![Western Blot: RAG1 Antibody [NBP3-13070] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RAG1 antibody (NBP3-13070) diluted at 1:500.](http://images.novusbio.com/fullsize2/RAG1-Antibody-Western-Blot-NBP3-13070-img0001.jpg "Western Blot: RAG1 Antibody [NBP3-13070] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RAG1 antibody (NBP3-13070) diluted at 1:500.")
| Reactivity | Hu, Mu, Rt, Po, Rb, RMSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human RAG1. The exact sequence is proprietary. |
| Predicted Species | Mouse (96%), Rat (95%), Porcine (90%), Rabbit (96%), Rhesus Macaque (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RAG1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol (pH7) |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Immunocytochemistry/Immunofluorescence: RAG2 Antibody [NBP2-43666] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAG2 protein stained by RAG2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10um.](http://images.novusbio.com/images2/RAG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-43666-img0005.jpg)
![Immunohistochemistry-Paraffin: RAG2 Antibody [NBP2-43666] - Analysis of DLD1 Xenograft, using RAG2 antibody at 1:100 dilution.](http://images.novusbio.com/images2/RAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-43666-img0002.jpg)
![Western Blot: RAG2 Antibody [NBP2-43666] - Analysis of 30 ug whole cell lysate. A: Molt-4 7.5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/images2/RAG2-Antibody-Western-Blot-NBP2-43666-img0003.jpg)
![Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-Frozen-NBP1-19371-img0013.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-Paraffin-NBP1-19371-img0014.jpg)
![Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/images2/CD4-Antibody-Flow-Cytometry-NBP1-19371-img0015.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunohistochemistry-Paraffin-NBP1-49045-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunocytochemistry-Immunofluorescence-NBP1-49045-img0006.jpg)
![Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.](http://images.novusbio.com/images2/CD8-Antibody-(53-6.7)-Immunohistochemistry-Frozen-NBP1-49045-img0008.jpg)
![Western Blot: TdT Antibody [NBP2-31368] - WB detection of TDT protein in (A) lysate of MOLT4 human leukemia cell line and (B) partial recombinant protein by using TDT antibody. Primary antibody concentration used: 1 ug/ml for Molt4 lysate, 0.05 ug/ml for recombinant protein.](http://images.novusbio.com/images2/TdT-Antibody-Western-Blot-NBP2-31368-img0001.jpg)
![Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368] - IHC-P analysis of TdT protein in a section of human lung using TdT antibody at a concentration of 10 ug/ml. The alveolar cells of the lungs showed strong nuclear / weak cytoplasmic positivity for TdT immunostaining.](http://images.novusbio.com/images2/TdT-Antibody-Immunohistochemistry-Paraffin-NBP2-31368-img0002.jpg)
![Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368] - IHC-P analysis of TdT protein in a section of human hepatocellular carcinoma (HCC) using TdT antibody at a concentration of 10 ug/ml. The HCC cells depicted strong nuclear / weak cytoplasmic positivity for TdT immunostaining.](http://images.novusbio.com/images2/TdT-Antibody-Immunohistochemistry-Paraffin-NBP2-31368-img0003.jpg)
![Immunohistochemistry: GRB10 Antibody [NB100-88153] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GRB10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/images2/GRB10-Antibody-Immunohistochemistry-NB100-88153-img0004.jpg)
![Immunohistochemistry-Paraffin: GRB10 Antibody [NB100-88153] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-GRB10 used at a dilution of 1:250](http://images.novusbio.com/images2/GRB10-Antibody-Immunohistochemistry-Paraffin-NB100-88153-img0001.jpg)
![Immunoprecipitation: GRB10 Antibody [NB100-88153] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-88153 used for IP at 3 mcg/mg lysate. GRB10 was also immunoprecipitated by rabbit anti- GRB10 antibody NB100-88152, which recognizes an upstream epitope.](http://images.novusbio.com/images2/GRB10-Antibody-Immunoprecipitation-NB100-88153-img0002.jpg)
![Immunohistochemistry-Paraffin: SEPN1 Antibody [NBP2-34098] - Staining of human lung shows strong cytoplasmic positivity in pneumocytes and macrophages.](http://images.novusbio.com/images2/SEPN1-Antibody-Immunohistochemistry-Paraffin-NBP2-34098-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SEPN1 Antibody [NBP2-34098] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/images2/SEPN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34098-img0006.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0014.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0015.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Protein Array: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA DQ/DR/DP Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Protein-Array-NBP2-79843-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79843-img0002.jpg)
![Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Western-Blot-NBP2-79843-img0003.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (30-F11) [NB100-77417] - Staining of a FFPE mouse liver tissue section using 1:100 dilution of CD45 antibody (30-F11) [NB100-77417]. Signal was detected using AF488 conjugated donkey anti-rat secondary antibody at 1:500 dilution. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/images2/CD45-Antibody-(30-F11)-Immunohistochemistry-Paraffin-NB100-77417-img0002.jpg)
![Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY licence.](http://images.novusbio.com/images2/CD45-Antibody-(30-F11)-Immunohistochemistry-NB100-77417-img0009.jpg)
![Immunohistochemistry: CD45 Antibody (30-F11) [NB100-77417] - Severe apoptosis and pathology in the orally infected heart. Experimental design is as outlined in Fig. 1B. Heart sections from moribund mice were examined with H&E, and immunohistochemistry staining for pan-leukocyte infiltration using an anti-CD45 antibody. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-019-47455-3), licensed under a CC-BY licence.](http://images.novusbio.com/images2/CD45-Antibody-(30-F11)-Immunohistochemistry-NB100-77417-img0012.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for RAG1 Antibody (NBP3-13070)Discover more about diseases related to RAG1 Antibody (NBP3-13070).
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| Gene Symbol | RAG1 |
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")