Rat CCL20/MIP-3 alpha DuoSet ELISA, 15 Plate

• Reactivity: Rt
• Applications: ELISA
• Conjugate: Biotin

Rat CCL20/MIP-3 alpha DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Ccl20

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat CCL20/MIP-3 alpha DuoSet ELISA, 15 Plate

• beta chemokine exodus-1
• Beta-chemokine exodus-1
• CC chemokine LARC
• C-C motif chemokine 20
• CCL20
• chemokine (C-C motif) ligand 20
• CKb4
• exodus-1
• LARC
• LARCLiver and activation-regulated chemokine
• MIP3 alpha
• MIP-3 alpha
• MIP-3a
• MIP-3-alpha
• MIP3AMacrophage inflammatory protein 3 alpha
• SCYA20Small-inducible cytokine A20
• small inducible cytokine subfamily A (Cys-Cys), member 20
• ST38

Background

CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.

Publications for CCL20/MIP-3 alpha (DY540)(6)

Publications using DY540

• TR Goesch, NA Wilson, W Zeng, BM Verhoven, W Zhong, MM Coumbe Git, WE Fahl Suppression of Inflammation-Associated Kidney Damage Post-Transplant Using the New PrC-210 Free Radical Scavenger in Rats Biomolecules, 2021-07-19;11(7):. 2021-07-19 [PMID: 34356678] (Rat)
• VGB Brito, MS Patrocinio, MCL Sousa, AEA Barreto, SCT Frasnelli, VS Lara, CF Santos, SHP Oliveira Mast cells contribute to alveolar bone loss in Spontaneously Hypertensive Rats with periodontal disease regulating cytokines production PLoS ONE, 2021-03-04;16(3):e0247372. 2021-03-04 [PMID: 33661916] (Rat)
• HJ Tsai, CC Shih, KY Chang, MH Liao, WJ Liaw, CC Wu, CM Tsao Angiotensin-(1-7) treatment blocks lipopolysaccharide-induced organ damage, platelet dysfunction, and IL-6 and nitric oxide production in rats Scientific Reports, 2021-01-12;11(1):610. 2021-01-12 [PMID: 33436885] (Rat)
• Burke S, May A, Noland R, Lu D, Brissova M, Powers A, Sherrill E, Karlstad M, Campagna S, Stephens J, Collier J Thiobenzothiazole-modified Hydrocortisones Display Anti-inflammatory Activity with Reduced Impact on Islet beta-Cell Function. J Biol Chem, 2015-04-07;290(21):13401-16. 2015-04-07 [PMID: 25851902] (Rat)
• Crane-Godreau MA, Wira CR Effects of estradiol on lipopolysaccharide and Pam3Cys stimulation of CCL20/macrophage inflammatory protein 3 alpha and tumor necrosis factor alpha production by uterine epithelial cells in culture. Infect. Immun., 2005-07-01;73(7):4231-7. 2005-07-01 [PMID: 15972514] (Rat)
• Crane-Godreau MA, Wira CR CCL20/macrophage inflammatory protein 3alpha and tumor necrosis factor alpha production by primary uterine epithelial cells in response to treatment with lipopolysaccharide or Pam3Cys. Infect. Immun., 2005-01-01;73(1):476-84. 2005-01-01 [PMID: 15618187] (Rat)

FAQs for CCL20/MIP-3 alpha (DY540). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Ccl20