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Recombinant Cyno/Rhesus L-Selectin/CD62L Fc Protein, CF

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Recombinant Cynomolgus Monkey/Rhesus Macaque L‑Selectin/CD62L Fc Chimera Protein (Catalog # 11192-LS) supports the adhesion LS180 human colorectal adenocarcinoma cells. The ED50 for this effect is 0.300-3.60 μg/mL.
2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque L‑Selectin/CD62L Fc Chimera Protein (Catalog # 11192-LS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by ...read more

Product Details

Summary
Reactivity Pm-Cm, RMSpecies Glossary
Format
Carrier-Free

Order Details

Recombinant Cyno/Rhesus L-Selectin/CD62L Fc Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells. The ED50 for this effect is 0.300-3.60 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived L-Selectin/CD62L protein
Recombinant Cyno/Rhesus L-Selectin
(Trp52-Asn345)
Accession # XP_005539996.1
IEGRMDHuman IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Trp52
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
85-95 kDa, under reducing conditions.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cyno/Rhesus L-Selectin/CD62L Fc Protein, CF

  • CD62L antigen
  • CD62L
  • gp90-MEL
  • hLHRc
  • LAM1
  • LAM-1
  • LAM1LECAM1
  • LECAM1
  • LEU8
  • Leu-8
  • Leukocyte adhesion molecule 1
  • Leukocyte surface antigen Leu-8
  • Leukocyte-endothelial cell adhesion molecule 1
  • LNHR
  • LNHRTQ1
  • LSEL
  • L-Selectin
  • LYAM1
  • Lyam-1
  • LYAM1CD62 antigen-like family member L
  • Lymph node homing receptor
  • lymphocyte adhesion molecule 1
  • pln homing receptor
  • PLNHR
  • selectin L
  • SELL
  • TQ1

Background

L-Selectin, also known as Leukocyte adhesion molecule 1 (LAM‑1) and CD62L, is a type-1 cell surface glycoprotein and cell adhesion molecule of the Selectin family (1). In humans, there are 3 Selectins, P, E, and L, and they are Ca2+ dependent lectins that help mediate the initial adhesive step during inflammation and immune surveillance (2). Mature L-Selectin consists of an extracellular domain (ECD) with a C-type lectin domain and an epidermal growth factor (EGF)-like domain, a transmembrane domain, and a short cytoplasmic domain. Within the ECD, cynomolgus L-Selectin shares 94% amino acid sequence identity with human L-Selectin. Several isoforms arising from alternative splicing have been reported, some with potential therapeutic implications (2,3). L-selectin is constitutively expressed on a wide variety of leukocytes and plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites (1-4). Acting in cooperation with P-Selectin and E-Selectin, L‑Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium (5). This initial interaction, also involving ICAM-1 and VCAM-1, leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue (6). L-selectin function is required for normal Treg cell migration and over expression might be result in reduced tumor growth (7). A number of studies have reported that levels of L‑Selectin may be elevated or lowered in subjects with a variety of conditions, such as in Alzheimer's disease or rheumatoid arthritis (3, 5).
  1. Ivetic, A. et al. (2019) Front. Immunol. 10:1068.
  2. Grailer, J.J. et al. (2009) J. Dermatol sci. 56:141.
  3. Hirata, T. et al. (2015) Biochem Biophys Res. Commun. 462:371.
  4. Wedepohl, S. et al. (2012) Euro. J. Cell Biol. 91:257.
  5. Ivetic, A. et al. (2013) Inter. J. Biochem. Cell Biol. 45:550.
  6. Granger, D.N. and Senchenkova, E. (2010) Inflammation and the Microcirculation. San Rafael (CA): Morgan & Claypool Life Sciences Chapter 7.
  7. Watson, H.A. et al. (2019) Frontiers in immunology 10:1321.

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L-selectin is a member of the selectin family of glycoprotein adhesion and homing receptors that recognize sialyated carbohydrate groups and regulate lymphocyte-endothelial cell interactions. It is a type I transmembrane cell adhesion molecule (CAM) a...  Read full blog post.

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