Recombinant Human BAMBI/NMA Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human BAMBI/NMA Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED₅₀ for this effect is 1-6 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human BAMBI/NMA protein
  

  Human BAMBI/NMA (Glu27-Ala152) Accession # Q13145	DIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q13145
• N-terminal Sequence: Glu27
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: BAMBI
• Purity: >90%, by SDS-PAGE with silver staining, under reducing conditions
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 45-48 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE with silver staining, under reducing conditions
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BAMBI/NMA Fc Chimera Protein, CF

• BAMBI
• BMP and activin membrane-bound inhibitor homolog (Xenopus laevis)
• BMP and activin membrane-bound inhibitor homolog
• NMA
• NMANon-metastatic gene A protein
• Putative transmembrane protein NMA

Background

BAMBI (BMP and Activin membrane-bound inhibitor), also called NMA (non-metastatic gene A) is a type I transmembrane protein that shares sequence homology with the TGF‑ beta type I receptor family and functions as a decoy receptor (1). The 260 amino acid (aa) human BAMBI protein contains a 20 aa signal sequence, a 132 aa extracellular domain (ECD) with one potential N‑glycosylation site, a transmembrane domain and a cytoplasmic domain. The human BAMBI ECD shares 91%, 93%, 94%, 94%, 92%, 92% and 92% aa sequence identity with mouse, rat, canine, porcine, equine, ovine and bovine BAMBI, respectively. BAMBI differs from the
TGF-beta  RI family by having a short cytoplasmic region that lacks the kinase domain (1, 2). It competes with type I receptors and forms heterodimers with type II receptors (2). During development, BAMBI is prominent in gastrulation, neurulation, and development of teeth and bones, and is often co‑expressed with BMP family members (1, 2, 4, 6). It antagonizes Activin and TGF‑ beta signaling, and may increase the dynamic range of BMPs to aid in BMP gradient effectiveness (4, 6). In the adult, it modulates processes such as adipogenesis, response to arterial injury, and tumor/tumor stroma promotion by TGF-beta (1, 3, 7, 9‑11). Although it is not expressed in other normal skin cells, it is highly expressed in melanocytes and may be inversely correlated with metastatic potential in melanomas (3). BAMBI is induced by TGF‑ beta and Wnt signaling, and cooperates with inhibitory Smad6 and Smad7 to inhibit TGF‑ beta signaling (5). In contrast, it promotes Wnt signaling (8).

1. Knight, C. et al. (2001) J. Dent. Res. 80:1895.
2. Onichtchouk, D. et al. (1999) Nature 401:480.
3. Degen, W.G. et al. (1996) Int. J. Cancer 65:460.
4. Paulsen, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:10202.
5. Yan, X. et al. (2009) J. Biol. Chem. 284:30097.
6. Montero, J.A. et al. (2008) Dev. Biol. 321:343.
7. Guillot, N. et al. (2013) PLoS ONE 8:e58550.
8. Lin, Z. et al. (2008) J. Biol. Chem. 283:33053.
9. Luo, X. et al. (2012) Diabetes 61:124.
10. Shangguan, L. et al. (2012) Stem Cells 30:2810.
11. Sekiya, T. et al. (2004) J. Biol. Chem. 279:6840.

Publications for BAMBI/NMA (921-BM)(1)

Publication using 921-BM

• Devlin , Anna-Cla, Burr , Karen, Borooah , Shyamang, Foster , Joshua D, Cleary , Elaine M, Geti , Imbisaat, Vallier , Ludovic, Shaw , Christop, Chandran , Siddhart, Miles , Gareth B Human iPSC-derived motoneurons harbouring TARDBP or C9ORF72 ALS mutations are dysfunctional despite maintaining viability. Nat Commun, 2015-01-12;6(0):5999. 2015-01-12 [PMID: 25580746] (Bioassay, Human)

Bioinformatics

• Gene Symbol: BAMBI
• Uniprot:
  • Q13145()