Recombinant Human Adiponectin/Acrp30 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity, Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Adiponectin/Acrp30 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit glucose production by rat hepatocytes. Zhou, H. et al. (2005) Biochem. Biophys. Res. Commun. 338:793.

The ED50 for this effect is 4‑20 µg/mL. Measured by its ability to induce TIMP-1 secretion by mouse macrophages. Kumada, M. et al. (2004) Circulation 109:2046. The ED50 for this effect is 1-5 µg/mL.

Source
Mouse myeloma cell line, NS0-derived human Adiponectin/Acrp30 protein
Glu19-Asn244, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Glu19
Structure / Form
Oligomer
Protein/Peptide Type
Recombinant Proteins
Gene
ADIPOQ
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
25.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
36 kDa, reducing conditions
Publications
Read Publications using
1065-AP in the following applications:

Packaging, Storage & Formulations

Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied as a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Adiponectin/Acrp30 Protein, CF

  • ACDC
  • Acrp30
  • ACRP30ADPN
  • adipocyte, C1Q and collagen domain containing
  • Adiponectin
  • adiponectin, C1Q and collagen domain containing
  • AdipoQ
  • ADIPQTL1
  • ApM1
  • apM-1
  • APM1APM-1
  • C1q and collagen domain-containing protein
  • GBP28
  • GBP28apM1
  • Gelatin-binding protein

Background

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1 - 3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4 - 7). Mature human Adiponectin consists of a 60 amino acid (aa) N‑terminal collagenous region and a 137 aa C‑terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8 - 9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10 - 12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
  9. Waki, H. et al. (2005) Endocrinology 146:790.
  10. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  11. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  12. Wang, Y. et al. (2008) Biochem. J. 409:623.
  13. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  14. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

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Publications for Adiponectin/Acrp30 (1065-AP)(18)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Porcine.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), In Vivo, Western Blot.


Filter By Application
Bioassay
(13)
ELISA (Standard)
(1)
In Vivo
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(11)
Mouse
(2)
Rat
(1)
N/A
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using 1065-AP Applications Species
A Miranda-Ma, OF Mercado-Gó, V Arriaga-Áv, R Guevara-Gu Distribution of Adiponectin Receptors 1 and 2 in the Rat Olfactory Bulb and the Effect of Adiponectin Injection on Insulin Receptor Expression Int J Endocrinol, 2017;2017(0):4892609. 2017 [PMID: 29463982] (In Vivo, Rat) In Vivo Rat
A Rak, K Zajda, E? Gregoraszc Endocrine disrupting compounds modulates adiponectin secretion, expression of its receptors and action on steroidogenesis in ovarian follicle Reprod. Toxicol, 2017;0(0):. 2017 [PMID: 28284726] (Bioassay, Porcine) Bioassay Porcine
K Gasbarrino, H Zheng, A Hafiane, JP Veinot, C Lai, SS Daskalopou Decreased Adiponectin-Mediated Signaling Through the AdipoR2 Pathway Is Associated With Carotid Plaque Instability Stroke, 2017;0(0):. 2017 [PMID: 28258256] (Bioassay, Human) Bioassay Human
S Fu, H Xu, M Gu, C Liu, Q Wang, X Wan, Y Chen, Q Chen, Y Peng, Z Cai, J Zhou, Z Wang Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity Sci Rep, 2017;7(0):43771. 2017 [PMID: 28256562] (Bioassay, Human) Bioassay Human
R Ito, S Narita, M Huang, T Nara, K Numakura, K Takayama, H Tsuruta, A Maeno, M Saito, T Inoue, N Tsuchiya, S Satoh, T Habuchi The impact of obesity and adiponectin signaling in patients with renal cell carcinoma: A potential mechanism for the obesity paradox PLoS ONE, 2017;12(2):e0171615. 2017 [PMID: 28178338] (Bioassay, Human) Bioassay Human
Nokhbehsaim M, Keser S, Nogueira A, Cirelli J, Jepsen S, Jager A, Eick S, Deschner J Beneficial effects of adiponectin on periodontal ligament cells under normal and regenerative conditions. J Diabetes Res, 2014;2014(0):796565. 2014 [PMID: 25121107] (Bioassay, Human) Bioassay Human
Chen H, Tsou H, Chen J, Shih J, Chen Y, Tang C Adiponectin enhances intercellular adhesion molecule-1 expression and promotes monocyte adhesion in human synovial fibroblasts. PLoS ONE, 2014;9(3):e92741. 2014 [PMID: 24667577] (Bioassay, Human) Bioassay Human
Lavoie V, Kernaleguen AE, Charron G, Farhat N, Cossette M, Mamarbachi AM, Allen BG, Rheaume E, Tardif JC Functional effects of adiponectin on endothelial progenitor cells. Obesity (Silver Spring), 2011;19(4):722-8. 2011 [PMID: 20814418] (Bioassay, Human) Bioassay Human
Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010;56(0):1320. 2010 [PMID: 20530731]
von Eynatten M, Liu D, Hock C, Oikonomou D, Baumann M, Allolio B, Korosoglou G, Morcos M, Campean V, Amann K, Lutz J, Heemann U, Nawroth PP, Bierhaus A, Humpert PM Urinary adiponectin excretion: a novel marker for vascular damage in type 2 diabetes. Diabetes, 2009;58(9):2093-9. 2009 [PMID: 19509019] (Western Blot, N/A) Western Blot N/A
Show All 18 Publications.

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Bioinformatics

Gene Symbol ADIPOQ
Uniprot