Rat VEGF Quantikine ELISA Kit

• Reactivity: Rt
• Applications: ELISA
• Conjugate: HRP

Rat VEGF Quantikine ELISA Kit Summary

• Background: The Quantikine Rat VEGF Immunoassay is a 3.5 hour solid-phase ELISA designed to measure rat VEGF in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat VEGF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat VEGF showed linear curves that we...re parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat VEGF.
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• Specificity: Natural and recombinant rat VEGF. This assay cross-reacts 59% with Mouse VEGF₁₂₀ (E.Coli-expressed), 73% with Mouse VEGF₁₆₄ (Sf21-expressed), 14% with Canine VEGF₁₆₄ (E.Coli-expressed), 26% with Human VEGF₁₂₁ (Sf21-expressed), 31% with Human VEGF₁₂₁ (E.Coli-expressed), 24% with Mouse VEGF₁₆₂ (NS0-expressed), 0.2% with Human VEGF₂₀₆ (E.Coli-expressed) and 5.2% with Human VEGF/PIGF.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Vegfa

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat VEGF Quantikine ELISA Kit

• MVCD1
• VAS
• vascular endothelial growth factor A
• Vascular permeability factor
• Vasculotropin
• VEGF
• VEGFA
• VEGF-A
• VEGFMGC70609
• VPF
• VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and in adults (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and formation of anti-parallel disulfide-linked dimers (4). Alternately spliced isoforms of 120, 164, and 188 amino acids (aa) have been found in rats and mice, while 121, 145, 165, 183, 189, and 206 aa isoforms have been identified in humans (2, 4). In humans, VEGF₁₆₅ appears to be the most abundant and potent isoform, followed by VEGF₁₂₁ and VEGF₁₈₉ (3, 4). The same pattern may exist in rats and mice. Isoforms other than VEGF₁₂₀ and VEGF₁₂₁ contain basic heparin-binding regions and are not freely diffusible (4). Rat VEGF164 shares 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 89% with porcine and canine, and 90% with feline and equine VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle (5, 6), hepatocytes (7), osteoblasts (8), neutrophils (9), macrophages (10), keratinocytes (11), brown adipose tissue (12), CD34⁺ stem cells (13), endothelial cells (14), fibroblasts, and vascular smooth muscle cells (15). VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M, and TNF-alpha (3, 4, 9). The isoforms are differentially expressed during development and in the adult (3). 

VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) and induce their homodimerization and autophosphorylation (3, 4, 7, 16, 17). These receptors have seven extracellular immunoglobulin-like domains and an intracellular split tyrosine kinase domain. They are expressed on vascular endothelial cells and a range of non-endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF₁₆₅ also binds the semaphorin receptor, neuropilin-1, which promotes complex formation with VEGF R2 (18). 

VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells (3, 4), thus regulating blood vessel density and size but playing no role in determining vascular patterns. VEGF promotes bone formation through osteoblast and chondroblast recruitment and is also a monocyte chemoattractant (19-21). In postnatal life, VEGF maintains endothelial cell integrity and is a potent mitogen for micro- and macro-vascular endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). In diseased tissues, VEGF promotes vascular permeability. It is thus thought to contribute to tumor metastasis by promoting both extravasation and tumor angiogenesis (22, 23). Various strategies have been employed therapeutically to antagonize VEGF-mediated tumor angiogenesis (24). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (25).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for VEGF (RRV00)(76)

