Recombinant Human beta-NGF Protein, CF

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1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human beta -NGF (Catalog # 256‑GF/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2-2 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human beta-NGF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.2-2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human beta-NGF protein
Ser122-Ala241
Accession #
N-terminal Sequence
Ser122
Protein/Peptide Type
Recombinant Proteins
Gene
NGF
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Publications
Read Publications using
256-GF/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human beta-NGF Protein, CF

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428

Background

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and  gamma. Both the alpha and  gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta  subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

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Publications for beta-NGF (256-GF/CF)(22)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Chicken, N/A, Porcine.

We have publications tested in 2 applications: Bioassay, WB Ctrl.


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Bioassay
(18)
WB Ctrl
(2)
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Human
(8)
Mouse
(1)
Rat
(3)
Canine
(2)
Chicken
(3)
N/A
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using 256-GF/CF Applications Species
Engineering the surface properties of a human monoclonal antibody prevents self-association and rapid clearance in vivo Sci Rep, 2016;6(0):38644. 2016 [PMID: 27995962] (Bioassay, Rat) Bioassay Rat
Nerve Growth Factor Attenuates Apoptosis and Inflammation in the Diabetic Cornea Invest. Ophthalmol. Vis. Sci, 2016;57(15):6767-6775. 2016 [PMID: 27978558] (Bioassay, Human) Bioassay Human
Hierarchical CRMP2 posttranslational modifications control NaV1.7 function Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):. 2016 [PMID: 27940916] (Bioassay, Human) Bioassay Human
BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer&#039;s disease models Sci Rep, 2016;6(0):33427. 2016 [PMID: 27641902] (Bioassay, Human) Bioassay Human
S Delbary-Go, S Lee, M Baroni, I Lamarche, M Arnone, B Canolle, A Lin, J Sacramento, EA Salegio, MN Castel, N Delesque-T, A Alam, P Laboudie, B Ferzaz, P Savi, JM Herbert, GT Manley, AR Ferguson, JC Bresnahan, F Bono, MS Beattie A novel inhibitor of p75-neurotrophin receptor improves functional outcomes in two models of traumatic brain injury Brain, 2016;0(0):. 2016 [PMID: 27084575] (Bioassay, Human) Bioassay Human
Park K, Lin C, Li K, Lee Y Effects of Reducing Suppressors of Cytokine Signaling-3 (SOCS3) Expression on Dendritic Outgrowth and Demyelination after Spinal Cord Injury. PLoS ONE, 2015;10(9):e0138301. 2015 [PMID: 26384335] (Bioassay, Mouse) Bioassay Mouse
Schwab, Andrew J, Ebert, Allison Sensory neurons do not induce motor neuron loss in a human stem cell model of spinal muscular atrophy. PLoS ONE, 2014;9(7):e103112. 2014 [PMID: 25054590] (Bioassay, Human) Bioassay Human
Wan C, Liu J, Nie X, Zhao J, Zhou S, Duan Z, Tang C, Liang L, Xu G 2, 3, 7, 8-Tetrachlorodibenzo-P-dioxin (TCDD) induces premature senescence in human and rodent neuronal cells via ROS-dependent mechanisms. PLoS ONE, 2014;9(2):e89811. 2014 [PMID: 24587053] (Bioassay, Rat) Bioassay Rat
Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, Sieber-Blum M Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury. Stem Cells Transl Med, 2014;3(3):334-45. 2014 [PMID: 24443004] (Bioassay, Canine) Bioassay Canine
Ko J, Mizuno Y, Ohki C, Chikama T, Sonoda K, Kiuchi Y Neuropeptides released from trigeminal neurons promote the stratification of human corneal epithelial cells. Invest Ophthalmol Vis Sci, 2014;55(1):125-33. 2014 [PMID: 24327615] (Bioassay, Human) Bioassay Human
Show All 22 Publications.

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Bioinformatics

Gene Symbol NGF
Entrez
Uniprot