Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

Product Discontinued

Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 10⁴ cells/well are added to Recombinant Human DC‑SIGN/CD209 Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately 50-80% of added cells will adhere after 1 hour at room temperature.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human DC-SIGN/CD209 protein
  

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Human DC-SIGN (Lys62-Ala404) Accession # Q9NNX6
  N-terminus			C-terminus

• Accession #: Q9NNX6
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CD209
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 65.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

• CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
• CD209 molecule
• CD209
• CDSIGNHIV gpl20-binding protein
• CLEC4L
• CLEC4LC-type lectin domain family 4 member L
• DCSIGN
• DC-SIGN
• DC-SIGN1
• DC-SIGN1C-type lectin domain family 4, member L
• DC-SIGNMGC129965
• Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Background

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca²⁺-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).

1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Publications for DC-SIGN/CD209 (161-DC)(17)

Publications using 161-DC

• Lei Guo, Yan Liang, Heng Li, Huiwen Zheng, Zening Yang, Yanli Chen et al. Epigenetic glycosylation of SARS-CoV-2 impact viral infection through DC&L-SIGN receptors iScience 2021-12-17 [PMID: 34786539] (ELISA Capture, N/A)
• C Gao, K Stavenhage, B Eckmair, TR McKitrick, AY Mehta, Y Matsumoto, AM McQuillan, MS Hanes, D Eris, KJ Baker, N Jia, M Wei, J Heimburg-M, B Ernst, RD Cummings Differential recognition of oligomannose isomers by glycan-binding proteins involved in innate and adaptive immunity Science Advances, 2021-06-09;7(24):. 2021-06-09 [PMID: 34108208] (Bioassay, Human)
• J Cvetkovic, N Ilic, A Gruden-Mov, S Tomic, N Mitic, E Pinelli, L Sofronic-M DC-SIGN signalling induced by Trichinella spiralis products contributes to the tolerogenic signatures of human dendritic cells Sci Rep, 2020-11-20;10(1):20283. 2020-11-20 [PMID: 33219293] (Bioassay, Rat)
• EE Mouser, G Pollakis, HH Smits, J Thomas, M Yazdanbakh, EC de Jong, WA Paxton Schistosoma mansoni soluble egg antigen (SEA) and recombinant Omega-1 modulate induced CD4+ T-lymphocyte responses and HIV-1 infection in vitro PLoS Pathog., 2019-09-05;15(9):e1007924. 2019-09-05 [PMID: 31487324] (Bioassay, ELISA Detection, Parasite - Schistosoma mansoni, Plant)
• X Zhang, J Owens, HS Olsen, E So, E Burch, MC McCroskey, X Li, GL Weber, D Bennett, D Rybin, H Zhou, H Hao, EY Mérigeon, DS Block, G LaRosa, SE Strome A recombinant human IgG1 Fc multimer designed to mimic the active fraction of IVIG in autoimmunity JCI Insight, 2019-01-24;4(2):. 2019-01-24 [PMID: 30674715] (Surface Plasmon Resonance, Human)
• LL Eggink, KF Roby, R Cote, J Kenneth Ho An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides J Immunother Cancer, 2018-04-17;6(1):28. 2018-04-17 [PMID: 29665849] (Binding Assay, Human)
• Z Zou, J Misasi, N Sullivan, PD Sun Overexpression of Ebola virus envelope GP1 protein Protein Expr. Purif., 2017-04-27;0(0):. 2017-04-27 [PMID: 28458053] (Enzyme Assay, Virus - Ebola)
• Mouser E, Pollakis G, Yazdanbakhsh M, Harnett W, de Jong E, Paxton W Brugia malayi Antigen (BmA) Inhibits HIV-1 Trans-Infection but Neither BmA nor ES-62 Alter HIV-1 Infectivity of DC Induced CD4+ Th-Cells. PLoS ONE, 2016-01-25;11(1):e0146527. 2016-01-25 [PMID: 26808476] (ELISA Developmet, Nematode - Brugia malayi)
• Peters M, Guidato P, Peters K, Megger D, Sitek B, Classen B, Heise E, Bufe A Allergy-Protective Arabinogalactan Modulates Human Dendritic Cells via C-Type Lectins and Inhibition of NF-kappaB. J Immunol, 2016-01-08;196(4):1626-35. 2016-01-08 [PMID: 26746190] (Binding Assay)
• Stax M, Mouser E, van Montfort T, Sanders R, de Vries H, Dekker H, Herrera C, Speijer D, Pollakis G, Paxton W Colorectal mucus binds DC-SIGN and inhibits HIV-1 trans-infection of CD4+ T-lymphocytes. PLoS ONE, 2015-03-20;10(3):e0122020. 2015-03-20 [PMID: 25793526] (Bioassay, Human)
• Jiang , Yanmei, Zhang , Changfu, Chen , Kai, Chen , Zhe, Sun , Zhigang, Zhang , Zhuqing, Ding , Dongbing, Ren , Shuangyi, Zuo , Yunfei The clinical significance of DC-SIGN and DC-SIGNR, which are novel markers expressed in human colon cancer. PLoS ONE, 2014-12-12;9(12):e114748. 2014-12-12 [PMID: 25504222] (ELISA (Standard), Human)
• Emara M, Royer PJ, Mahdavi J Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite. J. Biol. Chem., 2011-12-28;287(8):5756-63. 2011-12-28 [PMID: 22205703] (ELISA (detection), Canine, Mite)
• Johnson TR, McLellan JS, Graham BS Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. J. Virol., 2011-11-16;86(3):1339-47. 2011-11-16 [PMID: 22090124] (Surface Plasmon Resonance, Virus)
• Sabatte J, Faigle W, Ceballos A, Morelle W, Rodrígues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S Semen clusterin is a novel DC-SIGN ligand. J. Immunol., 2011-10-17;187(10):5299-309. 2011-10-17 [PMID: 22013110] (Surface Plasmon Resonance, Western Blot (Detection), Human)
• Gustafsson A, Sjoblom M, Strindelius L, Johansson T, Fleckenstein T, Chatzissavidou N, Lindberg L, Angstrom J, Rova U, Holgersson J Pichia pastoris-produced mucin-type fusion proteins with multivalent O-glycan substitution as targeting molecules for mannose-specific receptors of the immune system. Glycobiology, 2011-04-07;21(8):1071-86. 2011-04-07 [PMID: 21474492] (Surface Plasmon Resonance, Human)
• Stax MJ, van Montfort T, Sprenger RR, Melchers M, Sanders RW, van Leeuwen E, Repping S, Pollakis G, Speijer D, Paxton WA Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4(+) T-lymphocytes. Virology, 2009-09-01;391(2):203-11. 2009-09-01 [PMID: 19682628] (Western Blot (Detection), Human)
• Nonaka M, Ma BY, Murai R, Nakamura N, Baba M, Kawasaki N, Hodohara K, Asano S, Kawasaki T Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells. J. Immunol., 2008-03-01;180(5):3347-56. 2008-03-01 [PMID: 18292560] (Binding Assay, Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: CD209
• Uniprot:
  • Q9NNX6()