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Rat IL-10 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Rat IL-10 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il10

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DY522 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IL-10 DuoSet ELISA, 15 Plate

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 alpha -helical cytokine family that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-3). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (4, 5). Mature rat IL-10 shares 85% amino acid sequence identity with mouse IL-10 and 71% - 79% with bovine, canine, equine, feline, guinea pig, human, ovine, and porcine IL-10. It contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently-associated homodimer (4, 6, 7). 
IL-10 mediates its biological activities through a heteromeric receptor complex composed of the type II cytokine receptor subunits IL-10 R alpha and IL-10 R beta . IL-10 R alpha is a 110 kDa transmembrane glycoprotein that is expressed on lymphocytes, NK cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells (8-13), while the 75 kDa transmembrane IL-10 R beta is widely expressed (14, 15). The IL-10 dimer binds to two IL-10 R alpha chains, triggering recruitment of two IL-10 R beta chains (14, 15). IL-10 R beta does not bind IL-10 directly but is required for signal transduction. IL-10 R beta also associates with IL-20 R alpha , IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (16-18). 
The involvement of IL-10 in immunoregulation includes both suppressive and stimulatory effects. It functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells (19-21), and by promoting the development of M2 macrophages (21). Its expression by immunosuppressive regulatory T cells (Treg) and regulatory B cells is important for Treg proliferation (19). Within a tumor microenvironment, however, IL-10 inhibits the expansion of Treg as well as myeloid-derived suppressor cells (22, 23). IL-10 induces the intratumoral accumulation and activation of CD8+ T cells (24, 25). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation (19) and promoting muscle regeneration after injury (21), but it also contributes to the persistence of viral infections (26). The levels of IL-10 have been found to be elevated in Sjogren's syndrome (saliva), primary CNS lymphoma (cerebrospinal fluid), and ovarian cancer (serum and ascites) (27-29). Its levels have been found to be decreased in the serum of patients with recurrent heart attacks or during preeclampsia and also in the seminal fluid of infertile men (30-32).

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Publications for IL-10 (DY522)(43)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: ELISA Capture.


Filter By Application
ELISA Capture
(1)
All Applications
Filter By Species
Human
(1)
Rat
(43)
All Species
Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using DY522 Applications Species
Anttila, JE;Mattila, OS;Liew, HK;Mätlik, K;Mervaala, E;Lindholm, P;Lindahl, M;Lindsberg, PJ;Tseng, KY;Airavaara, M; MANF protein expression is upregulated in immune cells in the ischemic human brain and systemic recombinant MANF delivery in rat ischemic stroke model demonstrates anti-inflammatory effects Acta neuropathologica communications 2024-01-16 [PMID: 38229173] (Rat) Rat
Xiao, Q;Chen, YH;Chen, YL;Chien, YS;Hsieh, LH;Shirakawa, H;Yang, SC; Potential Benefits of Epidermal Growth Factor for Inhibiting Muscle Degrative Markers in Rats with Alcoholic Liver Damage International journal of molecular sciences 2023-05-16 [PMID: 37240190] (Rat) Rat
L Li, ZC Yao, A Parian, YH Yang, J Chao, J Yin, KJ Salimian, SK Reddy, A Zaheer, SL Gearhart, HQ Mao, FM Selaru A nanofiber-hydrogel composite improves tissue repair in a rat model of Crohn's disease perianal fistulas Science Advances, 2023-01-04;9(1):eade1067. 2023-01-04 [PMID: 36598982] (Rat) Rat
SB Silva, K Honorato-S, SP Costa, TE Domingues, TMM da Cruz, CM Rodrigues, KB Costa, JM Dos Santos, VK da Silva L, TP Gaiad, AP Santos, MF Dias-Peixo, CC Coimbra, AM Dos Reis, RE Szawka, PHS Figueiredo, HS Costa, MX Oliveira, VA Mendonça, ACR Lacerda The superior beneficial effects of exercise training versus hormone replacement therapy on skeletal muscle of ovariectomized rats Scientific Reports, 2022-05-24;12(1):8764. 2022-05-24 [PMID: 35610295] (Rat) Rat
MJ Jeong, S Jeon, HS Yu, WS Cho, S Lee, D Kang, Y Kim, YJ Kim, SY Kim Exposure to Nickel Oxide Nanoparticles Induces Acute and Chronic Inflammatory Responses in Rat Lungs and Perturbs the Lung Microbiome International Journal of Environmental Research and Public Health, 2022-01-04;19(1):. 2022-01-04 [PMID: 35010784] (Rat) Rat
Q Xiao, YH Chen, SA Pratama, YL Chen, H Shirakawa, HC Peng, SC Yang The Prophylactic Effects of Glutamine on Muscle Protein Synthesis and Degradation in Rats with Ethanol-Induced Liver Damage Nutrients, 2021-08-14;13(8):. 2021-08-14 [PMID: 34444950] (Rat) Rat
X Xu, D Wang, Z Han, B Wang, W Gao, Y Fan, F Li, Z Zhou, C Gao, J Xiong, S Zhou, S Zhang, G Yang, R Jiang, J Zhang A novel rat model of chronic subdural hematoma: Induction of inflammation and angiogenesis in the subdural space mimicking human-like features of progressively expanding hematoma Brain research bulletin, 2021-04-28;172(0):108-119. 2021-04-28 [PMID: 33932488] (Rat) Rat
JNB Pereira, GM Murata, FT Sato, AR Marosti, CRO Carvalho, R Curi Small intestine remodeling in male Goto-Kakizaki rats Physiological Reports, 2021-02-01;9(3):e14755. 2021-02-01 [PMID: 33580916] (Rat) Rat
SM Davis, LA Collier, SJ Messmer, KR Pennypacke The Poststroke Peripheral Immune Response Is Differentially Regulated by Leukemia Inhibitory Factor in Aged Male and Female Rodents Oxidative Medicine and Cellular Longevity, 2020-12-10;2020(0):8880244. 2020-12-10 [PMID: 33376583] (Rat) Rat
YL Chen, H Shirakawa, NS Lu, HC Peng, Q Xiao, SC Yang Impacts of fish oil on the gut microbiota of rats with alcoholic liver damage J. Nutr. Biochem., 2020-09-11;86(0):108491. 2020-09-11 [PMID: 32920090] (Rat) Rat
Show All 43 Publications.

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Bioinformatics

Gene Symbol Il10