RCAN1 Antibody - BSA Free

Western Blot: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN in human fetal brain

Immunohistochemistry: RCAN1 Antibody [NBP1-46853] - Analysis of RCAN1 in human fetal brain

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RCAN1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RCAN1 Antibody - BSA Free (NBP1-46853) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-RCAN1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide made to an internal portion of human RCAN1 (within residues 210-252). [Swiss-Prot# P53805]
• Localization: Cytoplasm, nucleus
• Predicted Species: Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RCAN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 34272844)
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 2 ug/ml
• Application Notes: In WB a band is seen at ~38 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Smad7 Antibody (3G8) - Azide and BSA Free
  Recombinant Human SUN1 GST (N-Term) Protein
  Recombinant Human SUN1 GST (N-Term) Protein

• Control Peptide:

  RCAN1 Peptide (NBP1-46853PEP)

Reactivity Notes

Immunogen has 92% identity to rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RCAN1 Antibody - BSA Free

• ADAPT78
• calcipressin-1
• calcium and oxidant-inducible mRNA
• Down syndrome candidate region 1
• Down syndrome critical region gene 1
• Down syndrome critical region protein 1
• DSC1
• modulatory calcineurin-interacting protein 1
• Myocyte-enriched calcineurin-interacting protein 1
• near DSCR proline-rich protein
• regulator of calcineurin 1CSP1DSCR1MCIP1RCN1

Background

RCAN1 (regulator of calcineurin 1) inhibits the calcineurin-dependent transcriptional responses by binding to catalytic domain of calcineurin 1, a protein phosphatase involved in muscle cell differentiation/growth, T cell activation and new memory generation. Unphosphorylated RCAN1 binds/inhibits calcineurin, while RCAN1 phosphorylation at conserved serine residues interefere with this binding, resulting in increased calcineurin activity. RCAN1 over-expression mediated reduction in calcineurin activity alters the phosphorylation/activity of several calcineurin targets such as Munc18, dynamin 1, amphiphysin 1/2 and synaptojanin which are involved in exocytosis and vesicle recycling. Chronic alterations in calcineurin lead to transcriptional effects via calcineurin/NFAT signalling that regulates pre-synaptic neurotransmitter release. RCAN1 also affect exocytosis and quantal release kinetics via direct calcineurin independent mechanisms. RCAN1 is widely expressed in normal tissues/cell types with highest expression in neurons, and RCAN1 levels elevates in Down syndrome fetuses as well as Alzheimer patients, whereas, in Huntington disease RCAN1 - expression is found reduced. RCAN1 has been implicated in a variety of cellular processes, including oxidative stress, angiogenesis, mitochondrial function, immune responses and inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RCAN1 Antibody (NBP1-46853)(2)

Publications using NBP1-46853

• Ding HX, Dong NX, Zhou CX et al. Liraglutide alleviates vascular injury in diabetic rabbits with lower limb vascular stenosis through regulation of RCAN1 European review for medical and pharmacological sciences 2022-06-01 [PMID: 35731069] (WB, Rabbit)
• Koh J, Zhang R, Roman S Et Al. Ex vivo intact tissue analysis reveals alternative calcium-sensing behaviors in parathyroid adenomas The Journal of clinical endocrinology and metabolism 2021-07-17 [PMID: 34272844] (ICC/IF)

Bioinformatics

• Gene Symbol: RCAN1
• Entrez:
  • 1827(Human)
  • 54720(Mouse)
• Uniprot:
  • P53805(Human)
  • Q9JHG6(Mouse)