Recombinant Human beta-NGF Protein, CF


Recombinant Human beta -NGF (Catalog # 256‑GF/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2-2 ng/mL.
1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human beta-NGF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.2-2 ng/mL.
Mouse myeloma cell line, NS0-derived human beta-NGF protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13 kDa, reducing conditions
Read Publications using
256-GF/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human beta-NGF Protein, CF

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428


NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha , beta , and  gamma . Both the alpha and  gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta  subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

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Publications for beta-NGF (256-GF/CF)(49)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Chicken, Horse, Porcine.

We have publications tested in 9 applications: Bioassay, Cell Culture, Control, Differentiation, ELISA (Capture), ELISA (Standard), ELISA Standard, In Vivo, Western Blot.

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Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using 256-GF/CF Applications Species
YH Lai, CY Su, HW Cheng, CY Chu, LB Jeng, CS Chiang, WC Shyu, SY Chen Stem cell-nanomedicine system as a theranostic bio-gadolinium agent for targeted neutron capture cancer therapy Nature Communications, 2023-01-18;14(1):285. 2023-01-18 [PMID: 36650171] (Bioassay, Human) Bioassay Human
S Nyström, A Kendall, S Adepu, A Lindahl, E Skiöldebra The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay Research in Veterinary Science, 2022-08-22;151(0):156-163. 2022-08-22 [PMID: 36029605] (Control, Horse) Control Horse
AK Holzer, I Suciu, C Karreman, T Goj, M Leist Specific Attenuation of Purinergic Signaling during Bortezomib-Induced Peripheral Neuropathy In Vitro International Journal of Molecular Sciences, 2022-03-29;23(7):. 2022-03-29 [PMID: 35409095] (Differentiation, Differentiation, Human) Differentiation, Differentiation Human
D Segelcke, HK Fischer, M Hütte, S Dennerlein, F Benseler, N Brose, EM Pogatzki-Z, M Schmidt Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice Cell Reports, 2021-12-21;37(12):110152. 2021-12-21 [PMID: 34936870] (Bioassay, Mouse) Bioassay Mouse
P Göttle, K Schichel, L Reiche, L Werner, A Zink, A Prigione, P Küry TLR4 Associated Signaling Disrupters as a New Means to Overcome HERV-W Envelope-Mediated Myelination Deficits Frontiers in Cellular Neuroscience, 2021-11-23;15(0):777542. 2021-11-23 [PMID: 34887730] (Bioassay, Rat) Bioassay Rat
AT Regua, NR Aguayo, SA Jalboush, DL Doheny, SG Manore, D Zhu, GL Wong, A Arrigo, CJ Wagner, Y Yu, A Thomas, MD Chan, J Ruiz, G Jin, R Strowd, P Sun, J Lin, HW Lo TrkA Interacts with and Phosphorylates STAT3 to Enhance Gene Transcription and Promote Breast Cancer Stem Cells in Triple-Negative and HER2-Enriched Breast Cancers Cancers, 2021-05-12;13(10):. 2021-05-12 [PMID: 34066153] (Bioassay, Human) Bioassay Human
B Sun, X Yang, F Hou, X Yu, Q Wang, HS Oh, P Raja, JM Pesola, EAH Vanni, S McCarron, J Morris-Lov, AHM Ng, GM Church, DM Knipe, DM Coen, D Pan Regulation of host and virus genes by neuronal miR-138 favours herpes simplex virus 1 latency Nature Microbiology, 2021-02-08;0(0):. 2021-02-08 [PMID: 33558653] (Bioassay, Mouse) Bioassay Mouse
KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri Modulation of Human Adipose Stem Cells&#039; Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 2020-08-21;9(9):. 2020-08-21 [PMID: 32839392] (Bioassay, Cell Culture, Human) Bioassay, Cell Culture Human
B Xia, J Gao, S Li, L Huang, L Zhu, T Ma, L Zhao, Y Yang, K Luo, X Shi, L Mei, H Zhang, Y Zheng, L Lu, Z Luo, J Huang Mechanical stimulation of Schwann cells promote peripheral nerve regeneration via extracellular vesicle-mediated transfer of microRNA 23b-3p Theranostics, 2020-07-11;10(20):8974-8995. 2020-07-11 [PMID: 32802175] (Bioassay, Cell Culture, Rat) Bioassay, Cell Culture Rat
H Yu, W Hu, X Song, Y Zhao Generation of Multipotent Stem Cells from Adult Human Peripheral Blood Following the Treatment with Platelet-Derived Mitochondria Cells, 2020-05-29;9(6):. 2020-05-29 [PMID: 32485922] (Bioassay, Human) Bioassay Human
Show All 49 Publications.

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Gene Symbol NGF