Recombinant Human CCL3/MIP-1 alpha Protein

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Recombinant Human CCL3/MIP-1 alpha Isoform LD78a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro-B cells transfected with human CCR5. The ED50 for this effect is 3-10 ng/mL.
1 μg/lane of Recombinant Human CCL3/MIP-1 alpha Isoform LD78a was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human CCL3/MIP-1 alpha Protein Summary

Details of Functionality
Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 2‑10 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 3‑10 ng/mL.
Source
E. coli-derived human CCL3/MIP-1 alpha protein
Ala27-Ala92
Accession #
N-terminal Sequence
Ala27
Protein/Peptide Type
Recombinant Proteins
Gene
CCL3
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
270-LD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL3/MIP-1 alpha Protein

  • C-C motif chemokine 3
  • MIP1-(a)
  • AI323804
  • CCL3
  • chemokine (C-C motif) ligand 3
  • G0S19-1
  • LD78a
  • LD78alpha
  • MIP1 alpha
  • MIP-1 alpha
  • Mip1a
  • MIP-1alpha
  • MIP1-alpha
  • Scya3

Background

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature human CCL3 shares 70% - 74% amino acid sequence identity with mouse, rat, and cotton rat CCL3 (2). CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (3, 4). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (5, 6). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (6). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (7). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 8 - 10). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (3). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (11 - 14), although it also can suppress the replication of HIV (15). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1-mediated noxious stimulation (16).
  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  2. Obaru, K. et al. (1986) J. Biochem. 99:885.
  3. Graham, G.J. et al. (1990) Nature 344:442.
  4. Wagner, L. et al. (1998) Nature 391:908.
  5. Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
  6. Ren, M. et al. (2010) EMBO J. 29:3952.
  7. Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
  8. Taub, D.D. et al. (1993) Science 260:355.
  9. Bernardini, G. et al. (2008) Blood 111:3626.
  10. Lee, S.C. et al. (2000) J. Immunol. 164:3392.
  11. Wu, Y. et al. (2008) J. Immunol. 181:6384.
  12. Cook, D.N. et al. (1995) Science 269:1583.
  13. Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
  14. Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
  15. Cocchi, F. et al. (1995) Science 270:1811.
  16. Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.

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270-LD
Species: Hu
Applications: Bioactivity

Publications for CCL3/MIP-1 alpha (270-LD)(33)

We have publications tested in 5 confirmed species: Human, Rat, N/A, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA (Standard), Hemofiltration Procedure.


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Binding Assay
(2)
Bioassay
(20)
ELISA (Standard)
(9)
Hemofiltration Procedure
(1)
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Filter By Species
Human
(19)
Rat
(1)
N/A
(9)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
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(1)
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Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using 270-LD Applications Species
H Nakano, Y Kirino, M Takeno, K Higashitan, H Nagai, R Yoshimi, Y Yamaguchi, I Kato, I Aoki, H Nakajima GWAS-identified CCR1 and IL10 loci contribute to M1 macrophage-predominant inflammation in Beh�et&#039;s disease Arthritis Res. Ther., 2018;20(1):124. 2018 [PMID: 29895319] (Bioassay, Human) Bioassay Human
MH van Atteku, S Terpstra, E Slinger, M von Linder, PD Moerland, A Jongejan, AP Kater, E Eldering Macrophages confer survival signals via CCR1-dependent translational MCL-1 induction in chronic lymphocytic leukemia Oncogene, 2017;0(0):. 2017 [PMID: 28192408] (Bioassay, Human) Bioassay Human
Benjamin Malard Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study Artif Organs, 2016;0(0):. 2016 [PMID: 27911005] (Hemofiltration Procedure, Human) Hemofiltration Procedure Human
Stefanie Karlshøj Molecular Mechanism of Action for Allosteric Modulators and Agonists in CC-chemokine Receptor 5 (CCR5) J. Biol. Chem, 2016;291(52):26860-26874. 2016 [PMID: 27834679] (Bioassay) Bioassay
Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies Mucosal Immunol, 2016;9(6):1584-1595. 2016 [PMID: 26883728] (Bioassay, Human) Bioassay Human
Gouwy M, De Buck M, Portner N, Opdenakker G, Proost P, Struyf S, Van Damme J Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines. Eur J Immunol, 2015;45(1):101-12. 2015 [PMID: 25345597] (Bioassay, Human) Bioassay Human
Ravi A, Khurana S, Lemon J, Plumb J, Booth G, Healy L, Catley M, Vestbo J, Singh D Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum. Respir Res, 2014;15(0):103. 2014 [PMID: 25183374] (Bioassay, Human) Bioassay Human
Buttari B, Profumo E, Domenici G, Tagliani A, Ippoliti F, Bonini S, Businaro R, Elenkov I, Rigano R Neuropeptide Y induces potent migration of human immature dendritic cells and promotes a Th2 polarization. FASEB J, 0;28(7):3038-49. 0 [PMID: 24699455] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Badr G, Lefevre EA, Mohany M Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis. PLoS ONE, 2011;6(9):e23741. 2011 [PMID: 21912642] (Bioassay, Human) Bioassay Human
Show All 33 Publications.

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Bioinformatics

Gene Symbol CCL3
Uniprot