Publications using RRV00

• Haba, D;Qin, Q;Takizawa, C;Tomida, S;Minematsu, T;Sanada, H;Nakagami, G; Local low-frequency vibration accelerates healing of full-thickness wounds in a hyperglycemic rat model Journal of diabetes investigation 2023-09-08 [PMID: 37688317] (Rat)
• Tammam, BMH;Habotta, OA;El-Khadragy, M;Abdel Moneim, AE;Abdalla, MS; Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms Heliyon 2023-09-01 [PMID: 37662797] (Rat)
• Acosta, FM;Pacelli, S;Rathbone, CR; Diabetes diminishes muscle precursor cell-mediated microvascular angiogenesis PloS one 2023-08-04 [PMID: 37540699] (Rat)
• Y Iso, S Usui, H Suzuki Mesenchymal Stem/Stromal Cells in Skeletal Muscle Are Pro-Angiogenic, and the Effect Is Potentiated by Erythropoietin Pharmaceutics, 2023-03-24;15(4):. 2023-03-24 [PMID: 37111534] (Rat)
• S Nishida, Y Takashima, R Udagawa, H Ibaraki, Y Seta, H Ishihara A Multifunctional Hybrid Nanocarrier for Non-Invasive siRNA Delivery to the Retina Pharmaceutics, 2023-02-11;15(2):. 2023-02-11 [PMID: 36839933] (Rat)
• W Ji, Z Wu, J Wen, H Tang, Z Chen, B Xue, Z Tian, Y Ba, N Zhang, X Wen, B Hou A simple method to isolate structurally and chemically intact brain vascular basement membrane for neural regeneration following traumatic brain injury Biomaterials research, 2023-01-12;27(1):2. 2023-01-12 [PMID: 36635718] (Rat)
• OAR Abo-Zaid, FSM Moawed, HA Hassan, EM Moustafa Hydrogen sulfide attenuates lung injury instigated by Bisphenol-A via suppressing inflammation and oxidative stress Bmc Pharmacology & Toxicology, 2022-12-30;23(1):98. 2022-12-30 [PMID: 36585682] (Rat)
• A Rezaei, Y Li, M Turmaine, S Bertazzo, CA Howard, TR Arnett, K Shakib, G Jell Hypoxia mimetics restore bone biomineralisation in hyperglycaemic environments Scientific Reports, 2022-08-17;12(1):13944. 2022-08-17 [PMID: 35977987] (Rat)
• SY Ahn, DK Sung, YS Chang, WS Park Intratracheal Transplantation of Mesenchymal Stem Cells Attenuates Hyperoxia-Induced Microbial Dysbiosis in the Lungs, Brain, and Gut in Newborn Rats International Journal of Molecular Sciences, 2022-06-13;23(12):. 2022-06-13 [PMID: 35743045] (Rat)
• X Xu, D Wang, Z Han, B Wang, W Gao, Y Fan, F Li, Z Zhou, C Gao, J Xiong, S Zhou, S Zhang, G Yang, R Jiang, J Zhang A novel rat model of chronic subdural hematoma: Induction of inflammation and angiogenesis in the subdural space mimicking human-like features of progressively expanding hematoma Brain research bulletin, 2021-04-28;172(0):108-119. 2021-04-28 [PMID: 33932488] (Rat)
• S Isozaki, H Konishi, M Fujiya, H Tanaka, Y Murakami, S Kashima, K Ando, N Ueno, K Moriichi, T Okumura Probiotic-Derived Polyphosphate Accelerates Intestinal Epithelia Wound Healing through Inducing Platelet-Derived Mediators Mediators of Inflammation, 2021-03-29;2021(0):5582943. 2021-03-29 [PMID: 33859537] (Rat)
• YY Shin, SM An, JS Jeong, SY Yang, GS Lee, EJ Hong, EB Jeung, SC Kim, BS An Comparison of steroid hormones in�three�different preeclamptic models Molecular Medicine Reports, 2021-02-04;23(4):. 2021-02-04 [PMID: 33537808] (Human, Rat)
• I Maldonado-, N O'Neill, O Umland, J Verhaagen, M Oudega Macrophage-Derived Inflammation Induces a Transcriptome Makeover in Mesenchymal Stromal Cells Enhancing Their Potential for Tissue Repair International Journal of Molecular Sciences, 2021-01-14;22(2):. 2021-01-14 [PMID: 33466704] (Rat)
