using
344-ER in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EGFR Fc Chimera Protein, CF

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC 2.7.10.1
  • EGF R
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ErbB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1

Background

The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular ligand binding domain containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The human EGFR cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 621 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 542 aa cytoplasmic domain (3, 4). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (5‑7). Within the ECD, human EGFR shares 88% aa sequence identity with mouse and rat EGFR. It shares 43%-44% aa sequence identity with the ECD of human ErbB2, ErbB3, and ErbB4. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (8‑12). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (13, 14). EGFR is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs (15).

  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Lin, C. et al. (1984) Science 224:843.
  4. Ullrich, A. et al. (1984) Nature 309:418.
  5. Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050.
  6. Reiter J.L. et al. (2001) Genomics 71:1.
  7. Xu, Y.H. et al. (1984) Nature 309:806.
  8. Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
  9. Yarden, Y. et al. (1987) Biochemistry 26:1434.
  10. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  11. Lemmon, M.A. et al. (1997) EMBO J. 16:281.
  12. Cohen, S. et al. (1982) J. Biol. Chem. 257:1523.
  13. Sibilia, M. and E.F. Wagner (1995) Science 269:234.
  14. Miettinen, P.J. et al. (1995) Nature 376:337.
  15. Roskoski Jr., R. (2004) Biochem. Biophys. Res. Commun. 319:1.

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344-ER
Species: Hu
Applications: Binding Activity

Publications for EGFR (344-ER)(12)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 8 applications: Bioassay, CAR-T, Direct ELISA, Dot Blot, ELISA (Capture), ELISA Developmet, Flow Cytometry, Western Blot.


Filter By Application
Bioassay
(4)
CAR-T
(1)
Direct ELISA
(1)
Dot Blot
(1)
ELISA (Capture)
(1)
ELISA Developmet
(1)
Flow Cytometry
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(8)
N/A
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 344-ER Applications Species
S Ahn, J Li, C Sun, K Gao, K Hirabayash, H Li, B Savoldo, R Liu, G Dotti Cancer immunotherapy with T cells carrying bispecific receptors that mimic antibodies Cancer Immunol Res, 2019;0(0):. 2019 [PMID: 30842091] (CAR-T, Human) CAR-T Human
S Pankratova, J Klingelhof, O Dmytriyeva, S Owczarek, A Renziehaus, N Syed, AE Porter, DT Dexter, D Kiryushko The S100A4 Protein Signals through the ErbB4 Receptor to Promote Neuronal Survival Theranostics, 2018;8(14):3977-3990. 2018 [PMID: 30083275] (Human) Human
SAA Kooijmans, JJJM Gitz-Franc, RM Schiffeler, P Vader Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach Nanoscale, 2018;0(0):. 2018 [PMID: 29334397] (ELISA (Capture), Human) ELISA (Capture) Human
M Yun, DY Kim, JJ Lee, HS Kim, HS Kim, A Pyo, Y Ryu, TY Kim, JH Zheng, SW Yoo, H Hyun, G Oh, J Jeong, M Moon, JH Min, SY Kwon, JY Kim, E Chung, Y Hong, W Lee, HS Kim, JJ Min A High-Affinity Repebody for Molecular Imaging of EGFR-Expressing Malignant Tumors Theranostics, 2017;7(10):2620-2633. 2017 [PMID: 28819451] (ELISA Developmet, N/A) ELISA Developmet N/A
V Wycisk, K Achazi, O Hirsch, C Kuehne, J Dernedde, R Haag, K Licha Heterobifunctional Dyes: Highly Fluorescent Linkers Based on Cyanine Dyes ChemistryOpen, 2017;6(3):437-446. 2017 [PMID: 28638777] (Bioassay) Bioassay
C Stegmann, D Hochdorfer, D Lieber, N Subramania, D Stöhr, K Laib Sampa, C Sinzger A derivative of platelet-derived growth factor receptor alpha binds to the trimer of human cytomegalovirus and inhibits entry into fibroblasts and endothelial cells PLoS Pathog., 2017;13(4):e1006273. 2017 [PMID: 28403220] (Bioassay, Human) Bioassay Human
X Han, PD Bryson, Y Zhao, GE Cinay, S Li, Y Guo, N Siriwon, P Wang Masked Chimeric Antigen Receptor for Tumor-Specific Activation Mol. Ther, 2017;25(1):274-284. 2017 [PMID: 28129121] (Flow Cytometry, Human) Flow Cytometry Human
A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents J. Mol. Biol., 2016;0(0):. 2016 [PMID: 27887869] (Direct ELISA, Human) Direct ELISA Human
Sachdev S Sidhu A Highly Diverse and Functional Na�ve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents J. Mol. Biol., 2016;0(0):. 2016 [PMID: 27887869] (Western Blot) Western Blot
A Novel Bispecific Antibody Targeting EGFR and cMet that is Effective Against EGFR Inhibitor- Resistant Lung Tumors Cancer Res, 2016;0(0):. 2016 [PMID: 27216193] (Bioassay, Human) Bioassay Human
Show All 12 Publications.

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Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Niemann Pick-C1 and cholesterol dynamics
Niemann-Pick type C1 (NPC1) mediates low-density cholesterol transport from late endosomes and lysosomes to other areas of the cell via receptor mediation endocytosis.  Although cholesterol moves freely inside the cell, it cannot independently expo...  Read full blog post.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

It's a Wiz: Merlin Antibodies Advance Hepatic Tumor Research
The NF2 gene, also known as “Merlin”, was discovered through studies into Neurofibromatosis Type II, a rare genetic disease which causes formation of non-malignant, but life-limiting, brain tumors. NF2 encodes a cytoskeletal protein involved in extrac...  Read full blog post.

The Magic of Merlin: Antibodies Point to New Role in Liver Cancer
The Merlin protein belongs to the ezrin/radixin/moesin (ERM) family of tumour suppressor proteins. Encoded by the rather less imaginatively named Neurofibromin 2 (NF2) gene, it is thought to play a role in extracellular signal transduction, linking th...  Read full blog post.

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Bioinformatics

Gene Symbol EGFR
Uniprot