RBPMS Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - Mouse intestine. RBPMS stained by RBPMS antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: RBPMS Antibody [NBP2-20112] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (NBP2-20112) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse lymph node.RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: RBPMS Antibody [NBP2-20112] - RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: RBPMS Antibody [NBP2-20112] - RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

RBPMS antibody detects RBPMS protein by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (-) and transfected (+) HCT-116 whole cell extract were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (NBP2-20112) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

RBPMS antibody detects RBPMS protein by immunohistochemical analysis. Samples: Paraffin-embedded mouse retina.Green: RBPMS protein stained by RBPMS antibody (NBP2-20112) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (NBP2-20112) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (NBP2-20112) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody (NBP2-20112) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

RBPMS antibody detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat lymph node.RBPMS stained by RBPMS antibody (NBP2-20112) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, Pm, Bv
• Applications: WB, Flow, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RBPMS Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit RBPMS Antibody - BSA Free (NBP2-20112) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-RBPMS Antibody: Cited in 20 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RBPMS. The exact sequence is proprietary.
• Predicted Species: Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBPMS
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Validated from a verified customer review.
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Whole-Mount Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RBPMS Antibody - Azide and BSA Free

• FLJ32971
• Heart and RRM expressed sequence
• HERMES
• HERMESRNA-binding protein with multiple splicing
• RBPMS
• RBP-MS
• RNA binding protein with multiple splicing

Background

This gene encodes a member of the RRM family of RNA-binding proteins. The RRM domain is between 80-100 amino acids in length and family members contain one to four copies of the domain. The RRM domain consists of two short stretches of conserved sequence called RNP1 and RNP2, as well as a few highly conserved hydrophobic residues. The protein encoded by this gene has a single, putative RRM domain in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBPMS Antibody (NBP2-20112)(23)

Publications using NBP2-20112

• Warwick AM, Bomze HM, Wang L et al. Hypoxia-mediated rescue of retinal ganglion cells deficient in mitochondrial complex I is independent of the hypoxia-inducible factor pathway Scientific Reports 2024-10-15 [PMID: 39406814]
• Wang Q, Lin X, Wang J An optimized method for retrograde labelling and quantification of rabbit retinal ganglion cells Experimental eye research 2023-02-22 [PMID: 36822495] (IF/ICC)
• Warwick AM, Bomze HM, Wang L et al. Continuous Hypoxia Reduces Retinal Ganglion Cell Degeneration in a Mouse Model of Mitochondrial Optic Neuropathy Investigative ophthalmology & visual science 2022-12-01 [PMID: 36538003] (IHC, Mouse)
• Meng Xuan, Wei Wang, Gabriella Bulloch, Jian Zhang, Jason Ha, Qilin Wang, Juanjuan Wang, Xingyan Lin, Mingguang He Impact of Acute Ocular Hypertension on Retinal Ganglion Cell Loss in Mice Translational Vision Science & Technology 2024-03-20 [PMID: 38506800]
• Yifei Li, Dongchang Xiao, Haiqiao Chen, X.F. Steven Zheng, Mengqing Xiang Maf1 controls retinal neuron number by both RNA Pol III- and Pol II-dependent mechanisms iScience 2023-11-23 [PMID: 38089586]
• Want A, Nan X, Kokkali E et al. Brain-derived neurotrophic factor released from blood platelets prevents dendritic atrophy of lesioned adult central nervous system neurons Brain communications 2023-03-02 [PMID: 36970044] (IHC, Mouse)
• John S, Williams P Treatment and prevention of ocular neurodegenerative disorder United States Patent Application 20180344719 2018 Dec 6 (IF, Mouse)
• Li P, Shi X, Liu H et al. HOCPCA Exerts Neuroprotection on Retinal Ganglion Cells by Binding to CaMKII alpha and Modulating Oxidative Stress and Neuroinflammation in Experimental Glaucoma Neuroscience bulletin 2025-06-02 [PMID: 40457155]
• Xinyu Gu, Xun Chen, Xuan Zhang, Keli Liu, Jing-Jing Li, Wenyu Lv, Lei Zeng, Mingjuan Wu, Weibin Zhou, Weifa Wang, Shunhua Shi, Yicheng Deng, Yunhua Li, Xinbo Gao, Rong Ju, Alexandre Dubrac, Xialin Liu, Feng Zhang Macrophage-induced integrin signaling promotes Schlemm's canal formation to prevent intraocular hypertension and glaucomatous optic neuropathy. Cell reports 2024-03-04 [PMID: 38367239]
• Liang Q, Cheng X, Wang J et al. A multi-omics atlas of the human retina at single-cell resolution Cell Genomics 2023-06-14 [PMID: 37388908] (Immunofluorescence, Mouse)
• Rutigliani C, Tribble JR, HagstrOm A et al. Widespread retina and optic nerve neuroinflammation in enucleated eyes from glaucoma patients Acta neuropathologica communications 2022-08-19 [PMID: 35986368] (IHC-P, Human)
  
