Recombinant Human BMP-7 Protein, CF

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Recombinant human BMP-7 (354-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 0.1-0.6 µg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human BMP-7 Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.1-0.6 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-7 protein
Ser293-His431
Accession #
N-terminal Sequence
Ser293
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP7
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
15.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-20 kDa, reducing conditions
Publications
Read Publications using
354-BP/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-7 Protein, CF

  • BMP7
  • BMP-7
  • bone morphogenetic protein 7
  • Eptotermin alfa
  • OP-1
  • OP-1OP1
  • Osteogenic protein 1

Background

Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3, 4). The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP-7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfide-linked growth factor domains (5, 6). Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4, complexes that show increased potency and range of activity compared to BMP-7 homodimers (7-9). The presence of the propeptides in the BMP-7 tetramer does not diminish the bioactivity of the growth factor domains (6). Secreted BMP-7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix Fibrillin (5). BMP-7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 6). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10, 11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial-mesenchymal transition (EMT) (13-15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuroregeneration following brain ischemia (16).
  1. Carreira, A.C. et al. (2015) J. Dent. Res. 93:335.
  2. Weiskirchen, R. and S.K. Meurer (2013) Front. Biosci. (Landmark Ed.) 18:1407.
  3. Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
  4. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9843.
  5. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
  6. Sengle, G. et al. (2008) J. Mol. Biol. 381:1025.
  7. Israel, D.I. et al. (1996) Growth Factors 13:291.
  8. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  9. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  10. Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352.
  11. Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181.
  12. Grishina, I.B. et al. (2005) Dev. Biol. 288:334.
  13. Zeisberg, M. et al. (2003) Nat. Med. 9:964.
  14. Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047.
  15. Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567.
  16. Chou, J. et al. (2006) J. Neurol. Sci. 240:21.

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Publications for BMP-7 (354-BP/CF)(83)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Porcine, Xenopus, Zebrafish.

We have publications tested in 7 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), ELISA Capture, In Vivo, Surface Plasmon Resonance.


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Binding Assay
(2)
Bioassay
(67)
Cell Culture
(3)
ELISA (Capture)
(1)
ELISA Capture
(1)
In Vivo
(6)
Surface Plasmon Resonance
(2)
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Human
(50)
Mouse
(18)
Rat
(4)
Bovine
(2)
Chicken
(3)
Porcine
(1)
Xenopus
(3)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 83. Show All 83 Publications.
Publications using 354-BP/CF Applications Species
Y Soejima, N Iwata, Y Nakano, K Yamamoto, A Suyama, T Nada, F Otsuka Biphasic Roles of Clock Genes and Bone Morphogenetic Proteins in Gonadotropin Expression by Mouse Gonadotrope Cells International Journal of Molecular Sciences, 2021;22(20):. 2021 [PMID: 34681844] (Cell Culture, Human) Cell Culture Human
EGJ Ripmeester, MMJ Caron, GGH van den Ak, J Steijns, DAM Surtel, A Cremers, LCW Peeters, LW van Rhijn, TJM Welting BMP7 reduces the fibrocartilage chondrocyte phenotype Scientific Reports, 2021;11(1):19663. 2021 [PMID: 34608249] (Bioassay, Human) Bioassay Human
R Kumar, AV Grinberg, H Li, TH Kuo, D Sako, L Krishnan, K Liharska, J Li, R Grenha, MC Maguire, SD Briscoe, RS Pearsall, BR Herrin, RNVS Suragani, R Castonguay Functionally diverse heteromeric traps for ligands of the transforming growth factor-beta superfamily Scientific Reports, 2021;11(1):18341. 2021 [PMID: 34526551] (Bioassay, Human) Bioassay Human
L E, R Lu, J Sun, H Li, W Xu, H Xing, X Wang, T Cheng, S Zhang, X Ma, R Zhang, H Liu Microenvironment Influences on Human Umbilical Cord Mesenchymal Stem Cell-Based Bone Regeneration Stem Cells International, 2021;2021(0):4465022. 2021 [PMID: 34447439] (Bioassay, Human) Bioassay Human
T Nakajima, A Nakahata, N Yamada, K Yoshizawa, TM Kato, M Iwasaki, C Zhao, H Kuroki, M Ikeya Grafting of iPS cell-derived tenocytes promotes motor function recovery after Achilles tendon rupture Nature Communications, 2021;12(1):5012. 2021 [PMID: 34408142] (Bioassay, Human) Bioassay Human
S Srinivasa, R Garcia-Mar, M Torriani, KV Fitch, AR Carlson, CR Kahn, SK Grinspoon Altered pattern of circulating miRNAs in HIV lipodystrophy perturb key adipose differentiation and inflammation pathways JCI Insight, 2021;0(0):. 2021 [PMID: 34383714] (Bioassay, Human) Bioassay Human
L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32783886] (Bioassay, Human) Bioassay Human
EGJ Ripmeester, MMJ Caron, GGH van den Ak, DAM Surtel, A Cremers, P Balaskas, P Dyer, BAC Housmans, A Chabronova, A Smagul, Y Fang, LW van Rhijn, MJ Peffers, TJM Welting Impaired chondrocyte U3 snoRNA expression in osteoarthritis impacts the chondrocyte protein translation apparatus Sci Rep, 2020;10(1):13426. 2020 [PMID: 32778764] (Bioassay, Human) Bioassay Human
Kumar Gupta A, Sarkar P, Wertheim J, Pan X, Carroll T, Oxburgh L Asynchronous mixing of kidney progenitor cells potentiates nephrogenesis in organoids. Commun Biol, 0;3(1):231. 0 [PMID: 32393756] (Bioassay, Human) Bioassay Human
A Krouwels, JD Iljas, AHM Kragten, WJA Dhert, FC Öner, MA Tryfonidou, LB Creemers Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration Int J Mol Sci, 2020;21(8):. 2020 [PMID: 32295299] (Bioassay, Human) Bioassay Human
Show All 83 Publications.

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Bioinformatics

Gene Symbol BMP7
Uniprot