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Rat IL-10 Quantikine ELISA Kit


The box plots of the IL-10 (interleukine-10) level (pg per mg of protein) assessed in rat liver homogenates after 6 weeks of experiment, stratified by the alcoholic beverage. Image collected and cropped by CiteAb from more

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Rat IL-10 Quantikine ELISA Kit Summary

The Quantikine Rat IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-10 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-10 accurately. Results obtained using natural rat IL-10 s...howed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-10.
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Natural and recombinant rat IL-10
Assay Type
Solid Phase Sandwich ELISA
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See PDF Datasheet for details
Spike Recovery
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Sample Volume
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Application Notes
No significant interference observed with available related molecules.
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R1000 in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IL-10 Quantikine ELISA Kit

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF


Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature rat IL-10 shares 85% amino acid sequence identity with mouse IL-10 and 71% - 79% with bovine, canine, equine, feline, guinea pig, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21), and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 have been found to be elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels have been found to be decreased in the serum of patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for IL-10 (R1000)(65)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Rat.

We have publications tested in 1 application: ELISA (Standard).

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Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using R1000 Applications Species
Rajizadeh, MA;Khoramipour, K;Joukar, S;Darvishzadeh-Mahani, F;Iranpour, M;Bejeshk, MA;Zaboli, MD; Lung molecular and histological changes in type 2 diabetic rats and its improvement by high-intensity interval training BMC pulmonary medicine 2024-01-17 [PMID: 38233819] (Rat) Rat
Arafat, MAM;Khalil, MNA;Mohamed, OG;Abd El-Ghafar, OAM;Tripathi, A;Mahrous, EA;Abd El-Kader, EM;El-Hawary, S; Vetiver aerial parts and roots ameliorate rheumatoid arthritis in complete Freund's adjuvant rat model, a phytochemical profiling and mechanistic study Journal of ethnopharmacology 2023-06-13 [PMID: 37315650] (Rat) Rat
MFP Gomes, EOC de Moura, NM Cardoso, GA da Silva, ACC Dos Santos, FS de Souza, D Estadella, RH Lambertucc, JHG Lago, A Medeiros Supplementation with okra�combined or not with exercise training is able to protect the heart of animals with metabolic syndrome Scientific Reports, 2023-01-26;13(1):1468. 2023-01-26 [PMID: 36702820] (Rat) Rat
J Wang, Z Ye, Y Chen, X Qiao, Y Jin MicroRNA-25-5p negatively regulates TXNIP expression and relieves inflammatory responses of brain induced by lipopolysaccharide Scientific Reports, 2022-10-26;12(1):17915. 2022-10-26 [PMID: 36289253] (Rat) Rat
GE Bollar, JD Keith, AM Oden, MR Kiedrowski, SE Birket Acute Infection with a Tobramycin-Induced Small Colony Variant of Staphylococcus aureus Causes Increased Inflammation in the Cystic Fibrosis Rat Lung Infection and Immunity, 2022-09-27;0(0):e0023722. 2022-09-27 [PMID: 36165627] (Transgenic Rat) Transgenic Rat
MM da Silva, MFP Gomes, EOC de Moura, MM Veras, MC Kubota, AP Takano, ACC Dos Santos, CGDR José, GADS Souza, NM Cardoso, D Estadella, RH Lambertucc, A Medeiros Aerobic exercise training combined or not with okra consumption as a strategy to prevent kidney changes caused by metabolic syndrome in Zucker rats PLoS ONE, 2022-06-03;17(6):e0269418. 2022-06-03 [PMID: 35657982] (Rat) Rat
CC Lee, SY Chen, TM Lee 17beta-Oestradiol facilitates M2 macrophage skewing and ameliorates arrhythmias in ovariectomized female infarcted rats Journal of Cellular and Molecular Medicine, 2022-05-05;0(0):. 2022-05-05 [PMID: 35514058] (Rat) Rat
HH Arab, SA Abd El-Aal, AH Eid, EA Arafa, AM Mahmoud, AM Ashour Targeting inflammation, autophagy, and apoptosis by troxerutin attenuates methotrexate-induced renal injury in rats International immunopharmacology, 2021-12-23;103(0):108284. 2021-12-23 [PMID: 34953450] (Rat) Rat
RVP Neves, HL Corrêa, IV de Sousa N, MK Souza, F Costa, AS Haro, LA Deus, AL Reis, HG Simões, RV Andrade, CO Assumpção, W Stone, J Prestes, EC Vieira, R de Cássia, V Cruzat, TS Rosa Renoprotection Induced by Aerobic Training Is Dependent on Nitric Oxide Bioavailability in Obese Zucker Rats Oxidative Medicine and Cellular Longevity, 2021-09-28;2021(0):3683796. 2021-09-28 [PMID: 34621463] (Rat) Rat
M Li, Z Zheng Protective effect of parecoxib sodium against ischemia reperfusion?induced intestinal injury Molecular Medicine Reports, 2021-09-09;24(5):. 2021-09-09 [PMID: 34498709] (Rat) Rat
Show All 65 Publications.

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Gene Symbol Il10