Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein, CF

When Recombinant Human IL-23 Protein (Catalog # 1290-IL) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Cynomolgus Monkey IL-23R Fc Chimera (Catalog # 10306-IR) that produces 50% of the optimal binding response is found to be approximately 50‑250 ng/mL.

2 μg/lane of Recombinant Cynomolgus Monkey IL-23R Fc Chimera (Catalog # 10306-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 91-102 kDa and 180-200 kDa, respectively.

• Reactivity: Pm-Cm
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL-23 Protein (Catalog # 1290-IL) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey IL-23R Fc Chimera (Catalog # 10306-IR) that produces 50% of the optimal binding response is found to be approximately 50-250 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived cynomolgus monkey IL-23R protein
  

  Cynomolgus Monkey IL-23R (Gly24-Asp353) Accession # XP_005543141	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: XP_005543141
• N-terminal Sequence: Gly24
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 91-102 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein, CF

• IBD17
• IL-23 R
• IL-23 receptor
• IL23R
• IL-23R
• interleukin 23 receptor
• interleukin-23 receptor

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif (6). The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human TH0, TH1 as well as several NK cell lines. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5 (2). The Cynomolgus IL-23 R shares 96%, 71% and 77% amino acid sequence identity to Human, mouse, and rat IL-23 R, respectively.

1. Oppmann, B. et al. (2000) Immunity 13:715.
2. Lankford, C.S. and Frucht, D.M. (2003) J. Leukoc. Biol. 73:49.
3. Parham, C. et al. (2002) J. Immunol. 168:5699.
4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
6. Schroder, J. et al. (2015) J. Biol. Chem. 290:359.

Bioinformatics

• Uniprot:
  • XP_005543141()