• W El Bakly, M Medhat, M Shafei, R Tash, M Elrefai, Y Shoukry, NN Omar Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis Heliyon, 2020-09-21;6(9):e05006. 2020-09-21 [PMID: 33005806] (Rat)
• KD Beharry, CL Cai, F Siddiqui, C D'Agrosa, A Zangaladze, G Mustafa, A Qadri, TJ Duggan, JV Aranda Combination Antioxidant/NSAID Therapies and Oral/Topical Ocular Delivery Modes for Prevention of Oxygen-Induced Retinopathy in a Rat Model Nutrients, 2020-07-03;12(7):. 2020-07-03 [PMID: 32635350] (Rat)
• Z Sun, Y Xie, RJ Lee, Y Chen, Q Jin, Q Lv, J Wang, Y Yang, Y Li, Y Cai, R Wang, Z Han, L Zhang, M Xie Myocardium-targeted transplantation of PHD2 shRNA-modified bone mesenchymal stem cells through ultrasound-targeted microbubble destruction protects the heart from acute myocardial infarction Theranostics, 2020-04-06;10(11):4967-4982. 2020-04-06 [PMID: 32308762] (Rat)
• KK Poh, PSS Lee, AH Djohan, MJ Galupo, GG Songco, TC Yeo, HC Tan, AM Richards, L Ye Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4 Cells, 2020-04-12;9(4):. 2020-04-12 [PMID: 32290541] (Rat)
• S Guzel, CL Cai, T Ahmad, M Quan, GB Valencia, JV Aranda, KD Beharry Bumetanide Suppression of Angiogenesis in a Rat Model of Oxygen-Induced Retinopathy Int J Mol Sci, 2020-02-02;21(3):. 2020-02-02 [PMID: 32024231] (Rat)
• Z Zhang, R Qu, T Fan, J Ouyang, F Lu, J Dai Stepwise Adipogenesis of Decellularized Cellular Extracellular Matrix Regulates Adipose Tissue-Derived Stem Cell Migration and Differentiation Stem Cells Int, 2019-11-06;2019(0):1845926. 2019-11-06 [PMID: 31781233] (Rat)
• M Hatakeyama, M Kanazawa, I Ninomiya, K Omae, Y Kimura, T Takahashi, O Onodera, M Fukushima, T Shimohata A novel therapeutic approach using peripheral blood mononuclear cells preconditioned by oxygen-glucose deprivation Sci Rep, 2019-11-14;9(1):16819. 2019-11-14 [PMID: 31728010] (Human)
• CC Hsiao, CC Lin, YS Hou, JY Ko, CJ Wang Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model Int J Mol Sci, 2019-10-05;20(19):. 2019-10-05 [PMID: 31590394] (Rat)
• J Cao, R Yang, TE Smith, S Evans, GW McCollum, SC Pomerantz, T Petley, IR Harris, JS Penn Human Umbilical Tissue-Derived Cells Secrete Soluble VEGFR1 and Inhibit Choroidal Neovascularization Mol Ther Methods Clin Dev, 2019-05-22;14(0):37-46. 2019-05-22 [PMID: 31276010] (Rat)
• M Pérez-Mato, R Iglesias-R, A Vieites-Pr, A Dopico-Lóp, B Argibay, H Fernández-, A da Silva-C, A Pérez-Díaz, C Correa-Paz, A Günther, P Ávila-Góme, M Isabel Loz, A Baumann, J Castillo, T Sobrino, F Campos Blood glutamate EAAT2-cell grabbing therapy in cerebral ischemia EBioMedicine, 2018-12-13;0(0):. 2018-12-13 [PMID: 30555045] (Rat)
• K Kin, T Yasuhara, M Kameda, Y Tomita, M Umakoshi, K Kuwahara, I Kin, N Kidani, J Morimoto, M Okazaki, T Sasaki, N Tajiri, CV Borlongan, I Date Cell encapsulation enhances antidepressant effect of the mesenchymal stem cells and counteracts depressive-like behavior of treatment-resistant depressed rats Mol. Psychiatry, 2018-08-14;0(0):. 2018-08-14 [PMID: 30108315]
• M Ahmed, G Kumar, S Gourevitch, T Levchenko, E Galun, V Torchilin, SN Goldberg Radiofrequency ablation (RFA) induced systemic tumor growth can be reduced by suppression of resultant heat shock proteins Int J Hyperthermia, 2018-04-24;0(0):1-25. 2018-04-24 [PMID: 29631466] (Rat)
