Recombinant Human BMP-7 Protein, CF


Recombinant human BMP-7 (354-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.1-0.6 µg/mL.
Adult stem cell-derived liver organoids were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and liver organoid initiation medium, which includes Recombinant Human BMP-7 (Catalog # more

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-7 Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.1-0.6 µg/mL.
Chinese Hamster Ovary cell line, CHO-derived human BMP-7 protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
15.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
18-20 kDa, reducing conditions
Read Publications using
354-BP/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile 4 mM HCl.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-7 Protein, CF

  • BMP7
  • BMP-7
  • bone morphogenetic protein 7
  • Eptotermin alfa
  • OP-1
  • OP-1OP1
  • Osteogenic protein 1


Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3, 4). The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP-7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfide-linked growth factor domains (5, 6). Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4, complexes that show increased potency and range of activity compared to BMP-7 homodimers (7-9). The presence of the propeptides in the BMP-7 tetramer does not diminish the bioactivity of the growth factor domains (6). Secreted BMP-7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix Fibrillin (5). BMP-7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 6). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10, 11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial-mesenchymal transition (EMT) (13-15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuroregeneration following brain ischemia (16).
  1. Carreira, A.C. et al. (2015) J. Dent. Res. 93:335.
  2. Weiskirchen, R. and S.K. Meurer (2013) Front. Biosci. (Landmark Ed.) 18:1407.
  3. Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
  4. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9843.
  5. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
  6. Sengle, G. et al. (2008) J. Mol. Biol. 381:1025.
  7. Israel, D.I. et al. (1996) Growth Factors 13:291.
  8. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  9. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  10. Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352.
  11. Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181.
  12. Grishina, I.B. et al. (2005) Dev. Biol. 288:334.
  13. Zeisberg, M. et al. (2003) Nat. Med. 9:964.
  14. Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047.
  15. Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567.
  16. Chou, J. et al. (2006) J. Neurol. Sci. 240:21.

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Publications for BMP-7 (354-BP/CF)(92)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Porcine, Xenopus, Zebrafish.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), ELISA Capture, ELISA Standard, In Vivo, Surface Plasmon Resonance, Surface Plasmon Resonance (SPR.

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Showing Publications 1 - 10 of 92. Show All 92 Publications.
Publications using 354-BP/CF Applications Species
S Abdul Maje, H Dunzendorf, J Weiner, JT Heiker, W Kiess, A Körner, K Landgraf COBL, MKX and MYOC Are Potential Regulators of Brown Adipose Tissue Development Associated with Obesity-Related Metabolic Dysfunction in Children International Journal of Molecular Sciences, 2023-02-04;24(4):. 2023-02-04 [PMID: 36834493] (Bioassay, Mouse) Bioassay Mouse
R Oude Egber, HM Zegelaar, N El Boujnou, EMM Versteeg, WF Daamen, R Brock Biomaterial-Mediated Protein Expression Induced by Peptide-mRNA Nanoparticles Embedded in Lyophilized Collagen Scaffolds Pharmaceutics, 2022-08-02;14(8):. 2022-08-02 [PMID: 36015245] (ELISA Standard, Mouse) ELISA Standard Mouse
T Hayashi, M Asakura, M Kawase, M Matsubara, Y Uematsu, A Mieki, T Kawai Bone Tissue Engineering in Rat Calvarial Defects Using Induced Bone-like Tissue by rhBMPs from Immature Muscular Tissues In Vitro International Journal of Molecular Sciences, 2022-06-22;23(13):. 2022-06-22 [PMID: 35805943] (Bioassay, Rat) Bioassay Rat
BY Kim, SH Choi, JY Kim, J Ko, JI Yook, HS Kim, EJ Lee, DO Kikkawa, JS Yoon Potential Therapeutic Role of Bone Morphogenic Protein 7 (BMP7) in the Pathogenesis of Graves&#039; Orbitopathy Investigative Ophthalmology & Visual Science, 2022-06-01;63(6):7. 2022-06-01 [PMID: 35671049] (Bioassay, Human) Bioassay Human
J Jeong, TH Kim, M Kim, YK Jung, KS Kim, S Shim, H Jang, WI Jang, SB Lee, D Choi Elimination of Reprogramming Transgenes Facilitates the Differentiation of Induced Pluripotent Stem Cells into Hepatocyte-like Cells and Hepatic Organoids Biology, 2022-03-23;11(4):. 2022-03-23 [PMID: 35453693] (Bioassay, Human) Bioassay Human
S Minatoguch, S Saito, K Furuhashi, Y Sawa, M Okazaki, Y Shimamura, AB Kaihan, Y Hashimoto, Y Yasuda, A Hara, Y Mizutani, R Ando, N Kato, T Ishimoto, N Tsuboi, N Esaki, M Matsuyama, Y Shiraki, H Kobayashi, N Asai, A Enomoto, S Maruyama A novel renal perivascular mesenchymal cell subset gives rise to fibroblasts distinct from classic myofibroblasts Scientific Reports, 2022-03-30;12(1):5389. 2022-03-30 [PMID: 35354870] (Bioassay, Mouse) Bioassay Mouse
J Jansen, KC Reimer, JS Nagai, FS Varghese, GJ Overheul, M de Beer, R Roverts, D Daviran, LAS Fermin, B Willemsen, M Beukenboom, S Djudjaj, S von Stillf, LE van Eijk, M Mastik, M Bulthuis, WD Dunnen, H van Goor, JL Hillebrand, SH Triana, T Alexandrov, MC Timm, BT van den Be, M van den Br, Q Nlandu, J Heijnert, EMJ Bindels, RM Hoogenboez, F Mooren, C Kuppe, P Miesen, K Grünberg, T Ijzermans, EJ Steenberge, J Czogalla, MF Schreuder, N Sommerdijk, A Akiva, P Boor, VG Puelles, J Floege, TB Huber, COVID Moon, RP van Rij, IG Costa, RK Schneider, B Smeets, R Kramann SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids Cell Stem Cell, 2021-12-25;29(2):217-231.e8. 2021-12-25 [PMID: 35032430] (Bioassay, Human) Bioassay Human
Y Soejima, N Iwata, Y Nakano, K Yamamoto, A Suyama, T Nada, F Otsuka Biphasic Roles of Clock Genes and Bone Morphogenetic Proteins in Gonadotropin Expression by Mouse Gonadotrope Cells International Journal of Molecular Sciences, 2021-10-17;22(20):. 2021-10-17 [PMID: 34681844] (Cell Culture, Human) Cell Culture Human
EGJ Ripmeester, MMJ Caron, GGH van den Ak, J Steijns, DAM Surtel, A Cremers, LCW Peeters, LW van Rhijn, TJM Welting BMP7 reduces the fibrocartilage chondrocyte phenotype Scientific Reports, 2021-10-04;11(1):19663. 2021-10-04 [PMID: 34608249] (Bioassay, Human) Bioassay Human
R Kumar, AV Grinberg, H Li, TH Kuo, D Sako, L Krishnan, K Liharska, J Li, R Grenha, MC Maguire, SD Briscoe, RS Pearsall, BR Herrin, RNVS Suragani, R Castonguay Functionally diverse heteromeric traps for ligands of the transforming growth factor-beta superfamily Scientific Reports, 2021-09-15;11(1):18341. 2021-09-15 [PMID: 34526551] (Bioassay, Human) Bioassay Human
Show All 92 Publications.

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Gene Symbol BMP7