Recombinant Human beta IG-H3 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human beta IG-H3 Protein, CF Summary

Details of Functionality
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. rh beta IG-H3, immobilized at 5 µg/mL (100 µL/well), can induce greater than 45% cell adhesion.
Source
Mouse myeloma cell line, NS0-derived human beta IG-H3 protein
Gly24-His683, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Gly24
Protein/Peptide Type
Recombinant Proteins
Gene
TGFBI
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
65-70 kDa, reducing conditions
Publications
Read Publications using
3409-BG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human beta IG-H3 Protein, CF

  • beta IGH3
  • beta IG-H3
  • BIGH3EBMD
  • CDB1
  • CDG2
  • CDGG1transforming growth factor, beta-induced, 68kD
  • CSD
  • CSD1
  • CSD2
  • CSD3
  • Kerato-epithelin
  • LCD1
  • RGD-CAP
  • RGD-containing collagen-associated protein
  • TGFBI
  • TGFBIP
  • transforming growth factor, beta-induced, 68kDa
  • transforming growth factor-beta-induced protein ig-h3

Background

Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation (1-4). The human beta IG-H3 cDNA encodes a 683 amino acid (aa) precursor that includes a 23 aa signal sequence, one EMI domain, four FAS1 domains, and one RGD motif (1). Human beta IG-H3 shares 91% and 93% aa sequence identity with mouse and porcine beta IG-H3, respectively. beta IG-H3 is expressed as a 75 kDa protein with no post-translational additions (5). Following secretion, cleavages at multiple positions near the C-terminal end liberate peptides with pro-apoptotic activity (5,6). Peptides that encompass the RGD motif contribute to the pro-apoptotic effects of TGF-beta (6). FAS1 domains contain YH motifs that are characterized by conserved Tyr and His residues (7). The YH motifs in each of the FAS1 domains enable beta IG-H3 binding to matrix Fibronectin, Collagen I, and Collagen VI (3, 8 - 10) in addition to cell expressed Integrins alpha V beta 3, alpha V beta 5, and alpha 3 beta 1 (7, 8, 11, 12). The expression of beta IG-H3 is modulated at particular developmental stages in some cell types. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts (8, 11, 13). It promotes keratinocyte differentiation but blocks osteoblast differentiation (8,11). beta IG-H3 stimulates macrophage endocytosis and vascular endothelial cell proliferation and migration (12, 13). High glucose levels induce beta IG-H3 in renal proximal tubule cells which is predictive of diabetic nephropathy (3). Several point mutations (clustered in the fourth FAS1 domain) of beta IG-H3 are linked to different corneal dystrophies (14). beta IG-H3 is downregulated in many cancers (4, 15) and functions as a suppressor of tumorigenicity when overexpressed (2, 4, 15).

  1. Skonier, J. et al. (1992) DNA Cell Biol. 11:511.
  2. Skonier, J. et al. (1994) DNA Cell Biol. 13:571.
  3. Lee, S-H. et al. (2003) Kidney Int. 64:1012.
  4. Zhao, Y.L. et al. (2002) Oncogene 21:7471.
  5. Andersen, R.B. et al. (2004) Biochemistry 43:16374.
  6. Kim, J-E. et al. (2003) Oncogene 22:2045.
  7. Kim, J-E. et al. (2002) J. Biol. Chem. 277:46159.
  8. Thapa, N. et al. (2005) Bone 36:232.
  9. Hanssen, E. et al. (2003) J. Biol. Chem. 278:24334.
  10. Billings, P.C. et al. (2002) J. Biol. Chem. 277:28003.
  11. Oh, J-E. et al. (2005) J. Biol. Chem. 280:21629.
  12. Nam, J-O. et al. (2003) J. Biol. Chem. 278:25902.
  13. Cao, W, et al. (2006) Blood 107:2777. 
  14. Stewart, H.S. et al. (1999) Hum. Mutat. 14:126.
  15. Zhao, Y. et al. (2006) Mol. Carcinog. 45:84.

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3409-BG
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Publications for Beta Ig-h3/TGFBI (3409-BG)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol TGFBI
Uniprot