Recombinant Human CXCL7/NAP-2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CXCL7/NAP-2 Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED₅₀ for this effect is 0.200-1.60 ng/mL.
• Source: E. coli-derived human CXCL7/NAP-2 protein
  Ala59-Asp128
• Accession #: P02775
• N-terminal Sequence: Ala59
• Protein/Peptide Type: Recombinant Proteins
• Gene: PPBP
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 7.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXCL7/NAP-2 Protein, CF

• Beta-TG
• connective tissue-activating peptide III
• CTAP III
• CTAP3CTAP-III
• CTAPIII
• CXC chemokine ligand 7
• C-X-C motif chemokine 7
• CXCL7
• CXCL7b-TG1
• LA-PF4
• LDGFTC1
• Leukocyte-derived growth factor
• low-affinity platelet factor IV
• Macrophage-derived growth factor
• MDGFTC2
• NAP2
• NAP-2
• NAP-2-L1
• neutrophil-activating peptide 2
• neutrophil-activating peptide-2
• PBPTHBGB
• platelet basic protein
• PPBP
• pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
• SCAR10
• SCYB7beta-thromboglobulin
• small inducible cytokine B7
• small inducible cytokine subfamily B, member 7
• Small-inducible cytokine B7
• TGB
• TGB1B-TG1
• THBGB1
• thrombocidin 1
• thrombocidin 2
• thromboglobulin, beta-1

Background

Neutrophil Activating Peptide 2 (NAP-2), Connective Tissue Activating Protein III (CTAP-III) and beta -thrombogulin ( beta -TG), are proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR2 and to chemoattract and activate neutrophils. Although CTAP-III, beta -TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. It has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

1. Schall, T. (1994) The Cytokine Handbook, 2nd edition, A. Thomson, ed. Academic Press, New York, p. 419.
2. Malkowski, M.G. et al. (1997) J. Mol. Biol. 266:367.

Publications for CXCL7/NAP-2 (393-NP/CF)(7)

Publications using 393-NP/CF

• K Egan, JP van Geffen, H Ma, B Kevane, A Lennon, S Allen, E Neary, M Parsons, P Maguire, K Wynne, R O' Kennedy, JW Heemskerk, FN Áinle Effect of platelet-derived ?-thromboglobulins on coagulation Thromb. Res., 2017-03-30;154(0):7-15. 2017-03-30 [PMID: 28384443] (Bioassay, Human)
• Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO J., 2012-07-31;31(17):3607-19. 2012-07-31 [PMID: 22850671] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Kirouac DC, Ito C, Csaszar E, Roch A, Yu M, Sykes EA, Bader GD, Zandstra PW Dynamic interaction networks in a hierarchically organized tissue. Mol. Syst. Biol., 2010-10-05;6(0):417. 2010-10-05 [PMID: 20924352] (Bioassay, Human)
• Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8. J. Biol. Chem., 2006-12-29;282(16):11658-66. 2006-12-29 [PMID: 17197447] (Binding Assay, Mouse)
• Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. J. Am. Coll. Cardiol., 2006-09-27;48(8):1591-9. 2006-09-27 [PMID: 17045893] (Bioassay, Human)
• Traves SL, Smith SJ, Barnes PJ, Donnelly LE Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2. J. Leukoc. Biol., 2004-05-20;76(2):441-50. 2004-05-20 [PMID: 15155777] (Bioassay, Human)

Bioinformatics

• Gene Symbol: PPBP
• Uniprot:
  • P02775()