  Details:
  Dilution used 1:400, optic nerve head sections
• Vohra R, Sanz-Morello B, Tams ALM et al. Prevention of Cell Death by Activation of Hydroxycarboxylic Acid Receptor 1 (GPR81) in Retinal Explants Cells 2022-07-02 [PMID: 35805182] (IHC-WhMt, Mouse)
• D Chen, C Peng, XM Ding, Y Wu, CJ Zeng, L Xu, WY Guo Interleukin-4 promotes microglial polarization toward a neuroprotective phenotype after retinal ischemia/reperfusion injury Oncogene, 2022-12-01;17(12):2755-2760. 2022-12-01 [PMID: 35662225] (Immunofluorescence, Mouse)
• Wang L, Klingeborn M, Travis AM et al. Progressive optic atrophy in a retinal ganglion cell-specific mouse model of complex I deficiency Sci Rep 2020-10-01 [PMID: 33004958] (IF/IHC, Mouse)
• Soo-Ho Choi, Keun-Young Kim, Guy A. Perkins, Sébastien Phan, Genea Edwards, Yining Xia, Jungsu Kim, Dorota Skowronska-Krawczyk, Robert N. Weinreb, Mark H. Ellisman, Yury I. Miller, Won-Kyu Ju AIBP protects retinal ganglion cells against neuroinflammation and mitochondrial dysfunction in glaucomatous neurodegeneration Redox Biology 2020-08-27 [PMID: 32896719]
• Edwards G, Perkins G A et al. Loss of AKAP1 triggers Drp1 dephosphorylation-mediated mitochondrial fission and loss in retinal ganglion cells. Cell Death Dis 2020-04-20 [PMID: 32312949] (IF/IHC, Mouse)
• Williams P, Harder J, Foxworth N et al. Nicotinamide and WLDS Act Together to Prevent Neurodegeneration in Glaucoma Front Neurosci. 2017-04-25 [PMID: 28487632] (IF/IHC, Mouse)
• Williams P, Braine C, Foxworth N et al. GlyCAM1 negatively regulates monocyte entry into the optic nerve head and contributes to radiation-based protection in glaucoma J Neuroinflammation 2017-04-26 [PMID: 28446179] (IF, Mouse)
• Liang Q, Cheng X, Wang J et al. A multi-omics atlas of the human retina at single-cell resolution Cell Genomics 2023-04-01 (Immunohistochemistry, Human)
• Xiao D, Jin K, Qiu S Et al. In vivo Regeneration of Ganglion Cells for Vision Restoration in Mammalian Retinas Frontiers in cell and developmental biology 2021-10-04 [PMID: 34671605] (IHC-P, Mouse)
• Tribble JR, Otmani A, Kokkali E et al. Retinal Ganglion Cell Degeneration in a Rat Magnetic Bead Model of Ocular Hypertensive Glaucoma Translational Vision Science & Technology 2021-01-12 [PMID: 33510960] (Immunofluorescence, Mouse)
• Xiao D, Qiu S, Huang X et al. Directed robust generation of functional retinal ganglion cells from Muller glia Front Physiol 2019-05-30 [PMID: 31139088]

Review for RBPMS Antibody (NBP2-20112) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Patricia Semedo-Kuriki Flow Mouse 04/09/2021

Summary

• Application: Flow Cytometry
• Sample Tested: mouse retina
• Species: Mouse
• Lot: 44153

Bioinformatics

• Gene Symbol: RBPMS