• KD Beharry, CL Cai, T Ahmad, S Guzel, GB Valencia, JV Aranda Impact of Chronic Neonatal Intermittent Hypoxia on Severity of Retinal Damage in a Rat Model of Oxygen-Induced Retinopathy J Nat Sci, 2018-01-01;4(3):. 2018-01-01 [PMID: 29552637] (Rat)
• JD Koerner, DZ Markova, GD Schroeder, BP Calio, A Shah, CW Brooks, AR Vaccaro, DG Anderson, CK Kepler The local cytokine and growth factor response to rhBMP-2 after spinal fusion Spine J, 2018-03-14;0(0):. 2018-03-14 [PMID: 29550606] (ELISA (Standard), Rat)
• L Melly, G Cerino, A Frobert, S Cook, MN Giraud, T Carrel, HT Tevaearai, F Eckstein, B Rondelet, A Marsano, A Banfi Myocardial infarction stabilization by cell-based expression of controlled Vascular Endothelial Growth Factor levels J. Cell. Mol. Med., 2018-02-25;0(0):. 2018-02-25 [PMID: 29478261] (Rat)
• M Dworacka, G Chukanova, S Iskakova, Y Kurmambaye, A Weso?owska, BA Frycz, PP Jagodzi?sk, G Dworacki New arguments for beneficial effects of alpha-lipoic acid on the cardiovascular system in the course of type 2 diabetes Eur J Pharm Sci, 2018-02-07;117(0):41-47. 2018-02-07 [PMID: 29427700] (Rat)
• S Cai, Q Yang, M Hou, Q Han, H Zhang, J Wang, C Qi, Q Bo, Y Ru, W Yang, Z Gu, R Wei, Y Cao, X Li, Y Zhang ?-Melanocyte-Stimulating Hormone Protects Early Diabetic Retina from Blood-Retinal Barrier Breakdown and Vascular Leakage via MC4R Cell. Physiol. Biochem., 2018-01-25;45(2):505-522. 2018-01-25 [PMID: 29402864] (Rat)
• C Chen, H Li, J Jiang, Q Zhang, F Yan Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats Oncotarget, 2017-08-14;8(42):72676-72699. 2017-08-14 [PMID: 29069818] (Rat)
• K Hara, C Hamada, K Wakabayash, R Kanda, K Kaneko, S Horikoshi, Y Tomino, Y Suzuki Scavenging of reactive oxygen species by astaxanthin inhibits epithelial-mesenchymal transition in high glucose-stimulated mesothelial cells PLoS ONE, 2017-09-19;12(9):e0184332. 2017-09-19 [PMID: 28926603] (Rat)
• SC Joachim, M Renner, J Reinhard, C Theiss, C May, S Lohmann, S Reinehr, G Stute, A Faissner, K Marcus, HB Dick Protective effects on the retina after ranibizumab treatment in an ischemia model PLoS ONE, 2017-08-11;12(8):e0182407. 2017-08-11 [PMID: 28800629] (Rat)
• MM Nowacka-Ch, M Paul-Samoj, AM Bielecka-W, JJ Barski, E Obuchowicz Alterations in VEGF expression induced by antidepressant drugs in female rats under chronic social stress Exp Ther Med, 2017-01-02;13(2):723-730. 2017-01-02 [PMID: 28352358] (Rat)
• S Becker, H Wang, B Yu, R Brown, X Han, RH Lane, ME Hartnett Protective effect of maternal uteroplacental insufficiency on oxygen-induced retinopathy in offspring: removing bias of premature birth Sci Rep, 2017-02-14;7(0):42301. 2017-02-14 [PMID: 28195189] (Rat)
• Mesenchymal stem cells in corneal neovascularization: Comparison of different application routes Mol Med Rep, 2016-08-11;0(0):. 2016-08-11 [PMID: 27514011] (Rat)
• Sung D, Chang Y, Ahn S, Sung S, Yoo H, Choi S, Kim S, Park W Optimal Route for Human Umbilical Cord Blood-Derived Mesenchymal Stem Cell Transplantation to Protect Against Neonatal Hyperoxic Lung Injury: Gene Expression Profiles and Histopathology. PLoS ONE, 2015-08-25;10(8):e0135574. 2015-08-25 [PMID: 26305093] (Rat)
• Ahmed M, Kumar G, Navarro G, Wang Y, Gourevitch S, Moussa M, Rozenblum N, Levchenko T, Galun E, Torchilin V, Goldberg S Systemic siRNA Nanoparticle-Based Drugs Combined with Radiofrequency Ablation for Cancer Therapy. PLoS ONE, 2015-07-08;10(7):e0128910. 2015-07-08 [PMID: 26154425] (Rat)
