Recombinant Human beta-NGF Protein, CF


1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human beta -NGF (Catalog # 256‑GF/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2-2 ng/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human beta-NGF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.2-2 ng/mL.
Mouse myeloma cell line, NS0-derived human beta-NGF protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
13.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13 kDa, reducing conditions
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256-GF/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human beta-NGF Protein, CF

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428


NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and  gamma. Both the alpha and  gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta  subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

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Publications for beta-NGF (256-GF/CF)(40)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Chicken, N/A, Porcine.

We have publications tested in 7 applications: Bioassay, Cell Culture, Differentiation, ELISA (Capture), ELISA Standard, Western Blot, cell cul.

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Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using 256-GF/CF Applications Species
KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri Modulation of Human Adipose Stem Cells&#039; Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 2020;9(9):. 2020 [PMID: 32839392] (Cell Culture, Human) Cell Culture Human
B Xia, J Gao, S Li, L Huang, L Zhu, T Ma, L Zhao, Y Yang, K Luo, X Shi, L Mei, H Zhang, Y Zheng, L Lu, Z Luo, J Huang Mechanical stimulation of Schwann cells promote peripheral nerve regeneration via extracellular vesicle-mediated transfer of microRNA 23b-3p Theranostics, 2020;10(20):8974-8995. 2020 [PMID: 32802175] (Cell Culture, Rat) Cell Culture Rat
A Yoshida, K Takashima, T Shimonaga, M Kadokura, S Nagase, S Koda Establishment of a simple one-step method for oligodendrocyte progenitor cell preparation from rodent brains J. Neurosci. Methods, 2020;0(0):108798. 2020 [PMID: 32479973] (cell cul, Rat) cell cul Rat
JS Ebo, JC Saunders, PWA Devine, AM Gordon, AS Warwick, B Schiffrin, SE Chin, E England, JD Button, C Lloyd, NJ Bond, AE Ashcroft, SE Radford, DC Lowe, DJ Brockwell An in vivo platform to select and evolve aggregation-resistant proteins Nat Commun, 2020;11(1):1816. 2020 [PMID: 32286330] (Bioassay, Human) Bioassay Human
N Uesato, N Miyagawa, K Inagaki, R Kakefuda, Y Kitagawa, Y Matsuo, T Yamaguchi, T Hata, K Ikegashira, M Matsushita Pharmacological Properties of JTE-952, an Orally Available and Selective Colony Stimulating Factor 1 Receptor Kinase Inhibitor Biol. Pharm. Bull., 2020;43(2):325-333. 2020 [PMID: 32009119] (Cell Culture, Human) Cell Culture Human
AR Nickolls, MM Lee, DF Espinoza, M Szczot, RM Lam, Q Wang, J Beers, J Zou, MQ Nguyen, HJ Solinski, AA AlJanahi, KR Johnson, ME Ward, AT Chesler, CG Bönnemann Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells Cell Rep, 2020;30(3):932-946.e7. 2020 [PMID: 31968264] (Differentiation, Human) Differentiation Human
B Xia, J Gao, S Li, L Huang, T Ma, L Zhao, Y Yang, J Huang, Z Luo Extracellular Vesicles Derived From Olfactory Ensheathing Cells Promote Peripheral Nerve Regeneration in Rats Front Cell Neurosci, 2019;13(0):548. 2019 [PMID: 31866834] (Bioassay, Rat) Bioassay Rat
EP McCurdy, KM Chung, CR Benitez-Ag, U Hengst Promotion of Axon Growth by the Secreted End of a Transcription Factor Cell Rep, 2019;29(2):363-377.e5. 2019 [PMID: 31597097] (Bioassay, Rat) Bioassay Rat
D Baumeister, W Eich, S Saft, O Geisel, R Hellweg, A Finn, CI Svensson, J Tesarz No evidence for altered plasma NGF and BDNF levels in fibromyalgia patients Sci Rep, 2019;9(1):13667. 2019 [PMID: 31541132] (ELISA Standard, Human) ELISA Standard Human
A Kizilyer, MV Singh, VB Singh, S Suwunnakor, J Palis, SB Maggirwar Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis Sci Rep, 2019;9(1):2781. 2019 [PMID: 30808933] (Bioassay, Human) Bioassay Human
Show All 40 Publications.

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Gene Symbol NGF