Recombinant Canine HGF Protein

• Reactivity: Ca
• Applications: Binding Activity, Bioactivity

Recombinant Canine HGF Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using GGE canine gallbladder epithelial cells. The ED₅₀ for this effect is 10-40 ng/mL.

  Measured by its ability to compete with biotinylated Recombinant Canine HGF for binding to immobilized Recombinant Canine HGF R/c-MET (Catalog # 4140-ME) in a functional ELISA.

  
  Optimal dilutions should be determined by each laboratory for each application.

• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived canine HGF protein
  Arg28-Arg494 (Lys266Gln, Trp321Arg, Leu458Pro) ( alpha chain) & Val495-Ser730 (Ile693Thr) ( beta chain)
• Accession #: Q867B7
• N-terminal Sequence: Arg28 ( alpha chain) & Val495 ( beta chain)
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: HGF
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
  • Bioactivity
• SDS-PAGE: 32 kDa and 56 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Canine HGF Protein

• deafness, autosomal recessive 39
• DFNB39
• EC 3.4.21
• EC 3.4.21.7
• fibroblast-derived tumor cytotoxic factor
• F-TCF
• hepatocyte growth factor (hepapoietin A; scatter factor)
• Hepatopoeitin-A
• Hepatopoietin A
• HGF
• HGFB
• HPTA
• HPTAhepatocyte growth factor
• lung fibroblast-derived mitogen
• Scatter factor
• SF
• SFhepatopoeitin-A

Background

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1 - 4). Canine HGF is secreted as an inactive 699 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta  chain. A variant of HGF with a 5 aa deletion in the first Kringle domain has been described in canine and other species (5). Canine HGF shares 97%, 99%, 93%, 93%, and 89% aa sequence identity with bovine, feline, human, mouse, and rat HGF, respectively. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF‑dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
5. Miyake, M. et al. (2003) Vet. Immunol. Immunopathol. 95:135.
6. Mizuno, K. et al. (1994) J. Biol. Chem. 269:1131.
7. Neo, S. et al. (2005) J. Vet. Med. Sci. 67:525.
8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
10. Montesano, R. et al. (1991) Cell 67:901.
11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
14. Dremier, S. et al. (1994) Endocrinology 135:135.
15. Linke, A. et al. (2005) Proc. Natl. Acad. Sci. 102:8966.

Publications for HGF (3386-HG)(2)

Publications using 3386-HG

• E Bresso, A Furlan, P Noel, V Leroux, F Maina, R Dono, B Maigret Large-Scale Virtual Screening Against the MET Kinase Domain Identifies a New Putative Inhibitor Type Molecules, 2020-02-19;25(4):. 2020-02-19 [PMID: 32093126] (Cell Culture, Canine)
• Matsumoto , Shinji, Fujii , Shinsuke, Sato , Akira, Ibuka , Souji, Kagawa , Yoshinor, Ishii , Masaru, Kikuchi , Akira A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J, 2014-02-20;33(7):702-18. 2014-02-20 [PMID: 24562386] (Bioassay, Canine)

Bioinformatics

• Gene Symbol: HGF
• Uniprot:
  • Q867B7()