• Garrido P, Ribeiro S, Fernandes J, Vala H, Bronze-da-Rocha E, Rocha-Pereira P, Belo L, Costa E, Santos-Silva A, Reis F Iron-hepcidin dysmetabolism, anemia and renal hypoxia, inflammation and fibrosis in the remnant kidney rat model. PLoS ONE, 2015-04-13;10(4):e0124048. 2015-04-13 [PMID: 25867633] (Rat)
• Zhou L, Jang K, Moon Y, Wagle S, Kim K, Lee K, Park B, Kim J Leptin ameliorates ischemic necrosis of the femoral head in rats with obesity induced by a high-fat diet. Sci Rep, 2015-03-23;5(0):9397. 2015-03-23 [PMID: 25797953] (Rat)
• Di Pietro M, Parborell F, Irusta G, Pascuali N, Bas D, Bianchi M, Tesone M, Abramovich D Metformin regulates ovarian angiogenesis and follicular development in a female polycystic ovary syndrome rat model. Endocrinology, 2015-01-15;156(4):1453-63. 2015-01-15 [PMID: 25590243] (Rat)
• Jin C, Yu S, Wang X, Woo S, Park H, Lee H, Choi S, Kim K, Kim J, Park K, Jang H, Lim S The effect of lithospermic acid, an antioxidant, on development of diabetic retinopathy in spontaneously obese diabetic rats. PLoS ONE, 2014-06-06;9(6):e98232. 2014-06-06 [PMID: 24905410] (Rat)
• Bai X, Li X, Tian J, Zhou Z Antiangiogenic treatment diminishes renal injury and dysfunction via regulation of local AKT in early experimental diabetes. PLoS ONE, 2014-04-23;9(4):. 2014-04-23 [PMID: 24759991] (Rat)
• Klinkhammer B, Kramann R, Mallau M, Makowska A, van Roeyen C, Rong S, Buecher E, Boor P, Kovacova K, Zok S, Denecke B, Stuettgen E, Otten S, Floege J, Kunter U Mesenchymal stem cells from rats with chronic kidney disease exhibit premature senescence and loss of regenerative potential. PLoS ONE, 2014-03-25;9(3):e92115. 2014-03-25 [PMID: 24667162] (Rat)
• Jiang Y, Wang H, Culp D, Yang Z, Fotheringham L, Flannery J, Hammond S, Kafri T, Hartnett M Targeting Muller cell-derived VEGF164 to reduce intravitreal neovascularization in the rat model of retinopathy of prematurity. Invest Ophthalmol Vis Sci, 2014-02-10;55(2):824-31. 2014-02-10 [PMID: 24425851] (Rat)
• Terzuoli E, Meini S, Cucchi P, Catalani C, Cialdai C, Maggi C, Giachetti A, Ziche M, Donnini S Antagonism of bradykinin B2 receptor prevents inflammatory responses in human endothelial cells by quenching the NF-kB pathway activation. PLoS ONE, 2014-01-02;9(1):e84358. 2014-01-02 [PMID: 24392129] (Rat)
• Peng Y, Huang S, Wu Y, Cheng B, Nie X, Liu H, Ma K, Zhou J, Gao D, Feng C, Yang S, Fu X Platelet rich plasma clot releasate preconditioning induced PI3K/AKT/NFkappaB signaling enhances survival and regenerative function of rat bone marrow mesenchymal stem cells in hostile microenvironments. Stem Cells Dev, 2013-08-30;22(24):3236-51. 2013-08-30 [PMID: 23885779] (Rat)
• Tong N, Zhang Z, Zhang W, Qiu Y, Gong Y, Yin L, Qiu Q, Wu X Diosmin alleviates retinal edema by protecting the blood-retinal barrier and reducing retinal vascular permeability during ischemia/reperfusion injury. PLoS ONE, 2013-04-24;8(4):e61794. 2013-04-24 [PMID: 23637907] (Rat)
• Nunes S, Soares E, Fernandes J, Viana S, Carvalho E, Pereira F, Reis F Early cardiac changes in a rat model of prediabetes: brain natriuretic peptide overexpression seems to be the best marker. Cardiovasc Diabetol, 2013-03-07;12(0):44. 2013-03-07 [PMID: 23497124] (Rat)
• McCloskey M, Wang H, Jiang Y, Smith G, Strange J, Hartnett M Anti-VEGF antibody leads to later atypical intravitreous neovascularization and activation of angiogenic pathways in a rat model of retinopathy of prematurity. Invest Ophthalmol Vis Sci, 2013-03-21;54(3):2020-6. 2013-03-21 [PMID: 23449716] (Rat)
