New pricing — Effective July 1, 2022 -

Amidst rising costs across all areas of our business, and aggressive attempts to implement cost savings without sacrificing quality, we announce the need to implement a cost increase starting July 1, 2022. If you have questions, please contact your sales representative.

Recombinant Human beta-NGF Protein, CF

Images

 
1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human beta -NGF (Catalog # 256‑GF/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2-2 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human beta-NGF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.2-2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human beta-NGF protein
Ser122-Ala241
Accession #
N-terminal Sequence
Ser122
Protein/Peptide Type
Recombinant Proteins
Gene
NGF
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Publications
Read Publications using
256-GF/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human beta-NGF Protein, CF

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428

Background

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha , beta , and  gamma . Both the alpha and  gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta  subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Customers Who Viewed This Item Also Viewed...

DBD00
Species: Hu
Applications: ELISA
267-N3/CF
Species: Hu
Applications: BA
AF1056
Species: Rt
Applications: IHC, WB
MAB367
Species: Ca, Hu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
236-EG
Species: Hu
Applications: BA
NBP1-30052
Species: Ch, Gp, Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, WB
NBP1-47897
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
233-FB/CF
Species: Hu
Applications: BA
MAB3468
Species: Hu
Applications: ICC, WB
DRT200
Species: Hu
Applications: ELISA
212-GD/CF
Species: Hu
Applications: Bind, BA
H00001523-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
H00003059-M02
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, PLA, S-ELISA, WB
MAB224
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
NBP1-18910
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
AF1138
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut, WB
H00007170-M02
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
256-GF/CF
Species: Hu
Applications: Bioactivity

Publications for beta-NGF (256-GF/CF)(45)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Chicken, N/A, Porcine.

We have publications tested in 7 applications: Bioassay, Cell Culture, Differentiation, ELISA (Capture), ELISA Standard, Western Blot, cell cul.


Filter By Application
Bioassay
(34)
Cell Culture
(3)
Differentiation
(1)
ELISA (Capture)
(1)
ELISA Standard
(1)
Western Blot
(2)
cell cul
(1)
All Applications
Filter By Species
Human
(24)
Mouse
(3)
Rat
(8)
Canine
(2)
Chicken
(3)
N/A
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using 256-GF/CF Applications Species
D Segelcke, HK Fischer, M Hütte, S Dennerlein, F Benseler, N Brose, EM Pogatzki-Z, M Schmidt Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice Cell Reports, 2021;37(12):110152. 2021 [PMID: 34936870] (Bioassay, Mouse) Bioassay Mouse
P Göttle, K Schichel, L Reiche, L Werner, A Zink, A Prigione, P Küry TLR4 Associated Signaling Disrupters as a New Means to Overcome HERV-W Envelope-Mediated Myelination Deficits Frontiers in Cellular Neuroscience, 2021;15(0):777542. 2021 [PMID: 34887730] (Bioassay, Rat) Bioassay Rat
AT Regua, NR Aguayo, SA Jalboush, DL Doheny, SG Manore, D Zhu, GL Wong, A Arrigo, CJ Wagner, Y Yu, A Thomas, MD Chan, J Ruiz, G Jin, R Strowd, P Sun, J Lin, HW Lo TrkA Interacts with and Phosphorylates STAT3 to Enhance Gene Transcription and Promote Breast Cancer Stem Cells in Triple-Negative and HER2-Enriched Breast Cancers Cancers, 2021;13(10):. 2021 [PMID: 34066153] (Bioassay, Human) Bioassay Human
B Sun, X Yang, F Hou, X Yu, Q Wang, HS Oh, P Raja, JM Pesola, EAH Vanni, S McCarron, J Morris-Lov, AHM Ng, GM Church, DM Knipe, DM Coen, D Pan Regulation of host and virus genes by neuronal miR-138 favours herpes simplex virus 1 latency Nature Microbiology, 2021;0(0):. 2021 [PMID: 33558653] (Bioassay, Mouse) Bioassay Mouse
KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri Modulation of Human Adipose Stem Cells&#039; Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 2020;9(9):. 2020 [PMID: 32839392] (Cell Culture, Human) Cell Culture Human
B Xia, J Gao, S Li, L Huang, L Zhu, T Ma, L Zhao, Y Yang, K Luo, X Shi, L Mei, H Zhang, Y Zheng, L Lu, Z Luo, J Huang Mechanical stimulation of Schwann cells promote peripheral nerve regeneration via extracellular vesicle-mediated transfer of microRNA 23b-3p Theranostics, 2020;10(20):8974-8995. 2020 [PMID: 32802175] (Cell Culture, Rat) Cell Culture Rat
A Yoshida, K Takashima, T Shimonaga, M Kadokura, S Nagase, S Koda Establishment of a simple one-step method for oligodendrocyte progenitor cell preparation from rodent brains J. Neurosci. Methods, 2020;0(0):108798. 2020 [PMID: 32479973] (cell cul, Rat) cell cul Rat
JS Ebo, JC Saunders, PWA Devine, AM Gordon, AS Warwick, B Schiffrin, SE Chin, E England, JD Button, C Lloyd, NJ Bond, AE Ashcroft, SE Radford, DC Lowe, DJ Brockwell An in vivo platform to select and evolve aggregation-resistant proteins Nat Commun, 2020;11(1):1816. 2020 [PMID: 32286330] (Bioassay, Human) Bioassay Human
N Uesato, N Miyagawa, K Inagaki, R Kakefuda, Y Kitagawa, Y Matsuo, T Yamaguchi, T Hata, K Ikegashira, M Matsushita Pharmacological Properties of JTE-952, an Orally Available and Selective Colony Stimulating Factor 1 Receptor Kinase Inhibitor Biol. Pharm. Bull., 2020;43(2):325-333. 2020 [PMID: 32009119] (Cell Culture, Human) Cell Culture Human
AR Nickolls, MM Lee, DF Espinoza, M Szczot, RM Lam, Q Wang, J Beers, J Zou, MQ Nguyen, HJ Solinski, AA AlJanahi, KR Johnson, ME Ward, AT Chesler, CG Bönnemann Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells Cell Rep, 2020;30(3):932-946.e7. 2020 [PMID: 31968264] (Differentiation, Human) Differentiation Human
Show All 45 Publications.

Reviews for beta-NGF (256-GF/CF) (0)

There are no reviews for beta-NGF (256-GF/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for beta-NGF (256-GF/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional beta-NGF Products

Bioinformatics Tool for beta-NGF (256-GF/CF)

Discover related pathways, diseases and genes to beta-NGF (256-GF/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for beta-NGF (256-GF/CF)

Discover more about diseases related to beta-NGF (256-GF/CF).
 

Pathways for beta-NGF (256-GF/CF)

View related products by pathway.

PTMs for beta-NGF (256-GF/CF)

Learn more about PTMs related to beta-NGF (256-GF/CF).

Blogs on beta-NGF

There are no specific blogs for beta-NGF, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human beta-NGF Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol NGF
Uniprot