Novus Biologicals products are now on bio-techne.com

Rat IL-10 Quantikine ELISA Kit

Images

 
The box plots of the IL-10 (interleukine-10) level (pg per mg of protein) assessed in rat liver homogenates after 6 weeks of experiment, stratified by the alcoholic beverage. Image collected and cropped by CiteAb from ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Rat IL-10 Quantikine ELISA Kit Summary

Background
The Quantikine Rat IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-10 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-10 accurately. Results obtained using natural rat IL-10 s...howed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-10.
Show More
Specificity
Natural and recombinant rat IL-10
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il10

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
R1000 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IL-10 Quantikine ELISA Kit

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature rat IL-10 shares 85% amino acid sequence identity with mouse IL-10 and 71% - 79% with bovine, canine, equine, feline, guinea pig, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21), and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 have been found to be elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels have been found to be decreased in the serum of patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

Customers Who Viewed This Item Also Viewed...

M6000B
Species: Mu
Applications: ELISA
6507-IL/CF
Species: Hu
Applications: BA
202-IL
Species: Hu
Applications: BA
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
M5000
Species: Mu
Applications: ELISA
7268-CT
Species: Hu
Applications: BA
DY413
Species: Mu
Applications: ELISA
201-LB
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
MAB208
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
NBP1-72042
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NB100-39002
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
NBP2-25208
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
MAB140
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
DCP00
Species: Hu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-10 (R1000)(65)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Rat.

We have publications tested in 1 application: ELISA (Standard).


Filter By Application
ELISA (Standard)
(1)
All Applications
Filter By Species
Mouse
(3)
Rat
(61)
Transgenic Rat
(1)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using R1000 Applications Species
Rajizadeh, MA;Khoramipour, K;Joukar, S;Darvishzadeh-Mahani, F;Iranpour, M;Bejeshk, MA;Zaboli, MD; Lung molecular and histological changes in type 2 diabetic rats and its improvement by high-intensity interval training BMC pulmonary medicine 2024-01-17 [PMID: 38233819] (Rat) Rat
Arafat, MAM;Khalil, MNA;Mohamed, OG;Abd El-Ghafar, OAM;Tripathi, A;Mahrous, EA;Abd El-Kader, EM;El-Hawary, S; Vetiver aerial parts and roots ameliorate rheumatoid arthritis in complete Freund's adjuvant rat model, a phytochemical profiling and mechanistic study Journal of ethnopharmacology 2023-06-13 [PMID: 37315650] (Rat) Rat
MFP Gomes, EOC de Moura, NM Cardoso, GA da Silva, ACC Dos Santos, FS de Souza, D Estadella, RH Lambertucc, JHG Lago, A Medeiros Supplementation with okra�combined or not with exercise training is able to protect the heart of animals with metabolic syndrome Scientific Reports, 2023-01-26;13(1):1468. 2023-01-26 [PMID: 36702820] (Rat) Rat
J Wang, Z Ye, Y Chen, X Qiao, Y Jin MicroRNA-25-5p negatively regulates TXNIP expression and relieves inflammatory responses of brain induced by lipopolysaccharide Scientific Reports, 2022-10-26;12(1):17915. 2022-10-26 [PMID: 36289253] (Rat) Rat
GE Bollar, JD Keith, AM Oden, MR Kiedrowski, SE Birket Acute Infection with a Tobramycin-Induced Small Colony Variant of Staphylococcus aureus Causes Increased Inflammation in the Cystic Fibrosis Rat Lung Infection and Immunity, 2022-09-27;0(0):e0023722. 2022-09-27 [PMID: 36165627] (Transgenic Rat) Transgenic Rat
MM da Silva, MFP Gomes, EOC de Moura, MM Veras, MC Kubota, AP Takano, ACC Dos Santos, CGDR José, GADS Souza, NM Cardoso, D Estadella, RH Lambertucc, A Medeiros Aerobic exercise training combined or not with okra consumption as a strategy to prevent kidney changes caused by metabolic syndrome in Zucker rats PLoS ONE, 2022-06-03;17(6):e0269418. 2022-06-03 [PMID: 35657982] (Rat) Rat
CC Lee, SY Chen, TM Lee 17beta-Oestradiol facilitates M2 macrophage skewing and ameliorates arrhythmias in ovariectomized female infarcted rats Journal of Cellular and Molecular Medicine, 2022-05-05;0(0):. 2022-05-05 [PMID: 35514058] (Rat) Rat
HH Arab, SA Abd El-Aal, AH Eid, EA Arafa, AM Mahmoud, AM Ashour Targeting inflammation, autophagy, and apoptosis by troxerutin attenuates methotrexate-induced renal injury in rats International immunopharmacology, 2021-12-23;103(0):108284. 2021-12-23 [PMID: 34953450] (Rat) Rat
RVP Neves, HL Corrêa, IV de Sousa N, MK Souza, F Costa, AS Haro, LA Deus, AL Reis, HG Simões, RV Andrade, CO Assumpção, W Stone, J Prestes, EC Vieira, R de Cássia, V Cruzat, TS Rosa Renoprotection Induced by Aerobic Training Is Dependent on Nitric Oxide Bioavailability in Obese Zucker Rats Oxidative Medicine and Cellular Longevity, 2021-09-28;2021(0):3683796. 2021-09-28 [PMID: 34621463] (Rat) Rat
M Li, Z Zheng Protective effect of parecoxib sodium against ischemia reperfusion?induced intestinal injury Molecular Medicine Reports, 2021-09-09;24(5):. 2021-09-09 [PMID: 34498709] (Rat) Rat
Show All 65 Publications.

Reviews for IL-10 (R1000) (0)

There are no reviews for IL-10 (R1000). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-10 (R1000) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-10 Products

Blogs on IL-10.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Rat IL-10 Quantikine ELISA Kit and receive a gift card or discount.

Bioinformatics

Gene Symbol Il10