354-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED
50 for this effect is 0.1-0.6 µg/mL." class="big_lightbox">

354-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED
50 for this effect is 0.1-0.6 µg/mL." alt="Recombinant human BMP-7 (
354-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED
50 for this effect is 0.1-0.6 µg/mL." class="big_thumb" />
294-HG), Recombinant Human EGF (
236-EG), Recombinant Human FGF-10 (
345-FG), Recombinant Human Noggin (
6057-NG), Recombinant Human R-Spondin 1 (
4645-RS), and Recombinant Human Wnt-3a (
5036-WN), along with the other reagents listed in the liver organoid initiation medium recipe in the
human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Recombinant Human BMP-7 (Catalog # 354-BP), Recombinant Human HGF (
294-HG), Recombinant Human EGF (
236-EG), and Recombinant Human FGF-19 (
969-FG), along with the other reagents listed in the liver organoid differentiation medium recipe in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (
MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (
AF2400; red) and counterstained with DAPI (
5748; blue)." class="big_lightbox">

294-HG), Recombinant Human EGF (
236-EG), Recombinant Human FGF-10 (
345-FG), Recombinant Human Noggin (
6057-NG), Recombinant Human R-Spondin 1 (
4645-RS), and Recombinant Human Wnt-3a (
5036-WN), along with the other reagents listed in the liver organoid initiation medium recipe in the
human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Recombinant Human BMP-7 (Catalog # 354-BP), Recombinant Human HGF (
294-HG), Recombinant Human EGF (
236-EG), and Recombinant Human FGF-19 (
969-FG), along with the other reagents listed in the liver organoid differentiation medium recipe in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (
MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (
AF2400; red) and counterstained with DAPI (
5748; blue)." class="big_thumb" />