RASAL1 Antibody

Western Blot: RASAL1 Antibody [NB100-55388] - (0.3ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RASAL1 Antibody Summary

• Immunogen: Peptide with sequence C-QERGKAALGPLGP corresponding to C-Terminus according to NP_004649.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RASAL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in human thyroid gland lysates (calculated MW of 90.0 kDa band according to NP_004649.1). An additional band of unknown identity was also consistently observed at 21 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  Human Thyroid Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RASAL1 Antibody

• RAS protein activator like 1 (GAP1 like)
• RASALGAP1 like protein
• rasGAP-activating-like protein 1

Background

The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RASAL1 Antibody (NB100-55388)(2)

Publications using NB100-55388

• �kmen G, Giannetto D, Fazio F, Arslan K. Investigation of Pomegranate ( Punica granatum L.) Flowers’ Antioxidant Properties and Antibacterial Activities against Different Staphylococcus Species Associated with Bovine Mastitis Veterinary Sciences 2023-06-11 [PMID: 37368780]
• Allen M, Chu S, Brill S et al. Restricted tissue expression pattern of a novel human rasGAP-related gene and its murine ortholog. Gene 1998-09-18 [PMID: 9751798]

Bioinformatics

• Gene Symbol: RASAL1
• Entrez:
  • 8437(Human)
• Uniprot:
  • O95294(Human)