• Takehara , Yuji, Yabuuchi , Akiko, Ezoe , Kenji, Kuroda , Tomoko, Yamadera , Rie, Sano , Chiaki, Murata , Nana, Aida , Takuya, Nakama , Ken, Aono , Fumihito, Aoyama , Naoki, Kato , Keiich, Kato , Osamu The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function. Lab Invest, 2012-11-19;93(2):181-93. 2012-11-19 [PMID: 23212100] (Rat)
• Abramovich D, Irusta G, Bas D, Cataldi NI, Parborell F, Tesone M Angiopoietins/TIE2 system and VEGF are involved in ovarian function in a DHEA rat model of polycystic ovary syndrome. Endocrinology, 2012-05-10;153(7):3446-56. 2012-05-10 [PMID: 22577112] (Rat)
• Seabrook TJ, Littlewood-Evans A, Brinkmann V, Pollinger B, Schnell C, Hiestand PC Angiogenesis is present in experimental autoimmune encephalomyelitis and pro-angiogenic factors are increased in multiple sclerosis lesions. J Neuroinflammation, 2010-12-22;7(0):95. 2010-12-22 [PMID: 21176212] (Rat)
• Ruiz de Almodovar C, Coulon C, Salin PA, Knevels E, Chounlamountri N, Poesen K, Hermans K, Lambrechts D, Van Geyte K, Dhondt J, Dresselaers T, Renaud J, Aragones J, Zacchigna S, Geudens I, Gall D, Stroobants S, Mutin M, Dassonville K, Storkebaum E, Jordan BF, Eriksson U, Moons L, D'Hooge R, Haigh JJ, Belin MF, Schiffmann S, Van Hecke P, Gallez B, Vinckier S, Chedotal A, Honnorat J, Thomasset N, Carmeliet P, Meissirel C Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1. J. Neurosci., 2010-11-10;30(45):15052-66. 2010-11-10 [PMID: 21068311] (Rat)
• Abarbanell AM, Wang Y, Herrmann JL Toll-like receptor 2 mediates mesenchymal stem cell-associated myocardial recovery and VEGF production following acute ischemia-reperfusion injury. Am. J. Physiol. Heart Circ. Physiol., 2010-02-19;298(5):H1529-36. 2010-02-19 [PMID: 20173040] (Mouse, Rat)
• Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt. Apoptosis, 2010-04-01;15(4):463-73. 2010-04-01 [PMID: 19997978] (Rat)
• Drel VR, Xu W, Zhang J, Pavlov IA, Shevalye H, Slusher B, Obrosova IG Poly(Adenosine 5'-diphosphate-ribose) polymerase inhibition counteracts multiple manifestations of experimental type 1 diabetic nephropathy. Endocrinology, 2009-10-23;150(12):5273-83. 2009-10-23 [PMID: 19854869] (Rat)
• Byfield G, Budd S, Hartnett ME The role of supplemental oxygen and JAK/STAT signaling in intravitreous neovascularization in a ROP rat model. Invest. Ophthalmol. Vis. Sci., 2009-03-05;50(7):3360-5. 2009-03-05 [PMID: 19264880] (Rat)
• Ma P, Luo Y, Zhu X, Ma H, Hu J, Tang S Phosphomannopentaose sulfate (PI-88) inhibits retinal leukostasis in diabetic rat. Biochem. Biophys. Res. Commun., 2009-01-23;380(2):402-6. 2009-01-23 [PMID: 19250642] (Rat)
• Rocha FG, Sundback CA, Krebs NJ, Leach JK, Mooney DJ, Ashley SW, Vacanti JP, Whang EE The effect of sustained delivery of vascular endothelial growth factor on angiogenesis in tissue-engineered intestine. Biomaterials, 2008-04-08;29(19):2884-90. 2008-04-08 [PMID: 18396329] (Rat)
• Valladares D, Quezada C, Montecinos P, Concha II, Yanez AJ, Sobrevia L, San Martin R Adenosine A(2B) receptor mediates an increase on VEGF-A production in rat kidney glomeruli. Biochem. Biophys. Res. Commun., 2007-12-03;366(1):180-5. 2007-12-03 [PMID: 18060864] (Rat)
• Yoo MH, Yoon YH, Chung H, Cho KS, Koh JY Insulin increases retinal hemorrhage in mild oxygen-induced retinopathy in the rat: inhibition by riluzole. Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5671-6. 2007-12-01 [PMID: 18055818] (Rat)
• Yeh WL, Lin CJ, Fu WM Enhancement of glucose transporter expression of brain endothelial cells by vascular endothelial growth factor derived from glioma exposed to hypoxia. Mol. Pharmacol., 2007-10-17;73(1):170-7. 2007-10-17 [PMID: 17942749] (Rat)
