Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF Summary

Additional Information
A New and Improved rh DC-SIGN is Now Available! The new protein has enhanced purity and a HA-tag!
Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 104 cells/well are added to Recombinant Human DC‑SIGN/CD209 Fc Chimera coated plates (5 µg/mL with 100 µL/well), approximately 50-80% of added cells will adhere after 1 hour at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human DC-SIGN/CD209 protein
MD Human IgG1
(Pro100-Lys330)
IEGR Human DC-SIGN
(Lys62-Ala404)
Accession # Q9NNX6
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CD209
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
65.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
65-75 kDa, reducing conditions
Publications
Read Publications using
161-DC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DC-SIGN/CD209 Fc Chimera Protein, CF

  • CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin
  • CD209 molecule
  • CD209
  • CDSIGNHIV gpl20-binding protein
  • CLEC4L
  • CLEC4LC-type lectin domain family 4 member L
  • DCSIGN
  • DC-SIGN
  • DC-SIGN1
  • DC-SIGN1C-type lectin domain family 4, member L
  • DC-SIGNMGC129965
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 1

Background

Human Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209 is a member of the C-type lectin family (1). The canonical DC-SIGN/CD209 isoform is a 46 kDa, 404 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms (3). DC-SIGN/CD209 is not well conserved between mouse and human, with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (5, 6).
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

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Publications for DC-SIGN/CD209 (161-DC)(14)

We have publications tested in 6 confirmed species: Human, Canine, Nematode - Brugia malayi, Parasite - Schistosoma mansoni, Virus, Virus - Ebola.

We have publications tested in 8 applications: Binding Assay, Bioassay, ELISA (Detection), ELISA (Standard), ELISA Developmet, Enzyme Assay, Surface Plasmon Resonance, Western Blot (Detection).


Filter By Application
Binding Assay
(1)
Bioassay
(2)
ELISA (Detection)
(1)
ELISA (Standard)
(1)
ELISA Developmet
(1)
Enzyme Assay
(1)
Surface Plasmon Resonance
(4)
Western Blot (Detection)
(1)
All Applications
Filter By Species
Human
(7)
Canine
(1)
Nematode - Brugia malayi
(1)
Parasite - Schistosoma mansoni
(1)
Virus
(1)
Virus - Ebola
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 161-DC Applications Species
EE Mouser, G Pollakis, HH Smits, J Thomas, M Yazdanbakh, EC de Jong, WA Paxton Schistosoma mansoni soluble egg antigen (SEA) and recombinant Omega-1 modulate induced CD4+ T-lymphocyte responses and HIV-1 infection in vitro PLoS Pathog., 2019;15(9):e1007924. 2019 [PMID: 31487324] (Bioassay, Parasite - Schistosoma mansoni) Bioassay Parasite - Schistosoma mansoni
X Zhang, J Owens, HS Olsen, E So, E Burch, MC McCroskey, X Li, GL Weber, D Bennett, D Rybin, H Zhou, H Hao, EY Mérigeon, DS Block, G LaRosa, SE Strome A recombinant human IgG1 Fc multimer designed to mimic the active fraction of IVIG in autoimmunity JCI Insight, 2019;4(2):. 2019 [PMID: 30674715] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
LL Eggink, KF Roby, R Cote, J Kenneth Ho An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides J Immunother Cancer, 2018;6(1):28. 2018 [PMID: 29665849] (Binding Assay, Human) Binding Assay Human
Z Zou, J Misasi, N Sullivan, PD Sun Overexpression of Ebola virus envelope GP1 protein Protein Expr. Purif., 2017;0(0):. 2017 [PMID: 28458053] (Enzyme Assay, Virus - Ebola) Enzyme Assay Virus - Ebola
Mouser E, Pollakis G, Yazdanbakhsh M, Harnett W, de Jong E, Paxton W Brugia malayi Antigen (BmA) Inhibits HIV-1 Trans-Infection but Neither BmA nor ES-62 Alter HIV-1 Infectivity of DC Induced CD4+ Th-Cells. PLoS ONE, 2016;11(1):e0146527. 2016 [PMID: 26808476] (ELISA Developmet, Nematode - Brugia malayi) ELISA Developmet Nematode - Brugia malayi
Peters M, Guidato P, Peters K, Megger D, Sitek B, Classen B, Heise E, Bufe A Allergy-Protective Arabinogalactan Modulates Human Dendritic Cells via C-Type Lectins and Inhibition of NF-kappaB. J Immunol, 2015;196(4):1626-35. 2015 [PMID: 26746190]
Stax M, Mouser E, van Montfort T, Sanders R, de Vries H, Dekker H, Herrera C, Speijer D, Pollakis G, Paxton W Colorectal mucus binds DC-SIGN and inhibits HIV-1 trans-infection of CD4+ T-lymphocytes. PLoS ONE, 2015;10(3):e0122020. 2015 [PMID: 25793526] (Bioassay, Human) Bioassay Human
Jiang, Yanmei, Zhang, Changfu, Chen, Kai, Chen, Zhe, Sun, Zhigang, Zhang, Zhuqing, Ding, Dongbing, Ren, Shuangyi, Zuo, Yunfei The clinical significance of DC-SIGN and DC-SIGNR, which are novel markers expressed in human colon cancer. PLoS ONE, 2014;9(12):e114748. 2014 [PMID: 25504222] (ELISA (Standard), Human) ELISA (Standard) Human
Emara M, Royer PJ, Mahdavi J Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite. J. Biol. Chem., 2012;287(8):5756-63. 2012 [PMID: 22205703] (ELISA (Detection), Canine) ELISA (Detection) Canine
Johnson TR, McLellan JS, Graham BS Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2. J. Virol., 2012;86(3):1339-47. 2012 [PMID: 22090124] (Surface Plasmon Resonance, Virus) Surface Plasmon Resonance Virus
Show All 14 Publications.

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Bioinformatics

Gene Symbol CD209
Uniprot