Recombinant Human CXCL7/NAP-2 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA, BA2

Order Details

Recombinant Human CXCL7/NAP-2 Protein Summary

Details of Functionality
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 0.1‑0.3 μg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.1‑0.5 ng/mL.
Source
E. coli-derived
Ala59-Asp128
Accession #
N-terminal Sequence
Ala59
Protein/Peptide Type
Recombinant Proteins
Gene
PPBP
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
393-NP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXCL7/NAP-2 Protein

  • Beta-TG
  • connective tissue-activating peptide III
  • CTAP III
  • CTAP3CTAP-III
  • CTAPIII
  • CXC chemokine ligand 7
  • C-X-C motif chemokine 7
  • CXCL7
  • CXCL7b-TG1
  • LA-PF4
  • LDGFTC1
  • Leukocyte-derived growth factor
  • low-affinity platelet factor IV
  • Macrophage-derived growth factor
  • MDGFTC2
  • NAP2
  • NAP-2
  • NAP-2-L1
  • neutrophil-activating peptide 2
  • neutrophil-activating peptide-2
  • PBPTHBGB
  • platelet basic protein
  • PPBP
  • pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
  • SCAR10
  • SCYB7beta-thromboglobulin
  • small inducible cytokine B7
  • small inducible cytokine subfamily B, member 7
  • Small-inducible cytokine B7
  • TGB
  • TGB1B-TG1
  • THBGB1
  • thrombocidin 1
  • thrombocidin 2
  • thromboglobulin, beta-1

Background

Neutrophil Activating Peptide 2 (NAP-2), Connective Tissue Activating Protein III (CTAP-III) and beta -thrombogulin ( beta -TG), are proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR2 and to chemoattract and activate neutrophils. Although CTAP-III, beta -TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. It has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

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393-NP
Species: Hu
Applications: BA, BA2

Publications for (6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Binding Assay, Bioassay.


Filter By Application
Binding Assay
(1)
Bioassay
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 393-NP Applications Species
Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis. EMBO J., 2012;31(17):3607-19. 2012 [PMID: 22850671] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Kirouac DC, Ito C, Csaszar E, Roch A, Yu M, Sykes EA, Bader GD, Zandstra PW Dynamic interaction networks in a hierarchically organized tissue. Mol. Syst. Biol., 2010;6(0):417. 2010 [PMID: 20924352] (Bioassay, Human) Bioassay Human
Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8. J. Biol. Chem., 2007;282(16):11658-66. 2007 [PMID: 17197447] (Binding Assay, Mouse) Binding Assay Mouse
Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation. J. Am. Coll. Cardiol., 2006;48(8):1591-9. 2006 [PMID: 17045893] (Bioassay, Human) Bioassay Human
Traves SL, Smith SJ, Barnes PJ, Donnelly LE Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2. J. Leukoc. Biol., 2004;76(2):441-50. 2004 [PMID: 15155777] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol PPBP
Entrez
Uniprot