• Kim YH, Choi MY, Kim YS, Park CH, Lee JH, Chung IY, Yoo JM, Choi WS, Cho GJ, Kang SS Triamcinolone acetonide protects the rat retina from STZ-induced acute inflammation and early vascular leakage. Life Sci., 2007-08-31;81(14):1167-73. 2007-08-31 [PMID: 17881007] (Rat)
• Crisostomo PR, Markel TA, Wang M, Lahm T, Lillemoe KD, Meldrum DR In the adult mesenchymal stem cell population, source gender is a biologically relevant aspect of protective power. Surgery, 2007-08-01;142(2):215-21. 2007-08-01 [PMID: 17689688] (Rat)
• Yeh WL, Lu DY, Lin CJ, Liou HC, Fu WM Inhibition of hypoxia-induced increase of blood-brain barrier permeability by YC-1 through the antagonism of HIF-1alpha accumulation and VEGF expression. Mol. Pharmacol., 2007-05-18;72(2):440-9. 2007-05-18 [PMID: 17513385] (Rat)
• Li W, Ma N, Ong LL, Nesselmann C, Klopsch C, Ladilov Y, Furlani D, Piechaczek C, Moebius JM, Lutzow K, Lendlein A, Stamm C, Li RK, Steinhoff G Bcl-2 engineered MSCs inhibited apoptosis and improved heart function. Stem Cells, 2007-05-03;25(8):2118-27. 2007-05-03 [PMID: 17478584] (Rat)
• Johansson M, Jansson L, Carlsson PO Improved vascular engraftment and function of autotransplanted pancreatic islets as a result of partial pancreatectomy in the mouse and rat. Diabetologia, 2007-04-04;50(6):1257-66. 2007-04-04 [PMID: 17406853] (Rat)
• Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy. Am. J. Physiol. Heart Circ. Physiol., 2007-03-16;293(1):H474-81. 2007-03-16 [PMID: 17369464] (Rat)
• Kanda Y, Watanabe Y Nicotine-induced vascular endothelial growth factor release via the EGFR-ERK pathway in rat vascular smooth muscle cells. Life Sci., 2007-01-17;80(15):1409-14. 2007-01-17 [PMID: 17286987] (Rat)
• Coughlan MT, Thallas-Bonke V, Pete J, Long DM, Gasser A, Tong DC, Arnstein M, Thorpe SR, Cooper ME, Forbes JM Combination therapy with the advanced glycation end product cross-link breaker, alagebrium, and angiotensin converting enzyme inhibitors in diabetes: synergy or redundancy? Endocrinology, 2006-11-16;148(2):886-95. 2006-11-16 [PMID: 17110423] (Rat)
• Werdich XQ, Penn JS Specific involvement of SRC family kinase activation in the pathogenesis of retinal neovascularization. Invest. Ophthalmol. Vis. Sci., 2006-11-01;47(11):5047-56. 2006-11-01 [PMID: 17065526] (Rat)
• Noh H, Kim JS, Han KH, Lee GT, Song JS, Chung SH, Jeon JS, Ha H, Lee HB Oxidative stress during peritoneal dialysis: implications in functional and structural changes in the membrane. Kidney Int., 2006-06-01;69(11):2022-8. 2006-06-01 [PMID: 16641917] (Rat)
• Iemitsu M, Maeda S, Jesmin S, Otsuki T, Miyauchi T Exercise training improves aging-induced downregulation of VEGF angiogenic signaling cascade in hearts. Am. J. Physiol. Heart Circ. Physiol., 2006-04-14;291(3):H1290-8. 2006-04-14 [PMID: 16617130] (Rat)
• Namisaki T, Yoshiji H, Kojima H, Yoshii J, Ikenaka Y, Noguchi R, Sakurai S, Yanase K, Kitade M, Yamazaki M, Asada K, Uemura M, Nakamura M, Fukui H Salvage effect of the vascular endothelial growth factor on chemically induced acute severe liver injury in rats. J. Hepatol., 2005-09-23;44(3):568-75. 2005-09-23 [PMID: 16310882] (Rat)
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FAQs for VEGF (RRV00). (Showing 1 - 2 of 2 FAQs).

1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
   • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.
2. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: Vegfa