Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF

When Recombinant Human Vitronectin (Catalog # 2308-VN) is immobilized at 2 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey Integrin alpha V beta 3 (Catalog # 10426-AV) binds with an ED50 of 0.04‑0.32 μg/mL.

2 μg/lane of Recombinant Cynomolgus Monkey Integrin alpha V beta 3 Protein (Catalog # 10426-AV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-160 kDa.

• Reactivity: Pm-Cm
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA.

  When Recombinant Human Vitronectin (Catalog # 2308-VN) is immobilized at 2 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Integrin alpha V beta 3 (Catalog # 10426-AV) binds with an ED₅₀ of 0.06-0.72 μg/mL.

• Source: Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Integrin alpha V beta 3 protein
  

  Cynomolgus Monkey Integrin alpha V (Phe31-Val992) Accession # XP_005573729.1	His-Pro	2x GGGSGGGS-Acidic Tail	6-His tag
  Cynomolgus Monkey Integrin beta 3 (Gly27-Asp718) Accession # XP_005584667.1	His-Pro	2x GGGSGGGS-Basic tail	HA tag
  N-terminus			C-terminus

• Accession #: XP_005573729.1 (Integrin alpha V) & XP_005584667.1 (Int
• N-terminal Sequence: Phe31 (Integrin alpha V) & Gly27 (Integrin beta 3)
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 115 kDa (Integrin alpha V) & 86 kDa (Integrin beta 3).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-160 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF

• antigen identified by monoclonal L230
• CD51 antigen
• CD51
• Integrin alpha V beta 3
• integrin alpha-V
• integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• MSK8
• Vitronectin receptor subunit alpha
• VNRADKFZp686A08142

Background

Integrin alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (1­3). The non­covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2­4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N­terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta­propeller structure (4). The alpha V subunit contributes a "thigh and a calf" region, while the beta 3 subunit contains a PSI domain and four cysteine­rich I­EGF folds. The alpha V subunit domains termed thigh, calf­1 and calf­2 generate a "knee" region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by "inside out" signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Within the extracellular domain (ECD), cynomolgus alpha V shares 99% amino acid (aa) sequence identity with the 962 aa human alpha V, while cynomolgus beta 3 ECD shares almost 100% aa sequence identity with the 692 aa human beta 3 ECD. Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone, as well as promotes their apoptosis (8, 9). M­CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Cell entry of several viruses is mediated by alpha V beta 3 (4, 10). alpha V beta 3 is involved in several other signaling pathways by direct interaction with receptor tyrosine kinases and ligands. For example, it cooperates with endothelial cell VEGF R2 in angiogenesis, and with IGF­1 to promote cancer cell proliferation and invasiveness (11, 12).

1. Hynes, R. O. (2002) Cell 110:673.
2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
4. Xiong, J. et al. (2001) Science 294:339.
5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
7. Djaffar, I. et al. (1994) Biochem. J. 300:69.
8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
11. Somanath, P.R. et al. (2009) Angiogenesis 12:177.
12. Saegusa, J. et al. (2009) J. Biol. Chem. 284:24106.

Publications for Integrin alpha V beta 3 (10426-AV)(1)

Publication using 10426-AV

• A Bugatti, F Filippini, S Messali, M Giovanetti, C Ravelli, A Zani, M Ciccozzi, A Caruso, F Caccuri The D405N Mutation in the Spike Protein of SARS-CoV-2 Omicron BA.5 Inhibits Spike/Integrins Interaction and Viral Infection of Human Lung Microvascular Endothelial Cells Viruses, 2023-01-24;15(2):. 2023-01-24 [PMID: 36851546] (SPR, Primate (Chlorocebus aethiops))

Bioinformatics

• Uniprot:
  • XP_005573729.1 (Integrin alpha V) & XP_005584667.1 (Int()