BMP-7 Products

354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-7 Monoclonal Antibody (Catalog # MAB3541). The ND₅₀ is typically 1.5-6.0 µg/mL in the presence of L-ascorbic acid (50 µg/mL)." class="big_lightbox" target="_blank">

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542). The ND₅₀ is typically 4-12 µg/mL in the presence of L-ascorbic acid (50 µg/mL)." class="big_lightbox" target="_blank">

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

354-BP) was serially diluted and captured by Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human BMP‑7 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox" target="_blank">

354-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED₅₀ for this effect is 0.1-0.6 µg/mL." class="big_lightbox" target="_blank">

294-HG), Recombinant Human EGF (236-EG), Recombinant Human FGF-10 (345-FG), Recombinant Human Noggin (6057-NG), Recombinant Human R-Spondin 1 (4645-RS), and Recombinant Human Wnt-3a ( 5036-WN), along with the other reagents listed in the liver organoid initiation medium recipe in the human liver organoid culture protocol. After 3 days, liver organoid expansion medium was added for 7-10 days, followed by liver organoid differentiation medium, which includes Recombinant Human BMP-7 (Catalog # 354-BP), Recombinant Human HGF (294-HG), Recombinant Human EGF (236-EG), and Recombinant Human FGF-19 (969-FG), along with the other reagents listed in the liver organoid differentiation medium recipe in the human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (AF2400; red) and counterstained with DAPI (5748; blue)." class="big_lightbox" target="_blank">